AUTHOR=Frydman Galit H. , Ellett Felix , Jorgensen Julianne , Marand Anika L. , Zukerberg Lawrence , Selig Martin K. , Tessier Shannon N. , Wong Keith H. K. , Olaleye David , Vanderburg Charles R. , Fox James G. , Tompkins Ronald G. , Irimia Daniel TITLE=Megakaryocytes respond during sepsis and display innate immune cell behaviors JOURNAL=Frontiers in Immunology VOLUME=14 YEAR=2023 URL=https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2023.1083339 DOI=10.3389/fimmu.2023.1083339 ISSN=1664-3224 ABSTRACT=
Megakaryocytes (MKs) are precursors to platelets, the second most abundant cells in the peripheral circulation. However, while platelets are known to participate in immune responses and play significant functions during infections, the role of MKs within the immune system remains largely unexplored. Histological studies of sepsis patients identified increased nucleated CD61+ cells (MKs) in the lungs, and CD61+ staining (likely platelets within microthrombi) in the kidneys, which correlated with the development of organ dysfunction. Detailed imaging cytometry of peripheral blood from patients with sepsis found significantly higher MK counts, which we predict would likely be misclassified by automated hematology analyzers as leukocytes. Utilizing