AUTHOR=Scuiller Yvonne , Hemon Patrice , Le Rochais Marion , Pers Jacques-Olivier , Jamin Christophe , Foulquier Nathan 

TITLE=YOUPI: Your powerful and intelligent tool for segmenting cells from imaging mass cytometry data

JOURNAL=Frontiers in Immunology

VOLUME=14

YEAR=2023

URL=https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2023.1072118

DOI=10.3389/fimmu.2023.1072118

ISSN=1664-3224

ABSTRACT=<p>The recent emergence of imaging mass cytometry technology has led to the generation of an increasing amount of high-dimensional data and, with it, the need for suitable performant bioinformatics tools dedicated to specific multiparametric studies. The first and most important step in treating the acquired images is the ability to perform highly efficient cell segmentation for subsequent analyses. In this context, we developed YOUPI (Your Powerful and Intelligent tool) software. It combines advanced segmentation techniques based on deep learning algorithms with a friendly graphical user interface for non-bioinformatics users. In this article, we present the segmentation algorithm developed for YOUPI. We have set a benchmark with mathematics-based segmentation approaches to estimate its robustness in segmenting different tissue biopsies.</p>