AUTHOR=Iwabuchi Sadahiro , Tsukahara Tomohide , Okayama Toshitugu , Kitabatake Masahiro , Motobayashi Hideki , Shichino Shigeyuki , Imafuku Tadashi , Yamaji Kenzaburo , Miyamoto Kyohei , Tamura Shinobu , Ueha Satoshi , Ito Toshihiro , Murata Shin-ichi , Kondo Toshikazu , Ikeo Kazuho , Suzuki Yutaka , Matsushima Kouji , Kohara Michinori , Torigoe Toshihiko , Yamaue Hiroki , Hashimoto Shinichi TITLE=B cell receptor repertoire analysis from autopsy samples of COVID-19 patients JOURNAL=Frontiers in Immunology VOLUME=14 YEAR=2023 URL=https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2023.1034978 DOI=10.3389/fimmu.2023.1034978 ISSN=1664-3224 ABSTRACT=

Neutralizing antibodies against the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) are being developed world over. We investigated the possibility of producing artificial antibodies from the formalin fixation and paraffin-embedding (FFPE) lung lobes of a patient who died by coronavirus disease 2019 (COVID-19). The B-cell receptors repertoire in the lung tissue where SARS-CoV-2 was detected were considered to have highly sensitive virus-neutralizing activity, and artificial antibodies were produced by combining the most frequently detected heavy and light chains. Some neutralizing effects against the SARS-CoV-2 were observed, and mixing two different artificial antibodies had a higher tendency to suppress the virus. The neutralizing effects were similar to the immunoglobulin G obtained from healthy donors who had received a COVID-19 mRNA vaccine. Therefore, the use of FFPE lung tissue, which preserves the condition of direct virus sensitization, to generate artificial antibodies may be useful against future unknown infectious diseases.