AUTHOR=Baliña-Sánchez Carmen , Aguilera Yolanda , Adán Norma , Sierra-Párraga Jesús María , Olmedo-Moreno Laura , Panadero-Morón Concepción , Cabello-Laureano Rosa , Márquez-Vega Catalina , Martín-Montalvo Alejandro , Capilla-González Vivian TITLE=Generation of mesenchymal stromal cells from urine-derived iPSCs of pediatric brain tumor patients JOURNAL=Frontiers in Immunology VOLUME=14 YEAR=2023 URL=https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2023.1022676 DOI=10.3389/fimmu.2023.1022676 ISSN=1664-3224 ABSTRACT=
Human induced pluripotent stem cells (iPSCs) provide a virtually inexhaustible source of starting material for next generation cell therapies, offering new opportunities for regenerative medicine. Among different cell sources for the generation of iPSCs, urine cells are clinically relevant since these cells can be repeatedly obtained by non-invasive methods from patients of any age and health condition. These attributes encourage patients to participate in preclinical and clinical research. In particular, the use of urine-derived iPSC products is a convenient strategy for children with brain tumors, which are medically fragile patients. Here, we investigate the feasibility of using urine samples as a source of somatic cells to generate iPSC lines from pediatric patients with brain tumors (BT-iPSC). Urinary epithelial cells were isolated and reprogrammed using non-integrative Sendai virus vectors harboring the Yamanaka factors