AUTHOR=Siekman Sterre L. , Pongracz Tamas , Wang Wenjun , Nouta Jan , Kremsner Peter G. , da Silva-Neto Pedro Vieira , Esen Meral , Kreidenweiss Andrea , Held Jana , Trapé Átila Alexandre , Fendel Rolf , de Miranda Santos Isabel Kinney Ferreira , Wuhrer Manfred ,  ImmunoCovid Consortium , Amorim Alessandro P. de , Argolo Jamille G M. , Barbieri Rita de C.C. , Dias-Baruffi Marcelo , Bastos Victor A F. , Bonat Vânia L D. , Cardoso Cristina Ribeiro de Barros , Carmona-Garcia Ingryd , Carvalho Jonatan C S de , Constant Leticia F. , Degiovani Augusto M. , Dias Cassia F.S.L. , Faccioli Lúcia H. , Feitosa Marley R. , Feres Omar , Fernandes Ana Paula Morais , Fernandes Talita M. , Fraga-Silva Thais F C. , Fuzo Carlos , Guarneri Isabelle C. , Milanezi Cristiane M. , Garbato Caroline T. , Gaspar Gilberto Gambero , Júnior Ângelo A.F. , Maruyama Sandra R. , Nepomuceno Debora C. , Neto Nicola T. , Oliveira Camilla Narjara Simão , Ostini Fátima M. , Parra Rogerio S. , Pérez Malena M. , Pimentel Vinı́cius E. , Porcel Giovanna da S. , Rocha José J R da , Rodrigues Lilian C. , Russo Elisa M S. , Silva Dayane P. da , Silva Rafael C. da , Sorgi Carlos Arterio , Souza Camila O S. , Toro Diana M. , Viana Angelina L. , Vilar Fernando Crivelenti , Xavier Ana C. , Zaparoli Kamila 

TITLE=The IgG glycome of SARS-CoV-2 infected individuals reflects disease course and severity

JOURNAL=Frontiers in Immunology

VOLUME=13

YEAR=2022

URL=https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2022.993354

DOI=10.3389/fimmu.2022.993354

ISSN=1664-3224

ABSTRACT=<p>Immunoglobulin G (IgG) antibodies play an important role in the immune response against viruses such as SARS-CoV-2. As the effector functions of IgG are modulated by <italic>N</italic>-glycosylation of the Fc region, the structure and possible function of the IgG <italic>N</italic>-glycome has been under investigation in relation to divergent COVID-19 disease courses. Through LC-MS analysis we studied both total IgG1 and spike protein-specific IgG1 Fc glycosylation of 129 German and 163 Brazilian COVID-19 patients representing diverse patient populations. We found that hospitalized COVID-19 patients displayed decreased levels of total IgG1 bisection and galactosylation and lowered anti-S IgG1 fucosylation and bisection as compared to mild outpatients. Anti-S IgG1 glycosylation was dynamic over the disease course and both anti-S and total IgG1 glycosylation were correlated to inflammatory markers. Further research is needed to dissect the possible role of altered IgG glycosylation profiles in (dys)regulating the immune response in COVID-19.</p>