AUTHOR=Loganathan Aravinthan , McMorrow Fionnuala , Lu Hui , Li Danyang , Mulhearn Ben , McHugh Neil John , Tansley Sarah Louise 

TITLE=The use of ELISA is comparable to immunoprecipitation in the detection of selected myositis-specific autoantibodies in a European population

JOURNAL=Frontiers in Immunology

VOLUME=13

YEAR=2022

URL=https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2022.975939

DOI=10.3389/fimmu.2022.975939

ISSN=1664-3224

ABSTRACT=<sec><title>Background</title><p>The reliable detection of myositis-specific autoantibodies (MSA) provides valuable clinical information regarding prognosis, clinical progression and diagnostic confirmation.</p></sec><sec><title>Objectives</title><p>To evaluate the reliability of a commercial ELISA immunoassay in detecting myositis-specific autoantibodies in comparison to immunoprecipitation as the reference standard.</p></sec><sec><title>Methods</title><p>Serum samples were chosen from a biobank of more than 3000 samples. Samples with a confirmed MSA on Immunoprecipitation (n=116) were evaluated in duplicate by ELISA to detect Mi2, MDA5, Jo1, EJ, KS, PL-7 and PL-12 (Medical &amp; Biological Laboratories Co. Ltd, Nagoya, Aichi, Japan). Healthy control samples (n=246) confirmed autoantibody negative by immunoprecipitation were similarly assessed.</p></sec><sec><title>Results</title><p>There was a very good agreement between ELISA and immunoprecipitation for serum samples containing anti-Mi2, MDA5, Jo1, EJ, KS and PL-7 and PL-12 auto-antibodies. Cohen’s κ values ranged from 0.86-1 for the measured autoantibodies on ELISA.</p></sec><sec><title>Conclusion</title><p>ELISA was an accurate method for detecting anti-synthetase, anti-Mi2 and anti-MDA5 autoantibodies.</p></sec>