AUTHOR=Wang Tiecheng , Miao Faming , Lv Shengnan , Li Liang , Wei Feng , Hou Lihua , Sun Renren , Li Wei , Zhang Jian , Zhang Cheng , Yang Guang , Xiang Haiyang , Meng Keyin , Wan Zhonghai , Wang Busen , Feng Guodong , Zhao Zhongpeng , Luo Deyan , Li Nan , Tu Changchun , Wang Hui , Xue Xiaochang , Liu Yan , Gao Yuwei TITLE=Proteomic and Metabolomic Characterization of SARS-CoV-2-Infected Cynomolgus Macaque at Early Stage JOURNAL=Frontiers in Immunology VOLUME=13 YEAR=2022 URL=https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2022.954121 DOI=10.3389/fimmu.2022.954121 ISSN=1664-3224 ABSTRACT=

Although tremendous effort has been exerted to elucidate the pathogenesis of severe COVID-19 cases, the detailed mechanism of moderate cases, which accounts for 90% of all patients, remains unclear yet, partly limited by lacking the biopsy tissues. Here, we established the COVID-19 infection model in cynomolgus macaques (CMs), monitored the clinical and pathological features, and analyzed underlying pathogenic mechanisms at early infection stage by performing proteomic and metabolomic profiling of lung tissues and sera samples from COVID-19 CMs models. Our data demonstrated that innate immune response, neutrophile and platelet activation were mainly dysregulated in COVID-19 CMs. The symptom of neutrophilia, lymphopenia and massive “cytokines storm”, main features of severe COVID-19 patients, were greatly weakened in most of the challenged CMs, which are more semblable as moderate patients. Thus, COVID-19 model in CMs is rational to understand the pathogenesis of moderate COVID-19 and may be a candidate model to assess the safety and efficacy of therapeutics and vaccines against SARS-CoV-2 infection.