Peter Brossart- Department of Internal Medicine Oncology, Hematology, Immuno-Oncology and Rheumatology/Clinical Immunology, University of Bonn, Bonn, Germany
Editorial on the Research Topic
Versatile Roles for B Cells in Tumor Immunity
The present Research Topic titled ”Versatile Roles for B Cells in Tumor Immunity” features 6 articles that highlight the crucial role of B cell elicited effects on the generation of an effective immunity to combat cancer.
The tremendous clinical success of immune checkpoint blockade in the treatment of malignant diseases has confirmed the importance of T cells. However, the role of B cells is increasingly recognized and there is abundant evidence that tumor-infiltrating B lymphocytes (TIL-Bs) have an important and synergistic impact in the control and elimination of malignant cells. They can mediate a powerful anti-tumor immunity that results from antibody production, a unique mode of antigen presentation, release of cytokines and chemokines and their central role in the assembling of the tumor microenvironments (TME). The presence of TIL-Bs has been shown to be a strong prognostic and predictive factor for the efficacy of immune checkpoint blockade or standard therapies (1, 2).
Singh et al investigated in the B16 melanoma mouse model the role of B cells in generating a protective immunity in response to PD-L1 blockade alone or in combination with TLR-7/8 activation. In this model, B cells and the B cell derived chemokine CXCL13 were required to promote the efficacy of anti-PD-L1 alone or in combination with a TLR-7/8 agonist. In contrast, B cells were dispensable for the therapeutic effect of anti-CTLA-4 inhibition. CD40-positive but not CD40-negative B cells contributed to anti-melanoma immunity. Further analyses of melanoma patients’ TCGA data confirmed that the presence of B cells and the CXCL13 chemokine together with CD8+ T cells were strongly associated with improved overall survival.
Conventional B cells arise and mature from the common lymphocyte progenitor lineage in bone marrow, where they undergo V(D)J recombination, resulting in cell surface expression of B cell receptors of the IgM isotype. They migrate from the bone marrow to the spleen and differentiate to marginal B cells that remain in the spleen or follicular B cells that populate the lymph nodes. Upon contact with the cognate antigen, B cells further undergo affinity maturation and class switch recombination that improves antigen specificity. Most of the immature B cells are self-reactive and undergo clonal deletion or receptor editing. However, about 20% of naive mature B lymphocytes exert some degree of self-reactivity and enter the periphery. Peripheral B cell tolerance is mainly maintained by the suppressive effects of CD4+ regulatory T cells. Thus, the antigenic repertoires and mechanisms mediating central and peripheral tolerance in B cells differ from T cells and there are several reports demonstrating that TIL-Bs can recognize self-antigens. Human TIL-Bs express HLA class I and II and co-stimulatory molecules and have fundamental implications for processing of tumor derived antigens and the generation of humoral and cellular anti-cancer immunity. In addition, B cells play a central and integral role in epitope spreading (3). Similar to normal B cells TIL-Bs produce various immunostimulatory chemokines and cytokines, including IL-4, IL-6, IFNγ, TNF, C–C motif chemokine ligand 3 (CCL3), IL-2 and GM-CSF. Recent data indicate that tumor tissues contain highly diverse populations of B cells at different developmental states (1, 2).
Yu et al. addressed this topic by extracting the B cell repertoires and populations from 28 different cancer types in the TCGA dataset. They found similar V-gene usage patterns in colorectal and endometrial cancers and significant associations with survival in a subset of tumor types. Although large differences were present in the tumor entities with their B cell repertoire, there were significant associations between the B cell repertoire features and mutation load, tumor stage, and age in a few tumor types.
In the TME TIL-Bs are mostly found in close neighborhood to different T cell populations, NK cells and myeloid cells that can result in the formation of ectopic aggregates of immune cells. These tertiary lymphoid structures (TLSs) display a lymph node-like composition with B- and T cell zones and play a central role in initiating and maintaining of adaptive immune responses (2, 4, 5). Germain et al. analyzed the relationship between TSL-B cells and CD4+ T cells in a prospective cohort of 56 NSCLC patients. They observed that tumor-infiltrating T cells showed marked differences according to TLS-B cell presence. A retrospective analysis of 538 untreated NSCLC patients revealed that high TLS-B cell presence counterbalanced the deleterious effect of high Treg density on patient survival, and that TLS-Bhigh Treglow patients had the best clinical outcomes indicating that B cells may play a central role in promoting protective T cell responses. In addition, Wu et al. found that CD20+CD22+ADAM28+ B cells in cancer-associated TLS are involved in the efficacy of check point inhibition and promote the response to therapy. B-cell density was predictive for a response to checkpoint inhibition.
Regulatory B cells (Breg) have been described in malignant tumors characterized by the secretion of IL-10. However, according to recent studies regulatory B cells also produce IL-35, granzyme B and TGF-β. Breg can develop from memory B cells, plasmablasts and plasmocytes. In contrast to regulatory T cells Breg cells lack a lineage marker such as forkhead box protein P3 and are defined by their effector molecules. Breg induce Treg polarization, inhibit the effector functions of CD4+ and CD8+ T cells and antigen-presenting cells. The presence of Breg in tumor tissues has been associated with a poor prognosis (1).
Michaud et al. used a novel IL-35 reporter model to determine the signaling pathways that promote the immunosuppressive effects of regulatory B cells. In vitro analysis of IL-35 reporter B cells revealed that the combined BCR and TLR4 signaling is capable of inducing IL-35 expression. However, in vivo, B cell receptor activation was central to B cell-mediated suppression and promotion of pancreatic cancer growth. Further analysis revealed protein kinase D2 (PKD2) as a key downstream regulator of IL-35 expression in B cells. Blocking of PKD in B cells inhibited growth of cancer cells and enhanced the effector T cell function upon adoptive transfer into B cell-deficient mice. These data might help to identify novel therapeutic targets to attenuate the function of regulatory B cells.
Author Contributions
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Conflict of Interest
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References
1. Laumont CM, Banville AC, Gilardi M, Hollern DP, Nelson BH. Tumour-Infiltrating B Cells: Immunological Mechanisms, Clinical Impact and Therapeutic Opportunities. Nat Rev Cancer (2022). doi: 10.1038/s41568-022-00466-1
2. Lauss M, Donia M, Svane IM, Jönsson G. B Cells and Tertiary Lymphoid Structures: Friends or Foes in Cancer Immunotherapy? Clin Cancer Res (2022) 28(9):1751–8. doi: 10.1158/1078-0432.CCR-21-1130
3. Brossart P. The Role of Antigen Spreading in the Efficacy of Immunotherapies. Clin Cancer Res (2020) 26(17):4442–7. doi: 10.1158/1078-0432.CCR-20-0305
4. Garaud S, Dieu-Nosjean MC, Willard-Gallo K. T Follicular Helper and B Cell Crosstalk in Tertiary Lymphoid Structures and Cancer Immunotherapy. Nat Commun (2022) 13(1):2259. doi: 10.1038/s41467-022-29753-z
Keywords: tertiary lymphoid structures, tumor microenvironment, antigen presentation, tumor immunology, cancer, tumor infiltrating B cells, regulatory B lymphocytes, immunotherapy
Citation: Brossart P (2022) Editorial: Versatile Roles for B Cells in Tumor Immunity. Front. Immunol. 13:951933. doi: 10.3389/fimmu.2022.951933
Received: 24 May 2022; Accepted: 30 May 2022;
Published: 17 June 2022.
Edited and reviewed by:
Katy Rezvani, University of Texas MD Anderson Cancer Center, United StatesCopyright © 2022 Brossart. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Peter Brossart, peter.brossart@ukb.uni-bonn.de