Kensuke Miyake
Takuma Shibata
Ryutaro Fukui
Ryota Sato1
Yusuke Murakami- 1Division of Innate Immunity, Department of Microbiology and Immunology, The Institute of Medical Science, The University of Tokyo, Minato-ku, Japan
- 2Faculty of Pharmacy, Department of Pharmaceutical Sciences and Research Institute of Pharmaceutical Sciences, Musashino University, Tokyo, Japan
Toll-like receptors (TLRs) respond to pathogen constituents, such as microbial lipids and nucleic acids (NAs). TLRs recognize NAs in endosomal compartments. Structural and functional studies have shown that recognition of NAs by TLRs depends on NA processing by RNases and DNases. DNase II-dependent DNA degradation is required for TLR9 responses to single-stranded DNAs, whereas RNase T2-dependent RNA degradation enables TLR7 and TLR8 to respond to nucleosides and oligoribonucleotides. In contrast, RNases and DNases negatively regulate TLR responses by degrading their ligands. RNase T2 negatively regulates TLR3 responses to degrading the TLR3 ligand double-stranded RNAs. Therefore, NA metabolism in the endosomal compartments affects the endosomal TLR responses. Dysregulation of NA metabolism in the endosomal compartment drives the TLR-dependent pathologies in human diseases.
1 Introduction
The Toll family of receptors are expressed in innate immune cells, such as macrophages and dendritic cells (DCs), and respond to pathogen components to activate defense responses during bacterial and viral infections. Nucleic acids (NAs) are sensed by a subfamily of toll-like receptors (TLRs) including TLR3, TLR7, TLR8, TLR9, and TLR13. These NA-sensing TLRs are localized in the endosomal compartment to prevent hazardous autoimmune responses (1). NA degradation in the endosomal compartment negatively regulates TLR responses to self-derived NAs. However, evidence for another reason of TLR localization to the endosomal compartment is emerging. Structural and functional studies have shown that NA-sensing TLRs sense NA-degradation products, such as oligonucleotides and nucleosides (2–4), demonstrating that NA degradation generates TLR ligands. NA metabolism in the endosomal compartment is considered a positive and negative regulator of NA-sensing TLRs.
The endosomal compartment affects downstream signaling as well as NA sensing. TLRs activate two signaling pathways: proinflammatory signals activating NF-κB transcription factors and type I interferon signals activating transcription factors called interferon regulatory factors (IRFs) (5, 6). Both signals are activated in a mutually exclusive manner with the former preceding the latter pathway. Delayed activation of IRFs is ascribed to the requirement of endosomal trafficking for IRF activation by TLRs. Therefore, endosomal trafficking serves as a switch to change TLR responses from proinflammatory to type I interferon (IFN) responses.
Constitutive activation of NA-sensing TLRs causes inflammatory diseases. Constitutive TLR activation is caused by alteration in NA metabolism, the endosomal compartment, or downstream signaling. These inflammatory diseases reveal molecular and cellular mechanisms by which endosomal TLRs are controlled by the endosomal compartment (1, 7).
Here, we provide an overview of recent progress in our understanding of the mechanisms by which endosomal TLR responses are controlled and the diseases caused by dysregulation of these controlling mechanisms.
1.1 Nucleic Acid Recognition by TLRs in the Endosomal Compartment
1.1.1 TLR3
TLR3 responds to double-stranded RNAs (dsRNAs) longer than 40–50 bp (8); this length is required to interact with a pair of TLR3 molecules and induce their dimerization. However, it remains unclear whether longer dsRNAs induce stronger TLR3 responses. TLR3 is expressed not only in the innate immune cells, such as macrophages and dendritic cells, but also in non-immune cells, such as neurons and keratinocytes. Broad expression enables TLR3 to serve as a sentinel protein in non-immune cells. For example, loss-of-function mutations in the genes required for TLR3-dependent type I IFN responses increase susceptibility to herpes encephalitis (9). Because neurons express only a limited set of pathogen sensors, TLR3 expressed in neurons plays an indispensable role in the control of herpes virus infection. TLR3 is probably activated by dsRNAs of virus origin, of which expression increase during viral infection. TLR3 also responds to self-derived RNAs during tissue damage. In keratinocytes, TLR3 responds to self-derived U1 RNA released from UV-irradiated cells to promote tissue repair (10). In contrast, TLR3 plays a pathologic role in radiation-induced gastrointestinal syndrome (11). TLR3 expressed in intestinal crypt cells responds to dsRNAs released p53-dependently from irradiated cells. Expression of TLR3 in crypt cells causes cell death and exacerbates radiation-induced gastrointestinal syndromes. Broad expression and responses to self-derived dsRNAs allow TLR3 to serve as a sensor not only for viruses but also for various tissue damage.
1.1.2 TLR7, TLR8, and TLR13
TLR7 and TLR8 are known to respond to single-stranded RNAs (ssRNAs), but their structural and functional analyses have shown that these TLRs bind to nucleosides and oligoribonucleotides (2–4). TLR7 is activated by guanosine or deoxyguanosine along with oligoribonucleotides, whereas TLR8 responds to uridine and oligoribonucleotides. Nucleosides and oligonucleotides synergistically activate both TLRs because oligoribonucleotides enhance TLR7/8 affinity to nucleosides. TLR7 and TLR8, therefore, respond to RNA degradation products generated in the endosomal compartment. This is a strong reason for the localization of TLR7 and TLR8 in the endosomal compartment. In mice, TLR8 is not active, but TLR13 serves as a bona fide ssRNA sensor. TLR13 binds directly to bacterial 23S ribosomal RNA in a sequence-specific manner (12, 13).
1.1.3 TLR9
TLR9 responds to single-stranded DNAs (ssDNAs). Because TLR9 has two binding sites, ssDNA fragment binds to a pair of TLR9 molecules, leading to the formation of a TLR9 dimer with two ssDNA fragments (14). Because cell surface expression of TLR9 drives systemic inflammation (15), ssDNA fragments may be present in the extracellular space as well as in the endosomal compartment.
1.2 Effect of Nucleic Acid Metabolism on Endosomal TLR Responses
1.2.1 DNase I and DNase I-Like 3
NAs released from dead cells are internalized into the endosomal compartment of the macrophages. Extracellular DNA is degraded by members of the DNASE1 family, such as DNase I and DNase I-like 3 (Figure 1). Because these enzymes require an optimal pH of 7.0, they degrade DNA before internalization into the endosomal compartment. DNase I is expressed in the kidney and lacrimal gland, whereas DNase I-like 3 is expressed in the innate immune cells, such as DCs. Despite their restricted expression, DNases are secreted and can degrade DNA in the circulation (16). Lupus-like diseases develop in patients harboring loss-of-function mutations in DNASE1 or DNASE1L3 genes (17, 18). Consistent with this, Dnse1l3 deficiency in mice causes TLR7 and TLR9-dependent systemic autoimmune response (19). TLR7 activation in Dnse1l3−/− mice may be explained by TLR7 response to DNA-derived deoxyribonucleosides (2). Although DNase I is thought to negatively regulates TLR responses to self-DNA, TLR-dependency of lupus nephritis in Dnase1−/− mice has not been shown yet.
Figure 1 Processing or degradation of TLR ligands by DNases and RNases. The extracellular and lysosomal enzymes involved in NA metabolism are shown. The Table summarizes the role of each enzyme in TLR responses. RNase T2 negatively regulates TLR3 responses but is required for TLR7 and TLR8 responses. PLD3, PLD4, and DNase I-like 3 negatively regulate TLR7, TLR8, and TLR9 responses. DNase 2 is required for TLR9 response.
1.2.2 DNase II
DNase II is expressed in various cell types and exhibits optimal activity at pH 5.0. It plays an indispensable role in DNA degradation in the endosomal compartment. Loss-of-function mutations in the DNASE2 gene cause type I interferonopathy characterized by anemia, thrombocytopenia, hepatosplenomegaly, glomerulonephritis, and liver fibrosis (20). Consistent with this, Dnase2a−/− mice are embryonically lethal owing to type I IFN-dependent anemia (21). Type I IFN production is driven by the cGAS-STING axis, because Dnase2a−/− Sting−/− mice are born normal (22). Although DNA accumulates in the endosomal compartment, TLR9 is not involved in type II IFN-dependent lethality (23) because DNase II is required for generation of a TLR9 ligand in DCs (24). Dnase2a−/− mice rescued by type I IFN receptor deficiency suffer from arthritis due to the constitutive activation of cGAS-STING and another cytoplasmic dsDNA sensor absent in melanoma 2 (AIM2) (25). The activation of cytoplasmic dsDNA sensors in Dnase2a−/− mice raises the question of how lysosomal DNA enters the cytoplasm. dsDNAs are released from the nucleus to the cytoplasm under various stresses, and cytoplasmic dsDNAs are degraded by endosomal DNase II (26). cGAS-STING and AIM2 may be activated by nuclear DNA, which escapes lysosomal degradation in Dnase2a−/− mice.
1.2.3 RNase T2
RNase T2, the member of the T2 family of RNases with optimal activity at pH 4–5, is broadly expressed in various cell types. RNase T2 degrades RNA in the endosomal compartment, such as ribosomal RNA (27, 28). Loss-of-function mutations in the RNASET2 gene cause cystic leukoencephalopathy (29), and RNase T2-deficient mice show type I interferonopathy; however, the responsible RNA sensor remains unclear (30). RNase T2 negatively regulates TLR3 responses by degrading dsRNAs, whereas it is required for TLR7/8 responses via the generation of ligands (31–33). These RNA-sensing TLRs might play a role in cystic leukoencephalopathy.
1.2.4 Phospholipase D3 and Phospholipase D4
Phospholipase D3 (PLD3) and Phospholipase D4 (PLD4) belong to the phospholipase D family. Macrophages express both PLD3 and PLD4, whereas B cells and DCs express only PLD4. Genome-wide association studies have shown that the PLD4 gene is linked to autoimmune diseases, such as systemic sclerosis, systemic lupus erythematosus (SLE), and rheumatoid arthritis (34–36). In contrast, the PLD3 gene is linked to neurodegenerative diseases, such as Alzheimer’s disease and spinocerebellar ataxia (37, 38). Pld3−/− Pld4−/− mice exhibit macrophage activation syndrome (39, 40). PLD3 and PLD4 exonucleases degrade both DNA and RNA and negatively regulate TLR7 and TLR9 responses. The constitutive activation of TLR7 and TLR9 contributes to the pathology in Pld3−/− Pld4−/− mice (39).
1.3 Endosomal Compartment as the Platform Controlling Endosomal TLRs
1.3.1 Unc93B1
Unc93B1 is a multi-transmembrane endoplasmic reticulum (ER) molecule that is directly associated with the endosomal TLRs, including TLR3, TLR5, TLR7, TLR8, TLR9, and TLR13 (Figure 2). Without Unc93B1, these TLRs remain in the ER and fail to respond to their cognate ligands (41). In addition to its role as a TLR-specific chaperone, Unc93B1 directly affects TLR response. For example, Unc93B1 dissociates from TLR9 and TLR3 upon ligand stimulation. If Unc93B1 stays with TLR9 and TLR3, these TLRs fail to dimerize and activate downstream signals (42, 43). In TLR7, Unc93B1 remains associated with ligated TLR7, but the complex is degraded after being transported into intralumenal vesicles (44). These results demonstrated the role of Unc93B1 as a negative regulator of endosomal TLR response by inhibiting dimerization or degradation. The D34A mutation of Un93B1 in mice causes systemic inflammation due to constitutive TLR7 activation (45), suggesting that Unc93B1 serves as a negative regulator of TLR7 at the steady state. However, little is known about the mechanism by which Unc93B1 dissociates from TLR9 or TLR3 upon ligand stimulation.
Figure 2 Endosomal molecules controlling TLR responses. Endosomal molecules that control TLR responses are shown. Unc93B1 negatively regulates TLR9 dimerization. A complex consisting of SLC15A4 and TASL mediates TLR-dependent type I IFN production.
1.3.2 Mechanisms of Type I IFN Production
TLR-dependent type I IFN production is controlled by the endosomal compartment in multiple ways. For instance, the endosomal compartment is the site where metabolic information is gathered. It is not surprising that metabolic sensors, such as mammalian target of rapamycin complex 1 (mTORC1), are localized in the endosomal compartment. Type I IFN production by TLR7 or TLR9 in pDCs is dependent on mTORC1 activation. Interestingly, proinflammatory cytokine production does not depend on mTORC1. Because mTORC1 activation drives anabolic processes in immune cells, type I IFN response might be more dependent on the anabolic activity than on proinflammatory responses.
TLR-dependent type I IFN induction is preceded by the stimulation of proinflammatory cytokines (46). This delayed type I IFN induction is ascribed to the requirement of endosomal trafficking for type I IFN production (47). Endosomal trafficking depends on small GTPases such as ADP ribosylation factors like 8b (Arl8b) and Rab7a. Interestingly, these proteins are differentially activated by TLRs. For example, TLR7 trafficking in pDCs depends on Arl8b, whereas TLR3 trafficking is regulated by Rab7a (46, 48). These GTPases mediate anterograde trafficking of TLR-containing endosomes from perinuclear regions to the cell periphery. Endosomal trafficking enables TLRs to interact with mTORC1 (49), suggesting that such trafficking connects the metabolic status with type I IFN responses.
TLR7 activation in pDCs initiates inside-out signaling of αLβ2 integrin, the adhesion of which is required to induce endosomal trafficking (46). Consistent with this, cell-cell interactions enhance type I IFN production by pDCs (50). The initiation of type I IFN responses is likely to depend on the optimal environment, such as the anabolic process and cell adhesion. TLRs sense these environmental conditions through endosomal trafficking. In other words, environmental cues affect TLR-dependent type I IFN responses via endosomal positioning.
SLC15A3 and SLC15A4 are peptide transporters in endosomal compartments. These molecules transport endosomal muramyl dipeptides (MDPs), which are sensed by NOD2 in the cytoplasm (51). SLC15A4 is required for TLR7 and TLR9 responses in pDCs (52). It also mediates AP3-dependent endosomal trafficking required for TLR7 and TLR9 responses (53). Moreover, SLC15A4 serves as a scaffold protein by associating with TLR adaptor interacting with SLC15A4 on the lysosome (TASL) (54), which recruits IRFs to transmit signals from TLR7, TLR8, and TLR9. These molecules mediate the production of TLR-dependent type-I IFN. Interestingly, SLC15A4, IRF5 and TASL are all lupus-associated genes (55), which strongly suggest that type I IFN production by endosomal TLRs is activated in SLE.
1.4 Inflammatory Diseases Associated With Dysregulated Responses of Endosomal TLRs
1.4.1 Monogenic Diseases
Gain-of-function mutations in the TLR8 gene such as P432L, F494L, and G527D, cause neutropenia, infections, lymphoproliferation, and B cell deficiency (56). Although TLR8 is expressed in myeloid cells, T cell activation and B cell deficiency develop, probably because of the cell non-autonomous mechanisms. These clinical manifestations are not necessarily consistent with the phenotypes of TLR8 transgenic mice, in which TLR8 expression is driven by a human endogenous promoter (57). TLR8 transgenic mice exhibit severe inflammation in the pancreas, salivary glands, and joints. In contrast to human patients harboring gain-of-function mutations in the TLR8 gene, neither neutropenia nor B cell deficiency was observed. The TLR8 responses in TLR8 transgenic mice are distinct from those in humans.
Constitutive activation of TLR7 due to its gain-of-function mutation causes monogenic SLE in humans (58). The increase in B cell number depends on TLR7 expression. Because TLR7 is expressed not only in myeloid cells, but also in B cells, mutated TLR7 drives cell-autonomous B cell activation. A lupus-prone mouse strain, the Y-linked autoimmune accelerator strain, has an additional copy of the TLR7 gene that results in TLR7 hyperactivation, leading to lupus-like state (59, 60). Clinical manifestations in patients harboring gain-of-function mutations in TLR7 genes differ from those with TLR8 mutations and are ascribed to different expression in different immune cells. TLR7 is highly expressed in B cells and pDCs, whereas TLR8 is highly expressed in monocytes and macrophages.
The ACP5 gene encodes lysosomal acid phosphatase expressed in osteoclasts, macrophages, and DCs. Loss-of-function mutations in the ACP5 gene cause spondyloenchondrodysplasia with immune dysregulation, a disease characterized by skeletal dysplasia and neurologic and autoimmune manifestations (61). The detailed mechanisms underlying autoimmune manifestations remain unclear. ACP5 deficiency increases the level of hyperphosphorylated osteopontin, which is suggested to promote TLR9 responses in osteoclasts and macrophages.
1.4.2 Polygenic Diseases
SLE is an autoimmune disease characterized by autoantibody production and clinical manifestations affecting the skin, joints, kidneys, and the central nervous system (62). Causative autoimmune responses are driven by autoreactive B cells that produce autoantibodies against NA-associated autoantigens and cDCs and pDCs that produce proinflammatory cytokines and type I IFN, respectively (63, 64). In addition to these cells, monocytes/macrophages infiltrate the glomeruli and play pathologic roles in glomerular damage associated with SLE, independent of immune complex (IC) deposition (65–67). The TLR7 agonist imiquimod drives lupus nephritis in mice (68, 69), whereas the pathologies in the lupus-prone strain, New Zealand Black/New Zealand White F1 (NZBWF1) mice, is ameliorated by TLR7 chemical inhibitor or by anti-TLR7 monoclonal antibody (70, 71). The number of Ly6Clow patrolling monocytes TLR7-dependently increases in NZBWF1 mice (39). Interestingly, during monocyte maturation from Ly6Chi to Ly6Clow cells, TLR9 expression decreases with TLR7 expression unchanged (72). The IC-independent glomerular accumulation of Ly6Clow patrolling monocytes causes lupus nephritis in another lupus-prone mouse strain lacking the human SLE susceptibility gene, Tnip1 (67).
The TLR7-dependent increase in Ly6Clow monocytes/macrophages might be driven by self-derived RNAs. The 60 kDa Ro60 ribonucleoprotein, also known as the SSA/Ro antigen, is one of the most studied autoantigens associated with SLE or primary Sjögren syndrome. Because Alu retroelements, repetitive transposons, bind to Ro60 and activate TLR7 and TLR8 (73), the IC consisting of Ro60, Alu retroelements, and autoantibodies is formed in lupus-prone mice and internalized by autoreactive B cells or DCs via the BCR or FcR, respectively. Alu retroelements in the IC activate endosomal TLR7 or TLR8 to drive autoimmunity (74). TLR7 may also be activated by RNA from bacteria, which enter the circulation through the leaky gut (75). Notably, commensal orthologs of Ro60 might play a pathologic role in SLE (76).
Systemic sclerosis (SSc) is a multisystem life-threatening fibrosing disorder (77). Aberrant TLR8 expression in pDCs has been reported in patients with SSc (78). pDCs normally express only TLR7. Additional expression of TLR8 may promote autoimmune responses in SSc.
Author Contributions
KM wrote the manuscript. TS, RF, RS, SS, and YM made comments on the manuscript. All authors contributed to the article and approved the submitted version.
Funding
This work was supported in part by: Grant-in-Aid for Scientific Research (S and A) to KM (16H06388, 21H04800), (B) to S-IS (26293083), and (C) (16K08827) to TS; JST CREST (JPMJCR13M5, JPMJCR21E4) to TS and KM, respectively; Joint Research Project of the Institute of Medical Science at the University of Tokyo; and JSPS KAKENHI Grant Number JP 16H06276 (AdAMS).
Conflict of Interest
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as potential conflicts of interest.
Publisher’s Note
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Keywords: nuclease, nucleoside, autoimmune disease, endosome, toll-like receptor
Citation: Miyake K, Shibata T, Fukui R, Sato R, Saitoh S-I and Murakami Y (2022) Nucleic Acid Sensing by Toll-Like Receptors in the Endosomal Compartment. Front. Immunol. 13:941931. doi: 10.3389/fimmu.2022.941931
Received: 12 May 2022; Accepted: 27 May 2022;
Published: 23 June 2022.
Edited by:
Hang Yin, Tsinghua University, ChinaReviewed by:
Yoshinori Hirano, The University of Tokyo, JapanCopyright © 2022 Miyake, Shibata, Fukui, Sato, Saitoh and Murakami. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Kensuke Miyake, kmiyake@ims.u-tokyo.ac.jp