AUTHOR=Sun Tao , Zhang Xinru , Hou Chao , Yu Shujun , Zhang Yujing , Yu Zhuo , Kong Ling , Liu Changqing , Feng Lijie , Wang Dong , Ni Guohua 

TITLE=Cold Plasma Irradiation Attenuates Atopic Dermatitis via Enhancing HIF-1α-Induced MANF Transcription Expression

JOURNAL=Frontiers in Immunology

VOLUME=13

YEAR=2022

URL=https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2022.941219

DOI=10.3389/fimmu.2022.941219

ISSN=1664-3224

ABSTRACT=<p>Cold atmospheric plasma has been widely applied in medical treatment clinically, especially skin diseases. However, the mechanism of cold atmospheric plasma on the treatment of skin diseases is still undefined. In this study, dinitrofluorobenzene-induced atopic dermatitis mice model was constructed. Cold atmospheric plasma was able to decrease skin cells apoptosis, relieve skin inflammation, ER stress and oxidative stress caused by dinitrofluorobenzene stimulation, which was mediated by cold atmospheric plasma-induced MANF expression. In terms of mechanism, hypoxia-inducible factor-1α expression was increased intracellularly after cold atmospheric plasma treatment, which further bound to the promoter region of <italic>manf</italic> gene and enhanced MANF transcriptional expression. This study reveals that cold atmospheric plasma has a positive effect on atopic dermatitis treatment, also demonstrates the regulatory mechanism of cold atmospheric plasma on MANF expression <italic>via</italic> HIF-1α, which indicates the potential medical application of cold atmospheric plasma for atopic dermatitis treatment.</p>