AUTHOR=Royzman Dmytro , Andreev Darja , Stich Lena , Peckert-Maier Katrin , Wild Andreas B. , Zinser Elisabeth , Mühl-Zürbes Petra , Jones Evan , Adam Susanne , Frey Silke , Fuchs Maximilian , Kunz Meik , Bäuerle Tobias , Nagel Lisa , Schett Georg , Bozec Aline , Steinkasserer Alexander TITLE=The soluble CD83 protein prevents bone destruction by inhibiting the formation of osteoclasts and inducing resolution of inflammation in arthritis JOURNAL=Frontiers in Immunology VOLUME=13 YEAR=2022 URL=https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2022.936995 DOI=10.3389/fimmu.2022.936995 ISSN=1664-3224 ABSTRACT=
Here we show that soluble CD83 induces the resolution of inflammation in an antigen-induced arthritis (AIA) model. Joint swelling and the arthritis-related expression levels of IL-1β, IL-6, RANKL, MMP9, and OC-Stamp were strongly reduced, while Foxp3 was induced. In addition, we observed a significant inhibition of TRAP+ osteoclast formation, correlating with the reduced arthritic disease score. In contrast, cell-specific deletion of CD83 in human and murine precursor cells resulted in an enhanced formation of mature osteoclasts. RNA sequencing analyses, comparing sCD83- with mock treated cells, revealed a strong downregulation of osteoclastogenic factors, such as Oc-Stamp, Mmp9 and Nfatc1, Ctsk, and Trap. Concomitantly, transcripts typical for pro-resolving macrophages,