AUTHOR=Wang Jie , Huang Feifan , Zhao Jingjie , Huang Peng , Tan Junhua , Huang Meiying , Ma Ruiying , Xiao Yu , He Siyuan , Wang Zechen , Shen Jiajia , Lu Heming , Meng Lingzhang 

TITLE=Tumor-Infiltrated CD8+ T Cell 10-Gene Signature Related to Clear Cell Renal Cell Carcinoma Prognosis

JOURNAL=Frontiers in Immunology

VOLUME=13

YEAR=2022

URL=https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2022.930921

DOI=10.3389/fimmu.2022.930921

ISSN=1664-3224

ABSTRACT=<p>Clear cell renal cell carcinoma (ccRCC) usually affects multiple organs (e.g., bone and brain), and patient prognosis is usually poor. Although it is known that CD8+ T cell infiltration can potentially alleviate ccRCC progression, few studies have concentrated on the correlation between CD8+ T cell infiltration and ccRCC prognosis. In this study, ten genes expressed by infiltrated CD8+ T cells (i.e., <italic>AMD1, CCSER2, CIB1, DRAP1, HMGB2, HMGN1, NPIPB5, PTP4A2, RORA</italic>, and <italic>SAP18</italic>) were suggested as potential ccRCC prognostic biomarkers, by using next-generation sequencing (i.e. bulk sequencing and single-cell sequencing) of ccRCC, papillary renal cell carcinoma (papRCC), and control kidney biopsies. Specifically, we identified four genes (i.e., <italic>CCSER2, DRAP1, NPIPB5</italic>, and <italic>SAP18</italic>) as potential novel prognostic biomarkers for ccRCC. It is noteworthy that <italic>SAP18</italic> derived from CD8+ T cells negatively correlates to Atg7+ neutrophils in ccRCC, compared with papRCC, indicating a potential decreased neutrophil metabolic function in autophagy and fatty acids. This study elucidated the protective role of infiltrated CD8+ T cells in ccRCC and identified ten candidate genes related to an improved prognosis in patients with ccRCC.</p>