AUTHOR=Zhao Hai-chao , Chen Chang-zhou , Song Huang-qin , Wang Xiao-xiao , Zhang Lei , Zhao Hao-liang , He Jie-feng 

TITLE=Single-cell RNA Sequencing Analysis Reveals New Immune Disorder Complexities in Hypersplenism

JOURNAL=Frontiers in Immunology

VOLUME=13

YEAR=2022

URL=https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2022.921900

DOI=10.3389/fimmu.2022.921900

ISSN=1664-3224

ABSTRACT=<p>Hypersplenism (HS) is a concomitant symptom of liver or blood disease. Not only does the treatment of HS face challenges, but the transcriptome of individual cells is also unknown. Here, the transcriptional profiles of 43,037 cells from four HS tissues and one control tissue were generated by the single-cell RNA sequencing and nine major cell types, including T-cells, B-cells, NK cells, hematopoietic stem cells, neutrophil cells, mast cells, endothelial cells, erythrocytes, and dendritic cells were identified. Strikingly, the main features were the lack of <italic>CCL5</italic><sup>+</sup> B-cells in HS and the presence of <italic>SESN1</italic><sup>+</sup> B cells in HS with hepatocellular carcinoma (HS-HCC). In cell-cell interaction analysis, CD74-COPA and CD94-HLA-E in HS were found to be up-regulated. We further explored HS-specifically enriched genes (such as <italic>FKBP5</italic>, <italic>ADAR</italic>, and <italic>RPS4Y1</italic>) and found that <italic>FKBP5</italic> was highly expressed in HCC-HS, leading to immunosuppression. Taken together, this research provides new insights into the genetic characteristics of HS <italic>via</italic> comprehensive single-cell transcriptome analysis.</p>