AUTHOR=Lai Chih-Ho , Lin Tzu-Lung , Huang Mei-Zi , Li Shiao-Wen , Wu Hui-Yu , Chiu Ya-Fang , Yang Chia-Yu , Chiu Cheng-Hsun , Lai Hsin-Chih 

TITLE=Gut Commensal Parabacteroides goldsteinii MTS01 Alters Gut Microbiota Composition and Reduces Cholesterol to Mitigate Helicobacter pylori-Induced Pathogenesis

JOURNAL=Frontiers in Immunology

VOLUME=13

YEAR=2022

URL=https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2022.916848

DOI=10.3389/fimmu.2022.916848

ISSN=1664-3224

ABSTRACT=<p><italic>Helicobacter pylori</italic> infection is closely associated with various gastrointestinal diseases and poses a serious threat to human health owing to its increasing antimicrobial resistance. <italic>H. pylori</italic> possesses two major virulence factors, vacuolating cytotoxin A (VacA) and cytotoxin-associated gene A (CagA), which are involved in its pathogenesis. Probiotics have recently been used to eradicate <italic>H. pylori</italic> infection and reduce the adverse effects of antibiotic-based therapies. <italic>Parabacteroides goldsteinii</italic> MTS01 is a novel next-generation probiotic (NGP) with activities that can alleviate specific diseases by altering the gut microbiota. However, the mechanism by which <italic>P. goldsteinii</italic> MTS01 exerts its probiotic effects against <italic>H. pylori</italic> infection remains unclear. Our results showed that administration of <italic>P. goldsteinii</italic> MTS01 to <italic>H. pylori</italic>-infected model mice altered the composition of the gut microbiota and significantly reduced serum cholesterol levels, which mitigated <italic>H. pylori</italic>-induced gastric inflammation. In addition, the pathogenic effects of <italic>H. pylori</italic> VacA and CagA on gastric epithelial cells were markedly abrogated by treatment with <italic>P. goldsteinii</italic> MTS01. These results indicate that <italic>P. goldsteinii</italic> MTS01 can modulate gut microbiota composition and has anti-virulence factor functions, and thus could be developed as a novel functional probiotic for reducing <italic>H. pylori</italic>-induced pathogenesis.</p>