AUTHOR=Zhou Xuezhi , Rong Rong , Xiong Siqi , Song Weitao , Ji Dan , Xia Xiaobo 

TITLE=Integrated analysis to reveal potential therapeutic targets and prognostic biomarkers of skin cutaneous melanoma

JOURNAL=Frontiers in Immunology

VOLUME=13

YEAR=2022

URL=https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2022.914108

DOI=10.3389/fimmu.2022.914108

ISSN=1664-3224

ABSTRACT=<p>Skin cutaneous melanoma (SKCM) is a malignant tumor with high mortality rate in human, and its occurrence and development are jointly regulated by genes and the environment. However, the specific pathogenesis of SKCM is not completely understood. In recent years, an increasing number of studies have reported the important role of competing endogenous RNA (ceRNA) regulatory networks in various tumors; however, the complexity and specific biological effects of the ceRNA regulatory network of SKCM remain unclear. In the present study, we obtained a ceRNA regulatory network of long non-coding RNAs, microRNAs, and mRNAs related to the phosphatase and tensin homolog (<italic>PTEN</italic>) in SKCM and identified the potential diagnostic and prognostic markers related to SKCM. We extracted the above three types of RNA involved in SKCM from The Cancer Genome Atlas database. Through bioinformatics analysis, the <italic>OIP5-AS1</italic>-hsa-miR-186-5p/hsa-miR-616-3p/hsa-miR-135a-5p/hsa-miR-23b-3p/hsa-miR-374b-5p-<italic>PTPRC</italic>/<italic>IL7R</italic>/<italic>CD69</italic> and <italic>MALAT1</italic>-hsa-miR-135a-5p/hsa-miR-23b-3p/hsa-miR-374b-5p-<italic>IL7R</italic>/<italic>CD69</italic> ceRNA networks were found to be related to the prognosis of SKCM. Finally, we determined the <italic>OIP5-AS1-PTPRC</italic>/<italic>IL7R</italic>/<italic>CD69</italic> and <italic>MALAT1-IL7R</italic>/<italic>CD69</italic> axes in ceRNA as a clinical prognostic model using correlation and Cox regression analyses. Additionally, we explored the possible role of these two axes in affecting gene expression and immune microenvironment changes and the occurrence and development of SKCM through methylation and immune infiltration analyses. In summary, the ceRNA-based <italic>OIP5-AS1-PTPRC</italic>/<italic>IL7R</italic>/<italic>CD69</italic> and <italic>MALAT1-IL7R</italic>/<italic>CD69</italic> axes may be a novel and important approach for the diagnosis and prognosis of SKCM.</p>