Bingxia Chen
Zemin Han2- 1Guangzhou Women and Children’s Medical Center, Guangzhou Medical University, Guangzhou, China
- 2Nanfang Hospital, Southern Medical University, Guangzhou, China
Traditional observational studies have indicated a link between specific food intakes and inflammatory bowel disease (IBD), but the nature of such links remains unknown. We sought to assess the potential causal relationship between food intakes and IBD risk using Mendelian randomization methods. This study used summary statistics data from large-scale genome-wide association studies (GWAS) on food intakes, Crohn’s disease (CD), and ulcerative colitis (UC). In the primary analysis, we used the inverse variance-weighted method to determine whether specific food was causal for CD and UC. In addition, we also ran four other Mendelian randomization methods, including MR Egger, weighted median, maximum likelihood, and weighted mode as a complement. The primary analysis showed that high consumption of poultry (OR, 3.696; 95% CI, 1.056–12.937; p = 0.041) and cereal (OR, 2.449; 95% CI, 1.094–5.482; p = 0.029) had a significant causal association with CD, while high oily fish intake level was found to be statistically significantly associated with the risk of UC (OR, 1.482; 95% CI, 1.002–2.194; p = 0.049). This MR study provides evidence of a potential causal link between certain food intake and CD and UC.
Introduction
Inflammatory bowel disease (IBD), including Crohn’s disease (CD) and ulcerative colitis (UC), is a multifactorial disease characterized by a deregulated immune response to environmental and microbial components on a genetic susceptibility background (1). While several environmental factors participate in the pathogenesis and progression of IBD, the role of diet has attracted considerable attention. Although the exact mechanism remains uncertain, it has been proposed that certain food intake may modify the risk of IBD through its impact on host immunity system, gut barrier, and gut microbiome, all of which are critical factors in IBD pathogenesis (2–6). Many food risk factors have been established to be associated with IBD pathogenesis, especially the components of a Western diet, which is known to be high in fat, n-6 polyunsaturated fatty acids (PUFAs), and red and processed meat, and low in fruits and vegetables (7).
While a few studies have identified some food risk factors for IBD progression, insufficient evidence supports their causal roles in IBD incidence. Some cross-sectional studies were conducted to determine the diet responsible for IBD incidence. These observations, however, might be confounded by unidentified factors and therefore contradict the causality of the associations. RCTs are the gold standard for determining a causal relationship (8, 9). However, due to ethical constraints, an RCT is difficult to implement in most cases. Mendelian randomization (MR) analysis can help overcome these limitations. In MR analysis, genetic variants such as single-nucleotide polymorphisms (SNPs) are used as instrumental variables (IVs) to estimate the causal associations between an exposure and an outcome (10). Since genetic variation is inherited from parents and remains unchanged after birth, the association between genetic variation and outcome is reasonable. MR analysis relies on three critical assumptions: (i) IVs are strongly associated with exposure; (ii) IVs should be independent of confounders of exposure and outcome; and (iii) IV–outcome association is only mediated via exposure (10).
Understanding the exact role of foods in IBD risk may be helpful to develop more effective prevention, prediction, and treatment strategies for essential conditions. Therefore, we applied the MR method to analyze the causal relationships between food intakes and two IBD subtypes, CD and UC.
Methods
Data sources
Genome-wide association studies of food intakes
A flowchart describes the study design briefly (Figure 1). For summary statistics for food intakes, we used data from the UK Biobank (UKB). The UKB project is a large, prospective cohort study with about 500,000 participants from the United Kingdom (11).
Figure 1 Flowchart of MR analysis in this study. IBD, inflammatory bowel disease; CD, Crohn’s disease; UC, ulcerative colitis; MAF, minor allele frequency; IVs, instrumental variables; SNPs, single-nucleotide polymorphisms; MR analysis, Mendelian randomization analysis.
Genome-wide association studies of CD and UC
For CD and UC, we used publicly available meta-analysis GWAS (12). GWAS of CD (ebi-a-GCST004132) included 12,194 cases and 28,072 control subjects, and GWAS of UC (ebi-a-GCST004133) included 12,366 cases and 33,609 control subjects.
Genetic IV selection
We selected eligible genetic IVs using a series of quality control criteria based on the GWAS summary food data. First, we used independent genetic variants significantly associated (p < 5 × 10−8) for each instrument with each exposure. Then, we performed the clumping procedure with R2 < 0.001 and a window size >10,000 kb to avoid linkage disequilibrium (LD) (13). Third, we eliminated SNPs with a minor allele frequency (MAF) of less than 0.01. Fourth, to prevent potential pleiotropic effects for the instruments, we used Phenoscanner, a database that includes genotype–phenotype associations (14). We searched for each SNP included as an instrument in our analysis and removed SNPs associated with IBD, CD, or UC. SNP harmonization was also performed to rectify the orientation of the alleles (15).
Evaluation of the strength of the genetic instruments
To assess the strength of genetic instruments for each food intake, we calculated the proportion of variance explained (R2) and F statistics for all SNPs. IVs (F statistics < 10) were considered weak instruments, and the exposure would be excluded from MR analysis (16).
Pleiotropy, heterogeneity, and sensitivity analysis
MR Egger regression was used to assess the possibility of horizontal pleiotropy, as indicated by the intercept (17). In the presence of pleiotropy (p ≤ 0.05), MR Pleiotropy REsidual Sum and Outlier (MR-PRESSO) test was conducted using MR-PRESSO package in R, and SNP with the smallest pleiotropy p-value was removed (18). In addition, we used the inverse-variance weighted (IVW) approach and MR Egger regression to identify heterogeneity, which was quantified using Cochran’s Q statistic. In addition, we conducted the leave-one-out analysis to identify the stability of results.
MR analysis
In this study, we used IVW two-sample MR as our primary analysis (19) to estimate the causal effects of exposures on the risk of CD and UC. The IVW method assumes that all variants are valid IVs, providing the most precise results. In addition, we ran MR Egger, weighted median, maximum likelihood, and weighted mode as a complement. The MR analysis was carried out in R using TwoSampleMR and MendelianRandomization packages (20).
Results
Overall, 17 kinds of food intake exposures were included in our study, excluding those without effective IVs (i.e., milk intake). The number of SNPs for each food intake ranged from 6 to 48 after a series of quality control steps (Tables 1, 2). The F statistic values were more than the empirical threshold of 10, suggesting that all SNPs had sufficient validity.
Table 1 Summary of modifiable risk factors for CD.
Table 2 Summary of modifiable risk factors for UC.
MR estimates of different methods are presented in Tables 3, 4. In the primary IVW MR analysis, two causal associations from 17 food intakes to CD were identified, while only one causal association was observed for UC. As shown in Figures 2, 3, We found evidence that increased consumption of poultry was associated with a higher risk of CD (OR, 3.696; 95% CI, 1.056–12.937; p = 0.041) but was not associated with UC (OR, 0.633; 95% CI, 0.114–3.505; p = 0.600). Higher cereal intake level also increased CD risk (OR, 2.449; 95% CI, 1.094–5.482; p = 0.029). On the contrary, genetically predicted oily fish intake level was statistically significantly associated with the risk of UC (OR, 1.482; 95% CI, 1.002–2.194; p = 0.049) but not with CD (OR, 1.010; 95% CI, 0.603–1.692; p = 0.969). In addition to the above causal associations identified by the IVW test, several intriguing results were identified by other MR tests, including cheese intake based on maximum likelihood method (OR, 0.671; 95% CI, 0.474–0.950; p = 0.024) and processed meat intake based on the weighted median method (OR, 0.360; 95% CI, 0.136–0.952; p = 0.040), both of which were suggested to be associated with a decreased risk of CD (Table 3). Conversely, bread intake based on the weighted median method (OR, 0.332; 95% CI, 0.146–0.752; p = 0.008) and dried fruit intake based on the MR Egger method (OR, 0.029; 95% CI, 0.001–0.603; p = 0.030) were suggested to be associated with a decreased UC risk (Table 4).
Table 3 Results of the MR study testing causal association between risk factors and CD.
Table 4 Results of the MR study testing causal association between risk factors and UC.
Figure 2 The causal effect of food risk factors on CD based on the IVW method. IVW, inverse-variance weighted; CD, Crohn’s disease.
Figure 3 The causal effect of food risk factors on UC based on the IVW method. Abbreviations: IVW, inverse-variance weighted; UC, ulcerative colitis.
The scatter plots, forest plots, funnel plots, and leave-one-out plots for CD and UC are displayed in Figures S1A–Q, S2A–Q, S3A–Q, S4A–Q, S5A–Q, S6A–Q, S7A–Q, and S8A–Q.
Discussion
The etiology of IBD is complex, involving immune imbalance, like dysregulated IL-23/Th17, alteration of microbiome, and infection (1, 21). There is mounting evidence that certain foods may increase or decrease IBD risk in susceptible individuals (2, 4, 22–26). MR analysis was conducted to evaluate the potential causality between food intakes and IBD in this study, which uses random allocation of alleles to replicate the randomization process in double-blind clinical trials. Using large-scale summary statistics from food intake GWAS and CD, UC GWAS, we identified specific food intake that might be causally associated with CD and UC risk.
High red meat intake is one of the features of the Western diet, which is believed to be a risk factor for IBD. Peters et al. found that the “carnivorous” dietary pattern, which consists of high consumption of red meat, poultry, and processed meat, was associated with UC development (OR: 1.11, 95% CI, 1.01–1.22, p = 0.024) but not with CD (OR: 0.99, 95% CI, 0.86–1.33, p = 0.853) in a prospective population-based cohort (22). Animal studies have indicated that iron, sulfur, and fats are risk factors for colitis and ileitis, which are found in high concentrations in meats (27, 28). Hydrogen sulfide (H2S) has been demonstrated to have detrimental inflammatory effects on the colon (29). However, our MR study did not find any association between red meat intake (beef intake, lamb intake, and pork intake) and IBD risk but discovered a causal association between poultry intake and CD risk. Based on another large, multinational, prospective cohort study involving 116,087 participants from 21 countries, intakes of red meat and white meat were not associated with incident IBD (30).
Despite previous evidence from human studies supporting a possible link between processed meat consumption and IBD, the conclusion is inconsistent. It was reported that higher processed meat consumption was associated with a higher risk of IBD in Narula et al.’s study (30). However, in another prospective cohort study of three national cohorts of American health professionals, which included 245,112 participants, the author found that although higher ultra-processed food intake was associated with an increased risk of incident CD, meat products were not related to the risk of CD (23). In contrast, ultra-processed bread and other processed foods showed positive associations with CD risk (23). The conclusion is controversial because when people cut back on processed meat in their diet, they must replace it with something else. Participants in different studies may replace processed meat with different foods, affecting CD risk. Through MR analysis, our study suggested that there might be an inverse causal relationship between processed meat consumption and CD risk.
Another feature of the Western diet is a low intake of fruits and vegetables. Diets high in fruits and vegetables were found to be inversely related to CD in a large prospective cohort study (26). In addition, a nested matched case–control study using a large European Prospective Investigation into Cancer and Nutrition (EPIC) prospective database found that low vegetable intake was associated with an increased risk of UC (31). One explanation of fruit and vegetable’s beneficial role in IBD is high fiber. Because fiber reduces intestinal transit times, potential toxic exposures have less time to contact the intestinal wall. In addition, fiber can be converted into short-chain fatty acids (SCFAs), such as butyrate, which enhances mucus and antimicrobial peptide secretion, and modulates intestinal inflammation by suppressing pro-inflammatory mediators (32, 33). Fiber may also help maintain the intestinal barrier by reducing pathogen translocation across Peyer’s patches and colonic lymphoid follicles (34). Despite a few researchers reporting some protective effects and all of these proposed protective mechanisms, studies of fiber and disease onset and clinical relapse of IBD did not find any consistent effects (4, 35, 36). Narula and his colleagues reported that intake of fruit and vegetables was not associated with incident IBD (30). Based on the MR Egger method, dried fruit intake might be associated with a decreased UC risk in our study, while no relationship was found between fruit or vegetable intake and CD in our study.
Our MR analysis also indicated that high oily fish intake level might increase the risk of UC. Interestingly, in a meta-analysis, increased fish intake was a protective factor for CD in Western countries, but a risk factor for UC in Eastern countries (37). In a prospective cohort study consisting of 67581 women living in France, high consumption of meat or fish but not dairy products was found to be associated with IBD risk among sources of animal protein (38).
In addition, we found that cereal intake may increase the risk of CD and bread intake might decrease the risk of UC. In Jakosen et al.’s study, whole meal bread consumption was found to be a protective factor for CD, while white bread consumption and cereal cornflake type were found to be risk factors (33).
Dairy products, including milk, yogurt, and cheese, are common components of a Western diet. In the EPIC cohort, the researchers found that dairy product consumption may be associated with a decreased risk of CD (39). Our study also suggested that there might be a negative association between cheese and CD risk.
Several studies have demonstrated that alcohol modulates the immune system in a dose- and time-dependent manner (40, 41). However, in a recent Mendelian randomization analysis conducted by Xia Jiang et al., alcohol intake did not show a causal role in IBD risk (42). In addition, our study did not find any association between alcohol intake and IBD either.
Our research has several significant strengths, out of which the dominant one is the MR design, which is suitable for causal inference. Given the numerous challenges of designing and carrying out RCTs in IBD, an MR study could provide important insights into the associations between specific dietary components and the risk of developing IBD. Furthermore, the food intake factors included in our research, such as processed meat intake, have not previously been investigated in an MR setting. As a result, this study could serve as a model for future research into the relationship between food intake and disease risk.
However, some limitations in this MR study should be observed. First, food intake GWAS remains in its infancy in sample size and could bring compromised statistical power. The limited IV numbers weaken the proportion of phenotypic variance explained. Therefore, the null findings for some associations do not necessarily indicate that food intake has no effect. Second, we only included 17 kinds of food, as other food intakes (i.e., milk intake) do not have enough effective IVs. In addition, one thing should be noted: although only single food items were investigated in our study, these elements may act synergistically or antagonistically as part of a habitual diet (43). The dietary patterns should be studied in MR research to assess their role in CD and UC risk.
In conclusion, we thoroughly examined the potential causal relationship between food intakes and CD and UC. Two types of food intake (poultry intake and cereal intake) were found to increase the risk of CD, and high oily fish intake was associated with UC risk. More research is needed in the future to determine the exact causal relationship and mechanism underlying specific food intakes and IBD.
Data availability statement
The original contributions presented in the study are included in the article/Supplementary Material. Further inquiries can be directed to the corresponding author.
Author contributions
All authors listed have made a substantial, direct, and intellectual contribution to the work, and approved it for publication.
Funding
This work was supported by Research foundation of Guangzhou Women and Children’s Medical Center for Clinical Doctor (grant number 1600111).
Conflict of interest
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Publisher’s note
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.
Supplementary material
The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fimmu.2022.911631/full#supplementary-material
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Keywords: Mendelian randomization analysis, causal effects, food intakes, risk factors, inflammatory bowel disease
Citation: Chen B, Han Z and Geng L (2022) Mendelian randomization analysis reveals causal effects of food intakes on inflammatory bowel disease risk. Front. Immunol. 13:911631. doi: 10.3389/fimmu.2022.911631
Received: 02 April 2022; Accepted: 24 August 2022;
Published: 22 September 2022.
Edited by:
Amber Farooqui, University of Toronto, CanadaReviewed by:
Marcos Edgar Herkenhoff, University of São Paulo, BrazilVanessa Fernandes Rodrigues, Department of Biochemistry and Immunology, University of São Paulo, Brazil
Giuseppe Murdaca, University of Genoa, Italy
Zhixing He, Zhejiang Chinese Medical University, China
Copyright © 2022 Chen, Han and Geng. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Bingxia Chen, 2589935838@qq.com