David Arranz-Solís
Jeroen P. J. Saeij- Pathology, Microbiology and Immunology department, School of Veterinary Medicine, University of California Davis, Davis, CA, United States
Toxoplasmosis is a worldwide disease affecting all warm-blooded animals, including humans. Vaccination strategies aimed at inducing an efficient immune response while preventing transmission have been attempted in the past. While many different approaches can partially protect immunized animals against subsequent infections, full and lasting protection is rarely attained and only with live-attenuated vaccines. In addition, vaccines based on mutant strains that are deficient in forming the chronic phase of the parasite (such as Toxovax™) cannot be extensively used due to their zoonotic potential and the possibility of reversion to virulent phenotypes. An increasing number of studies using emerging genetic-engineering tools have been conducted to design novel vaccines based on recombinant proteins, DNA or delivery systems such as nanoparticles. However, these are usually less efficient due to their antigenic simplicity. In this perspective article we discuss potential target genes and novel strategies to generate live-attenuated long-lasting vaccines based on tissue cysts and oocysts, which are the environmentally resistant chronic forms of Toxoplasma. By selectively disrupting genes important for parasite dissemination, cyst formation and/or sporozoite invasion, alone or in combination, a vaccine based on a live-attenuated strain that elicits a protective immune response while preventing the transmission of Toxoplasma could be created. Finally, further improvements of protocols to generate Toxoplasma sexual stages in vitro might lead to the production of oocysts from such a strain without the need for using mice or cats.
Introduction
The phylum Apicomplexa comprises a large group of protozoan parasites, many of which cause disease in humans and livestock, such as Plasmodium spp., Toxoplasma gondii, Neospora spp. or Eimeria spp (1). Toxoplasmosis is a worldwide zoonotic disease and one of the leading causes of foodborne illness in the USA. It can affect virtually all mammals and birds, including approximately one third of humans (2). Although the majority of infected healthy individuals are asymptomatic, this disease can cause neonatal mortality, abortions and a wide variety of neurological symptoms, especially in immunocompromised or congenitally infected patients (3, 4). In addition, toxoplasmosis causes important economic losses in the livestock sector related to reproductive failure, mainly in sheep and goats (5).
In intermediate hosts, which include any warm-blooded animal, infection can occur after ingestion of tissue cysts present in meat or viscera from infected animals, or oocysts shed in the feces of an infected feline (definitive hosts that can also act as intermediate hosts). Upon ingestion, these orally infectious forms readily differentiate into tachyzoites, the fast-replicating stage responsible for proliferation and dissemination throughout the host and also for the clinical symptoms during the acute phase of the disease. After eliciting an immune response, tachyzoites transform into bradyzoites, the slow-replicating stage that forms tissue cysts. These are found mainly in the brain and muscle, remaining intact for long periods, possibly throughout the life of the host (6). When a cat ingests tissues from an infected animal, bradyzoites invade intestinal epithelial cells and differentiate into merozoites. After multiple replication cycles, those merozoites eventually differentiate into micro- (male) and macro- (female) gametes that upon fusion will ultimately form hundreds of millions of oocysts, which are shed unsporulated within cat feces (7). Once in the environment, and under favorable conditions of humidity, aeration and temperature, sporulation takes place and another asexual form of the parasite, called sporozoites, develops within oocysts. The mature (or sporulated) Toxoplasma oocyst has in its final form 2 sporocysts containing 4 sporozoites each. Oocysts are highly stable in the environment, extremely resistant to inactivation procedures, and exceptionally infectious to intermediate hosts, as even a single oocyst is capable of eliciting the infection. The oocyst and sporocyst walls provide an important protective barrier and likely protect the sporozoites from environmental stressors (8). For example, many studies have described that Toxoplasma oocysts can withstand freezing conditions for weeks and temperatures above 50 °C for several minutes (9–13). Similarly, many chemicals and common disinfectants, even strong solutions such as household bleach, ethanol or formalin, among others, are not able to inactivate oocysts (11, 14–18).
Toxoplasmosis in Cats
Toxoplasma infections in cats are typically subclinical. Nevertheless, congenitally infected kittens are the most likely to have symptoms, although healthy adult cats may also be affected (7, 19). The most common clinical signs of toxoplasmosis in cats include fever, diarrhea, or other severe signs such as pneumonia, ocular disease and, importantly, neurological signs. This concern is not to be disregarded, as the worldwide estimated seroprevalence for Toxoplasma in domestic cats (Felis catus) is 35–40%, being even higher in wild felids (~60-65%) (20, 21). In the USA, local seroprevalence studies have reported figures ranging from 16% to 43% (22, 23). However, these Toxoplasma seroprevalence rates might be overestimated as these studies employed serological assays with total Toxoplasma lysates as antigens, which could cross-react with other closely related parasites such as Cystoisospora spp., Hammondia spp. or Sarcocystis spp (24). Hence, future approaches using more specific antigens, such as recombinant proteins or peptides, could provide more accurate estimates (25, 26).
Although it is commonly assumed that cats only shed oocysts the first time they get infected and only for a short period of time, a growing body of evidence suggests that cats might shed oocysts more than once if they become immunosuppressed (27, 28), infected with other coccidian parasites such as Cystoisospora (29) or with heterologous Toxoplasma strains (30, 31). This, together with the extraordinary infectivity and durability of oocysts, supports the idea that cats play a critical role in the epidemiology of toxoplasmosis. Therefore, if cats could be vaccinated so they no longer shed infectious oocysts, this would drastically reduce the exposure of humans and animals. Indeed, a recent report evaluated the effects of a hypothetical cat vaccine on reducing the presence of Toxoplasma oocysts in the environment and its implication in human infections (32). Although it was deemed unfeasible to obtain a complete elimination of Toxoplasma oocyst-originated transmission in large populations, vaccinating cats could still significantly decrease the presence of oocysts in the environment and thus the probability of humans getting infected through contaminated food or water. In addition, it would also decrease the percentage of animals infected and therefore the economic losses and presence of tissue cysts in their meat, which would in turn lessen human infection through consumption of animal products.
Vaccines in Animals
Currently, there is only one Toxoplasma vaccine commercially available, and it is only authorized for use on sheep (33). This live attenuated vaccine (Toxovax™) is based on tachyzoites from the S48 strain, which is unable to form cysts or oocysts due to its exceptionally prolonged in vitro manipulation (34). Even though this vaccine can partially protect immunized sheep against abortion, it presents critical shortcomings in terms of safety, production and stability. Since the genetic basis for its attenuation is unknown, a possible reversion to virulence cannot be dismissed. Moreover, the short viability of tachyzoites in an extracellular environment greatly hinders its production and maintenance in the long term. When used in cats, the S48 strain elicited a strong immune response, while cats did not produce oocysts (35). Similarly, the chemically-induced mutant strain T-263, which lost its ability to form oocysts (36), was also tested in cats and shown to prevent oocyst shedding after subsequent challenges (37, 38). This deficiency was recently ascribed to either a deficient fertilization or a blockage of oocyst wall formation, as schizonts and gamonts were observed in the intestine, albeit without generation of oocysts (39). However, akin to the “cyst-less” S48 strain, the gene(s) affected by the chemical mutagenesis of the “oocyst-less” T-263 strain are not known (36). It seems that to achieve immunity to oocyst shedding, it is needed that bradyzoites invade intestinal epithelial cells and convert to merozoites, as trials with T-263 tachyzoites directly delivered into the duodenum of cats did not confer protection against subsequent infections, despite eliciting a marked antibody response. Conversely, oral administration of cysts or bradyzoites prevented oocyst shedding after a heterologous challenge (38). In another field trial, resident cats from a pig farm in Illinois, USA, were orally administered with frozen T-263 bradyzoites and a decreased seroprevalence in the farmed pigs was observed in the following 3 years, suggesting a lower oocyst environmental contamination (40). Other vaccination trials in cats with mutant Toxoplasma strains have been attempted in the past, most of which elicited protection against subsequent infections (35). For instance, vaccination of cats with the non-persistent and temperature-sensitive TS-4 RH strain (36) did not induce oocyst shedding (41). Similarly, a Beverly strain that was modified by irradiation treatments was able to partially prevent cats from shedding oocysts (42). However, akin to the S48 and T-263 strains, the exact gene modifications of these mutant strains are not known, thus rendering them not reliable for its usage in animals, especially those with the virulent RH background.
New Strategies for Toxoplasma Vaccination
If a parasite similar to S48 or T-263 unable to generate orally infectious forms (tissue cysts and/or oocysts) could be engineered with alterations in known genes, the safety problem could be partially solved. For example, in a recent study, a mutant strain deficient in the microgamete gene HAP2 was used to infect cats, and only a very reduced number of misshapen oocysts that failed to sporulate were produced (43). Although vaccination of cats with this strain prevented oocyst shedding after a subsequent challenge with the CZ strain, it did not preclude systemic dissemination of the parasite and cyst formation in their brains (43). Another example of a mutant strain for which the gene modification is known that was tested in cats is the MIC1-3 KO strain (44). Nevertheless, this mutant parasite from a type I background was not able to prevent cats from shedding oocysts when tachyzoites were used either subcutaneously or by oral route, a fact that reinforces the aforementioned notion that bradyzoite invasion of the intestinal cells is needed to confer protection against oocyst formation in vaccinated cats. Regardless, and similar to the S48 or T-263 strain, the production of these vaccines would face similar drawbacks in terms of stability, as tachyzoites would be needed to infect mice and obtain tissue cysts for oral vaccination. By contrast, vaccines based on oocysts would allow long-term conservation of the formulations on account of their durability and stability, facilitating production and maintenance. In addition, these oocysts could have the potential to be devised as a vehicle to deliver or express antigenic proteins from other parasites (e.g., Neospora caninum to protect dogs from shedding Neospora oocysts). Because bradyzoites are critical components for the transmission of infection, the use of a vaccine capable of inducing a protective immune response while preventing conversion to the bradyzoite stage would represent an ideal approach to reduce the presence of tissue cysts in the meat of infected animals, decreasing in turn human exposure. Therefore, the development of a novel vaccine consisting of Toxoplasma oocysts from a strain that can no longer convert into bradyzoites in the intermediate hosts could serve as a valuable tool. However, there is a challenge to generate the oocysts from a bradyzoite-deficient mutant strain, since a parasite is needed that can complete the sexual cycle in cats but from which the oocysts can no longer convert into tissue cysts upon ingestion by intermediate hosts.
By using the CRISPR/Cas9 technology, a strain defective in genes coding for proteins involved in tissue cyst formation can be obtained. Notwithstanding, this defect should only start once the parasites are in the cat intestine. To accomplish this, Cas9 could be expressed from the promoter of a Toxoplasma gene that is only expressed in the intestinal stages, which would allow disruption of genes essential for tissue cyst formation only when Toxoplasma undergoes the sexual cycle in cats. Different strategies, such as having the bradyzoite genes of interest flanked by LoxP sites and the CRE recombinase expression driven by specific intestinal-stage promoters could also be devised to attain the same goal. A number of genes have been shown to be active only in the intestine of the cat (43, 45). Among them, the RNA expression of two SRSs (SAG1-Related Surface) proteins, SRS22B and SRS22H, as well as the megakaryocyte stimulating factor (MSF), was highly upregulated in Toxoplasma cat intestinal stages compared to bradyzoites. Hence, by inserting the promoters of these genes upstream of the Cas9 (or CRE) coding sequence, the endonuclease (or recombinase) should be only expressed when the parasites convert into merozoites in the intestine of cats, while it should not in tachyzoites or bradyzoites.
Different research groups have identified molecular mediators that regulate the conversion of tachyzoites to bradyzoites and cyst formation. These include the specific cyst wall glycoprotein (CST1), which is a structural component of the cyst wall (46), the nucleotide-sugar transporter 1 (NST1), necessary for the glycosylation of the cyst wall (47), or the Apetala-2 transcription factor AP2XI-4, which is important for bradyzoite gene expression during conversion and cyst formation (48). Nevertheless, the most remarkable advancement in the understanding of the tachyzoite-to-bradyzoite conversion was recently made by Waldman et al., when the master regulator of bradyzoite formation was described (49). The Bradyzoite-Formation Deficient 1 (BFD1) protein was shown to be essential for bradyzoite conversion: its ablation rendered parasites unable to form tissue cysts both in vitro and in vivo, while its conditional overexpression is sufficient to induce differentiation (49).
Notwithstanding, when Cas9 is activated in the intestine of the cat, and in the absence of a selection process as it would happen in vitro, it is likely that not every single parasite will get the targeted genes of interest disrupted, as the double strand breaks can be repaired with small indels and retain the function of the gene. Because of this, introducing several gRNAs for different genes will increase the chance of having at least one or more of the targeted genes disrupted in each parasite, thus decreasing the likelihood of having intact or wild-type parasites after going through a cat. To overcome this possibility other approaches can also be adopted. For instance, targeting genes important for the dissemination/migration of the parasite, such as ROP17 (50) or the recently described TgWIP (51), would also considerably hinder the ability of Toxoplasma to reach the brain where the majority of cysts are formed. Likewise, if a gene that is important for the in vivo fitness of the parasite, such as the dense granule Myc regulation protein (MYR)1 (52), is targeted, it would add additional layers to ensure parasites do not form tissue cysts. By making a parasite strain defective in one or more of the aforementioned genes, it is likely that viable tissue cysts are no longer formed, while its in vitro fitness remains unaltered. If the generation of oocysts from this mutant strain could be engineered, intermediate hosts could be orally vaccinated. In this case, sporozoites would invade intestinal cells, and eventually convert into susceptible tachyzoites with compromised dissemination and virulence, and unable to differentiate into bradyzoites. Therefore, and akin to a natural infection, these oocysts should elicit a protective mucosal and systemic immune response. However, as opposed to a natural infection where bradyzoites and tissue cyst would be formed, parasites will be eventually eliminated by the immune response without the possibility to form orally infectious tissue cysts.
Although oocysts are extremely resistant and long-lasting, and a single cat would be sufficient to produce enough oocysts (hundreds of millions) for a theoretical large-scale vaccination test, growing ethical concerns with animal experimentation warrants future endeavors to find alternative methods to produce these oocysts. A recent breakthrough study by Di Genova et al. showed that the natural lack of the enzyme Δ-6 desaturase in felines, which does not occur in any other mammal, causes accumulation of linoleic acid in their intestine, cueing Toxoplasma to initiate its sexual reproduction (53). By exploiting this feline’s unique trait, they made it possible for the first time to produce Toxoplasma sexual stages and oocysts in vitro by culturing cat and mouse intestinal organoids infected with Toxoplasma in the presence of linoleic acid and a Δ-6 desaturase inhibitor. Moreover, the administration of these two compounds in mice orally infected with tissue cysts was sufficient to trigger oocyst shedding in their feces. However, both the yields and sporulation capacity of the oocysts obtained by these methods showed important deficiencies compared to those naturally produced in cats (53). Another recent discovery made by Farhat et al. (54) described the ATPase microrchidia protein (MORC), which blocks gene accessibility by its association with the histone deacetylase HDAC3 and several AP2 transcription factors. By acting as a repressor, it has been suggested that MORC could be a master regulator of developmental directionality, blocking the expression of non-tachyzoite genes. Indeed, depletion of MORC triggered bradyzoite conversion and the expression of several intestinal-stage specific genes, including genes encoding macrogamete and microgamete, oocyst wall, and sporozoite-specific proteins, among others (54). Therefore, MORC could possibly be exploited to induce sexual development without the need for experimental infections in cats and directly from tachyzoites. Finally, a novel avenue that could help to improve the in vitro production of oocysts could be the recently described micro-physiological system of intestinal tissue described by Humayun et al. (55). By using a novel micromolding technique to generate hollow structures, artificial tubes of intestine can be engineered, recapitulating the lumen geometries of the gut (55). If this system were to be combined with either, or both, of the discoveries mentioned above, the culture of Toxoplasma intestinal stages in vitro mirroring natural conditions could be significantly improved. In summary, although a refinement of these models is needed, they lead the way towards a future approach where a fully developed and standardized system can be broadly used to produce oocysts with similar features to those from cats without having to resort to in vivo assays.
Vaccines in the Definitive Host, the Cat
In contrast to intermediate hosts, to which oocysts are extremely infective, cats are much more likely to become infected, and subsequently shed oocysts, following ingestion of tissue cysts rather than tachyzoites or oocysts (56, 57). Indeed, the ingestion of one bradyzoite is usually sufficient to induce feline infection and oocyst shedding within the first week post-infection, whereas a feline usually needs to ingest 1000 oocysts to develop a less efficient infection that will lead to a decreased and delayed shedding of oocysts (56, 57). This difference is probably due to the fact that following the ingestion of tissue cysts, some bradyzoites convert to tachyzoites and some to schizonts (merozoites), which replicate asexually in the intestinal tissue before beginning sexual reproduction (58). On the other hand, when cats are orally infected with oocysts, the released sporozoites cannot start the sexual cycle in the gut of the cat; instead, they differentiate into the fast-dividing tachyzoite stage that subsequently converts into the bradyzoite stage, which only then can differentiate into intestinal stages (56, 59). This is demonstrated by the fact that the oocyst-shedding prepatent period greatly varies when cats get infected with cysts (usually less than one week) or oocysts (more than 2-3 weeks) (56, 58, 59). Because the hypothetical vaccine described above will no longer be able to form bradyzoites, it would be expected that cats orally infected with oocysts from such a strain will not shed oocysts, as intestinal stages cannot be formed in the absence of bradyzoites. Notwithstanding, this has never been categorically proven, and it cannot be ruled out that some epigenetic conditions in the intestine might trigger Toxoplasma differentiation into merozoites without having to go through the bradyzoite stage.
Regardless, it is possible that for the definitive hosts a bradyzoite-based vaccine would be more efficient. However, to obtain bradyzoites from a cyst-less strain, a conditional disruption of cyst-essential genes is needed. For example, by knocking out the BFD1 gene and replacing the endogenous locus with a regulatable BDF1 version, bradyzoite conversion could be tightly controlled (49). To achieve this goal, the expression of the BFD1 gene can be conditionally regulated by using the Tetracycline(Tet)-ON (60, 61) or the FKBP-derived destabilization domain (DD) system (49, 62). This way, by the addition of Tetracycline or the Shield-1 ligand, respectively, bradyzoites can be transiently formed and cysts obtained to feed cats orally. After infection, and without the presence of Tetracycline or Shield-1, the strain will not be able to form bradyzoites anymore. However, the formation of oocysts in the intestine after infection with such a strain cannot be avoided. To circumvent this shortcoming and prevent formation of infective oocysts, a gene essential for gamete formation, such as the aforementioned microgamete-specific HAP2 gene (43), could be disrupted. In addition, the bradyzoite-containing cysts needed for oral infection of cats could be obtained from the EGS strain, which has been shown to undergo high levels of spontaneous cyst conversion under regular conditions in vitro (63). Furthermore, these in vitro-derived EGS cysts were orally infective to cats, which produced oocysts in their feces (64). It could therefore be an interesting avenue to use this strain, or other highly cystogenic strain, as a parental parasite for the generation of the genetically modified vaccine described above, so that cats can be administered orally with in vitro cysts without the need to infect mice. In this sense, it could further help to use an in vitro system that generates fully functional orally infectious tissue cysts, such as the recently described human myotube-based in vitro culture model (65). Overall, if a cat can be vaccinated with cysts conditionally obtained in vitro from a strain that is not able to form sexual stages in the intestine nor convert into bradyzoites anymore, a protective immune response will be elicited in the cats while transmission is halted. A possible approach to design a hypothetical ideal vaccine based on oocysts is shown in Figure 1.
Figure 1 Layout of one of the possible approaches to devise a hypothetical oocyst-based ideal vaccine to prevent transmission in Toxoplasma. (1) A cystogenic strain, such as EGS, can be used as the parental strain to disrupt genes important for the dissemination of tachyzoites (TgWIP), in vivo fitness (MYR1), and bradyzoite conversion (BDF1). In addition, a construction in which an exogenous copy of BDF1 is conditionally regulated with the destabilizing domain (DD) can be inserted so that cyst formation can only be obtained in the presence of Shield-1. Finally, a plasmid containing a merozoite promoter (in this example the megakaryocyte stimulating factor -MSF-) driving the expression of the endonuclease Cas9 (epitope-tagged, in the example, with Flag) and several single guide (sg)RNAs cassettes targeting genes important for bradyzoite conversion and cyst formation, such as CST1, AP2XI-4 and BDF1, is also included. The latter is included as an additional safety measure in the unlikely scenario that the BDF1 exogenous copy introduced becomes active or leaky in the future. (2) Due to the presence of the merozoite promoter, the endonuclease Cas9 is not expressed in the tachyzoite stage. Moreover, since all the aforementioned targeted genes are dispensable in vitro, parasites can be grown normally in tissue culture. Finally, despite being a cystogenic strain, in the absence of Shield-1, the DD domain degrades BDF1 thus preventing tachyzoite-to-bradyzoite conversion (49). (3) Upon addition of Shield-1 to the culture media, BDF1 is constitutively expressed, strongly eliciting the conversion to the bradyzoite stage and cyst formation in vitro, while Cas9 is still not expressed. (4) Cysts obtained in vitro from the mutant EGS strain can be used to orally infect cats (64). (5) In the intestine of the cat, Cas9 is finally expressed and can be directed to knock out several genes involved in the tachyzoite-to-bradyzoite conversion, using specific guide RNA sequences present in the construction (see step 1). (6) Finally, the oocysts shed in the feces of the infected cat will be used to vaccinate other animals to elicit a protective immune response while preventing the parasite from converting to bradyzoites and forming tissue cysts. In the case of cats, this would also theoretically mean that intestinal stages will not be formed, as these can only be formed from bradyzoites, and thus oocysts will not be shed in the feces. U6: RNA polymerase III promoter; NLS: nuclear localization signal.
A Vaccine Based on Sporozoites
Despite being a critical stage for the transmission of Toxoplasma, sporozoites inside oocysts are under-studied, as they are not cultivatable in vitro and in vivo assays are restricted to a few laboratories with the resources to house cats. In contrast to tachyzoites, sporozoites within oocysts can stay viable outside a host for years even under harsh conditions. In addition, the oocyst and sporocyst walls provide an important protective barrier for sporozoites from environmental stressors. Therefore, a better understanding of the genes involved in extracellular survival and invasion of sporozoites present in oocysts could lead to the discovery of novel drug or vaccine targets that may prevent livestock or humans from getting infected by sporozoites. Furthermore, if the genetic basis for the extreme environmental resistance of sporozoites was known, it might be possible to exploit this to make other life stages, such as tachyzoites or other parasites, more viable extracellularly, which could enhance the shelf life of vaccines based on live parasites. Nonetheless, it is worth noting that this could lead to a potential misusage by creating Toxoplasma or other parasite mutant strains that are unnaturally resistant, constituting an ethical quandary (the recently coined “dual-use problem” term (66). However, this possibility exists for any Toxoplasma strain that is, for example, expressing a toxic heterologous protein, or using oocysts from any mutant strain. In this case, since the purpose is to use a recombinant strain deficient in dissemination with a deficient in vivo fitness, it would be unlikely that it can become more dangerous than a wild-type strain, even if tachyzoites were artificially made more viable extracellularly. Regardless, to avoid a morally undesirable usage, such hypothetical strains should be strictly contained and shared only under very specific and justified conditions. Moreover, it is also possible that transforming other life stages to make them more resistant by using genes related to environmental resistance could render them deficient in other aspects such as replication or undermine their natural behavior; for instance, it could be possible that tachyzoites would not be able to multiply at their usual fast pace anymore.
Be that as it may, we hypothesize that genes governing the resistance and infectivity of the sporozoite stage are specifically upregulated in sporulated oocysts compared to unsporulated oocysts. To identify such genes, we used data from a number of studies that examined the transcriptome and proteome of different Toxoplasma developmental stages, which are available on ToxoDB (67). We first identified 94 Toxoplasma proteins that had at least 10 unique peptides detected in at least one of the two proteomic analyses of oocysts but no peptides in any other stages (68, 69). We subsequently refined this list by identifying 34 Toxoplasma genes that are on average at least 4-fold upregulated in sporulated oocysts (day 4 and 10 after shedding) vs. unsporulated oocysts (day 0 after shedding) and vs. tachyzoites, bradyzoites and merozoites (43, 70, 71). We noted that among the highest expressed genes (Table 1) there are 4 genes that encode for putative late embryogenesis abundant proteins (LEAs). LEA proteins have been described in several organisms, including plants, invertebrates, and microorganisms, with a commonly ascribed role in resistance to environmental stresses such as drought, high salinity and freezing (72). Their abundance in sporozoites suggests that LEA proteins may be a critical component to sporozoites. One of the LEA proteins (TGME49_276850 also named TgERP -Embryogenesis-Related Protein-) was used in a serological assay and shown to be able to distinguish infections caused by ingestion of oocysts vs. tissue cysts (73). Some other interesting genes from our search included a DNA photolyase (TGME49_206400) that could be involved in DNA-repair mechanisms caused by UV light (74, 75) and a putative glutaredoxin-like protein (TGME49_227100) that could be related to oxidation repair enzyme processes (76, 77).
Table 1 Highly upregulated sporozoite genes candidate list.
Moreover, multiple genes in Table 1 are predicted to be involved in sporozoite attachment/invasion. For example, Sporo-Surface Antigen (SAG), also called SAG1-related Sequence (SRS)28, is predicted to be involved in parasite attachment to negatively charged host surface molecules such as glycosaminoglycans (78), and tachyzoites overexpressing SporoSAG have enhanced invasion in HFFs (79). SporoAMA1 and SporoRON2 have been shown to be involved in parasite invasion, as the addition of recombinant sporoRON2-domain 3, which competes with the SporoAMA1-sporoRON2 interaction, significantly inhibited sporozoite invasion (79). However, Toxoplasma has 4 AMA-1 like genes and 3 RON2-like genes, combinations of which are differentially expressed in different life stages. Therefore, other AMA-RON pairs can compensate for the absence of specific pairs (80) and, besides sporoSAG, it is predicted that sporozoites express at least another 10 SRS proteins. Hence, it is unlikely that only one is sufficient to prevent sporozoite invasion, which would mean that immunization with a SRS, sporoAMA or sporoRON2 based vaccine might not be successful. TGME49_259670 has significant homology to the Plasmodium circumsporozoite protein- and thrombospondin-related anonymous protein (TRAP)-related protein (CTRP). CTRP is expressed in the Plasmodium ookinete, and disruption of this gene leads to reduced motility and failure to invade the mosquito midgut epithelium (81). Furthermore, CTRP is part of a protein family, which includes TgMIC2 and Plasmodium TRAP, that is involved in motility and invasion (82). TgME49_209920 is a gene encoding for a putative PAN domain-containing protein with homology to microneme protein (MIC)-4 (MIC4-Like). Toxoplasma MIC genes have been shown to play a role in attachment/invasion (83). It is therefore possible that TGME49_259670 and MIC4-Like are involved in the motility and invasion mechanisms of sporozoites.
In summary, genes involved in sporozoite invasion or motility might be good targets for the development of new drugs and vaccines against toxoplasmosis. In addition, the overexpression of genes important for the resistance of sporozoites, such as LEAs, could make the live-attenuated vaccine strains stable and facilitate their production in vitro.
Conclusions and Future Perspectives
Cats serve an important epidemiological role in spreading toxoplasmosis, as they are the only host able to shed oocysts in their feces. Although live-attenuated vaccines can partially protect immunized animals against subsequent infections, full protection is rarely attained. Moreover, vaccines based on mutant strains that are deficient in forming the chronic phase of the parasite cannot be extensively used due to the possibility of reversion to virulent phenotypes. In order to design novel live-attenuated vaccines with known genetic modifications that can be easily stored in the long term, we propose a strategy based on environmentally resistant chronic forms of Toxoplasma (oocysts and tissue cysts). For example, by targeting genes that are important for the in vivo fitness of the parasite, conversion to the bradyzoite stage and cyst formation, an “oocyst vaccine” could be obtained that elicits a protective immune response while preventing transmission. Although there is a challenge to produce oocysts from such a strain, as the parasite first needs to complete the whole life cycle in cats, recent cutting-edge engineering techniques make it possible to disrupt targeted genes only after reaching the cat’s intestine, when they are no longer needed. Moreover, further improvements and refinement of the groundbreaking systems described by Di Genova et al. (53), Farhat et al. (54) and Humayun et al. (55) might lead to the in vitro production of oocysts from such a mutant strain without the need for using mice or cats. Finally, a better understanding of the genes involved in invasion and resistance of sporozoites present in the oocysts could lead to the discovery of valuable drug or vaccine targets that may prevent animals from getting infected and therefore spread the disease to other animals or humans.
Because oocysts are highly resistant and infective, a single-time generation of oocyst would suffice to potentially vaccinate thousands of animals in a short period of time without the need for special storage, significantly facilitating the task. Notwithstanding, it is worth noting that the implementation of this vaccination strategy would face many hurdles along the way. For instance, it would be theoretically possible that a recombination event might take place in the intestine of potentially vaccinated cats harboring other wild-type strains and thus generate parasites with reversion to, or mixture of, wild-type features. This scenario would be highly unlikely to occur in domestic cats with limited, or lack of, outdoor activities, as intestinal stages are only formed in the initial stages after infection and thus vaccination with the recombinant strain would need to happen at a very specific and short span of time, provided they were even previously infected. However, in a more natural setup, for example in outdoor domestic cats or wild felids, it would not be unfeasible to have one or many concurrent infections with other natural strains at the time of vaccination that might elicit this potential undesired situation. Nevertheless, this issue could be easily addressed by first ensuring the negative immunologic status of the animals to be vaccinated (i.e. by serology) before doing so.
Another hindrance would be to perform the vaccination in as many cats as possible, including both domestic and wild felines, within a geographical location. For the former, apart from offering the immunization free of charge, the willingness of each owner needs to be considered. Hence, measurements including educational information and discussion of potential benefits, as well as incentives (for example free serological and coprological analysis, or complimentary regular vaccination/deparasitation treatments) should be implemented. As for wild felids, joint efforts would be required to capture animals, assess their status as mentioned above and perform oral inoculation under sedation before releasing them back into their habitat. Moreover, it is unrealistic to reach all animals, either domestic or wild, outside secluded venues; nevertheless, even vaccination of some animals in a specific location could lead to a significant reduction in the exposure to oocysts of other animals and humans (32), having a significant positive impact in both animal and public health.
Data Availability Statement
The original contributions presented in the study are included in the article/supplementary material. Further inquiries can be directed to the corresponding author.
Author Contributions
DA-S and JS have contributed to the conceptualization, writing, and editing of this manuscript. Both authors contributed to the article and approved the submitted version.
Funding
This study was supported by the National Institutes of Health (1R21AI139387-01) awarded to JS. DA-S was partially supported by the Center for Companion Animal Health (CCAH) at UC Davis with the following grants: 2016-21-F, 2017-11-F, 2018-53-F and 2019-12-F.
Conflict of Interest
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Publisher’s Note
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Keywords: Toxoplasma, CRISPR/Cas9, oocysts, cysts, sporozoites, vaccine, cats
Citation: Arranz-Solís D and Saeij JPJ (2022) New Avenues to Design Toxoplasma Vaccines Based on Oocysts and Cysts. Front. Immunol. 13:910961. doi: 10.3389/fimmu.2022.910961
Received: 01 April 2022; Accepted: 17 May 2022;
Published: 06 June 2022.
Edited by:
Delphine Le Roux, UMR BIPAR (Anses, EnvA, INRAE) École Nationale Vétérinaire d’Alfort, FranceReviewed by:
Tiago W. P. Mineo, Federal University of Uberlandia, BrazilCopyright © 2022 Arranz-Solís and Saeij. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Jeroen P. J. Saeij, jsaeij@ucdavis.edu