AUTHOR=van der Sijde Fleur , Dik Willem A. , Mustafa Dana A. M. , Vietsch Eveline E. , Besselink Marc G. , Debets Reno , Koerkamp Bas Groot , Haberkorn Brigitte C. M. , Homs Marjolein Y. V. , Janssen Quisette P. , Luelmo Saskia A. C. , Mekenkamp Leonie J. M. , Oostvogels Astrid A. M. , Smits-te Nijenhuis Marja A. W. , Wilmink Johanna W. , van Eijck Casper H. J. ,  the Dutch Pancreatic Cancer Group 

TITLE=Serum cytokine levels are associated with tumor progression during FOLFIRINOX chemotherapy and overall survival in pancreatic cancer patients

JOURNAL=Frontiers in Immunology

VOLUME=13

YEAR=2022

URL=https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2022.898498

DOI=10.3389/fimmu.2022.898498

ISSN=1664-3224

ABSTRACT=<sec><title>Background</title><p>Biomarkers predicting treatment response may be used to stratify patients with pancreatic ductal adenocarcinoma (PDAC) for available therapies. The aim of this study was to evaluate the association of circulating cytokines with FOLFIRINOX response and with overall survival (OS).</p></sec><sec><title>Methods</title><p>Serum samples were collected before start and after the first cycle of FOLFIRINOX from patients with PDAC (<italic>n</italic>=83) of all disease stages. Overall, 34 circulating cytokines were analyzed with a multiplex immunoassay. In addition, changes in peripheral blood immune cell counts were determined by flow cytometry to correlate with differences in cytokine levels. Chemotherapy response was determined by CT scans with the RECIST 1.1 criteria, as disease control (<italic>n</italic>=64) or progressive disease (<italic>n</italic>=19) within eight cycles of FOLFIRINOX.</p></sec><sec><title>Results</title><p>Patients with high serum IL-1RA concentrations after one cycle of chemotherapy were less likely to have tumor progression during FOLFIRINOX (OR 0.25, <italic>P</italic>=0.040). Increase of circulating IL-1RA concentrations correlated with increase of total, classical (CD14+CD16-), and non-classical monocytes (CD14-CD16+), and dendritic cells. In multivariable cox regression, including the variables chemotherapy response outcome and baseline CA19-9 level, serum concentrations of IL-7 (HR 2.14, <italic>P</italic>=0.010), IL-18 (HR 2.00, <italic>P</italic>=0.020), and MIP-1β (HR 0.51, <italic>P</italic>=0.025) after one cycle of FOLFIRINOX showed correlations with OS.</p></sec><sec><title>Conclusions</title><p>Circulating IL-1RA, IL-7, IL-18, and MIP-1β concentrations are biomarkers associated with FOLFIRINOX response in PDAC patients, suggesting an important role for specific immune cells in chemotherapy response and PDAC progression. Cytokine-based treatment might improve patient outcome and should be evaluated in future studies.</p></sec>