AUTHOR=Yin Zhihui , Nie Hongtao , Jiang Kunyin , Yan Xiwu 

TITLE=Molecular Mechanisms Underlying Vibrio Tolerance in Ruditapes philippinarum Revealed by Comparative Transcriptome Profiling

JOURNAL=Frontiers in Immunology

VOLUME=13

YEAR=2022

URL=https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2022.879337

DOI=10.3389/fimmu.2022.879337

ISSN=1664-3224

ABSTRACT=<p>The clam <italic>Ruditapes philippinarum</italic> is an important species in the marine aquaculture industry in China. However, in recent years, the aquaculture of <italic>R. philippinarum</italic> has been negatively impacted by various bacterial pathogens. In this study, the transcriptome libraries of <italic>R. philippinarum</italic> showing different levels of resistance to challenge with <italic>Vibrio anguillarum</italic> were constructed and RNA-seq was performed using the Illumina sequencing platform. Host immune factors were identified that responded to <italic>V. anguillarum</italic> infection, including C-type lectin domain, glutathione S-transferase 9, lysozyme, methyltransferase FkbM domain, heat shock 70 kDa protein, Ras-like GTP-binding protein RHO, C1q, F-box and BTB/POZ domain protein zf-C2H2. Ten genes were selected and verified by RT-qPCR, and nine of the gene expression results were consistent with those of RNA-seq. The lectin gene in the phagosome pathway was expressed at a significantly higher level after <italic>V. anguillarum</italic> infection, which might indicate the role of lectin in the immune response to <italic>V. anguillarum</italic>. Comparing the results from <italic>R. philippinarum</italic> resistant and nonresistant to <italic>V. anguillarum</italic> increases our understanding of the resistant genes and key pathways related to <italic>Vibrio</italic> challenge in this species. The results obtained here provide a reference for future immunological research focusing on the response of <italic>R. philippinarum</italic> to <italic>V. anguillarum</italic> infection.</p>