AUTHOR=Pande Kalyan , Hollingsworth Scott A. , Sam Miranda , Gao Qinshan , Singh Sujata , Saha Anasuya , Vroom Karin , Ma Xiaohong Shirley , Brazell Tres , Gorman Dan , Chen Shi-Juan , Raoufi Fahimeh , Bailly Marc , Grandy David , Sathiyamoorthy Karthik , Zhang Lan , Thompson Rob , Cheng Alan C. , Fayadat-Dilman Laurence , Geierstanger Bernhard H. , Kingsley Laura J. 

TITLE=Hexamerization of Anti-SARS CoV IgG1 Antibodies Improves Neutralization Capacity

JOURNAL=Frontiers in Immunology

VOLUME=13

YEAR=2022

URL=https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2022.864775

DOI=10.3389/fimmu.2022.864775

ISSN=1664-3224

ABSTRACT=<p>The SARS-CoV-2 pandemic and particularly the emerging variants have deepened the need for widely available therapeutic options. We have demonstrated that hexamer-enhancing mutations in the Fc region of anti-SARS-CoV IgG antibodies lead to a noticeable improvement in IC<sub>50</sub> in both pseudo and live virus neutralization assay compared to parental molecules. We also show that hexamer-enhancing mutants improve C1q binding to target surface. To our knowledge, this is the first time this format has been explored for application in viral neutralization and the studies provide proof-of-concept for the use of hexamer-enhanced IgG1 molecules as potential anti-viral therapeutics.</p>