AUTHOR=Wang Xue , Qu Yunhe , Wang Yuan , Wang Xiang , Xu Jialei , Zhao Hailing , Zheng Donglin , Sun Lin , Tai Guihua , Zhou Yifa , Cheng Hairong 

TITLE=β-1,6-Glucan From Pleurotus eryngii Modulates the Immunity and Gut Microbiota

JOURNAL=Frontiers in Immunology

VOLUME=13

YEAR=2022

URL=https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2022.859923

DOI=10.3389/fimmu.2022.859923

ISSN=1664-3224

ABSTRACT=<p>Polysaccharides from <italic>Pleurotus eryngii</italic> exhibit a variety of biological activities. Here, we obtained a homogeneous branched β-1,6-glucan (APEP-A-b) from the fruiting bodies of <italic>P. eryngii</italic> and investigated its effect on immunity and gut microbiota. Our results showed that APEP-A-b significantly increases splenic lymphocyte proliferation, NK cell activity and phagocytic capacity of peritoneal cavity phagocytes. Furthermore, we found that the proportion of CD4<sup>+</sup> and CD8<sup>+</sup> T cells in lamina propria are significantly increased upon APEP-A-b treatment. Additionally, APEP-A-b supplementation demonstrated pronounced changes in microbiota reflected in promotion of relative abundances of species in the Lachnospiraceae and Rikenellaceae families. Consistently, APEP-A-b significantly increased the concentration of acetic and butyric acid in cecum contents. Overall, our results suggest that β-1,6-glucan from P. eryngii might enhance immunity by modulating microbiota. These results are important for the processing and product development of P. eryngii derived polysaccharides.</p>