AUTHOR=Lioulios Georgios , Fylaktou Asimina , Xochelli Aliki , Sampani Erasmia , Tsouchnikas Ioannis , Giamalis Panagiotis , Daikidou Dimitra-Vasilia , Nikolaidou Vasiliki , Papagianni Aikaterini , Theodorou Ioannis , Stangou Maria TITLE=Clustering of End Stage Renal Disease Patients by Dimensionality Reduction Algorithms According to Lymphocyte Senescence Markers JOURNAL=Frontiers in Immunology VOLUME=13 YEAR=2022 URL=https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2022.841031 DOI=10.3389/fimmu.2022.841031 ISSN=1664-3224 ABSTRACT=
End stage renal disease (ESRD) engenders detrimental effects in the Immune system, manifested as quantitative alterations of lymphocyte subpopulations, akin, albeit not identical to those observed during the ageing process. We performed dimensionality reduction of an extended lymphocyte phenotype panel of senescent and exhaustion related markers in ESRD patients and controls with Principal Component Analysis (PCA) and Uniform Manifold Approximation and Projection (UMAP). The plane defined by the first two principal components of PCA showed two fuzzy clusters, for patients and controls, respectively, with loadings of non-senescent markers pointing towards the controls’ centroid. Naive lymphocytes were reduced in ESRD patients compared to controls (CD4+CD45RA+CCR7+ 200(150-328)