AUTHOR=Yu Dianjiang , Zhai Yufeng , He Peimin , Jia Rui 

TITLE=Comprehensive Transcriptomic and Metabolomic Analysis of the Litopenaeus vannamei Hepatopancreas After WSSV Challenge

JOURNAL=Frontiers in Immunology

VOLUME=13

YEAR=2022

URL=https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2022.826794

DOI=10.3389/fimmu.2022.826794

ISSN=1664-3224

ABSTRACT=<p><italic>Litopenaeus vannamei</italic> is the major farmed shrimp species worldwide. White spot disease due to white spot syndrome virus (WSSV) is severely affecting shrimp worldwide, causing extensive economic losses in <italic>L. vannamei</italic> culture. This is the first study that applied combined transcriptomic and metabolomic analysis to study the effects on the <italic>L. vannamei</italic> hepatopancreas after WSSV challenge. Our transcriptomic data revealed differentially expressed genes (DEGs) associated with immunity, apoptosis, the cytoskeleton and the antioxidant system in the hepatopancreas of <italic>L. vannamei</italic>. Metabolomic results showed that WSSV disrupts metabolic processes including amino acid metabolism, lipid metabolism and nucleotide metabolism. After challenged by WSSV, immune-related DEGs and differential metabolites (DMs) were detected in the hepatopancreas of <italic>L. vannamei</italic>, indicating that WSSV may damage the immune system and cause metabolic disorder in the shrimp. In summary, these results provide new insights into the molecular mechanisms underlying <italic>L. vannamei</italic>’s response to WSSV.</p>