AUTHOR=Li Qiao , Ye Chenglin , Zhao Fei , Li Wenjin , Zhu Sizhe , Lv Yin , Park Chae Gyu , Zhang Yingmiao , Jiang Ling-Yu , Yang Kun , He Yingxia , Cai Huahua , Zhang Song , Ding Hong-Hui , Njiri Olivia Adhiambo , Tembo John Mambwe , Alkraiem Ayman Ahmad , Li An-Yi , Sun Zi-Yong , Li Wei , Yan Mei-Ying , Kan Biao , Huo Xixiang , Klena John D. , Skurnik Mikael , Anisimov Andrey P. , Gao Xiaofang , Han Yanping , Yang Rui-Fu , Xiamu Xiding , Wang Yuanzhi , Chen Hongxiang , Chai Bao , Sun Yicheng , Yuan Jingping , Chen Tie 

TITLE=PgtE Enzyme of Salmonella enterica Shares the Similar Biological Roles to Plasminogen Activator (Pla) in Interacting With DEC-205 (CD205), and Enhancing Host Dissemination and Infectivity by Yersinia pestis

JOURNAL=Frontiers in Immunology

VOLUME=13

YEAR=2022

URL=https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2022.791799

DOI=10.3389/fimmu.2022.791799

ISSN=1664-3224

ABSTRACT=<p><italic>Yersinia pestis</italic>, the cause of plague, is a newly evolved Gram-negative bacterium. Through the acquisition of the plasminogen activator (Pla), <italic>Y. pestis</italic> gained the means to rapidly disseminate throughout its mammalian hosts. It was suggested that <italic>Y. pestis</italic> utilizes Pla to interact with the DEC-205 (CD205) receptor on antigen-presenting cells (APCs) to initiate host dissemination and infection. However, the evolutionary origin of Pla has not been fully elucidated. The PgtE enzyme of <italic>Salmonella enterica</italic>, involved in host dissemination, shows sequence similarity with the <italic>Y. pestis</italic> Pla. In this study, we demonstrated that both <italic>Escherichia coli</italic> K-12 and <italic>Y. pestis</italic> bacteria expressing the PgtE-protein were able to interact with primary alveolar macrophages and DEC-205-transfected CHO cells. The interaction between PgtE-expressing bacteria and DEC-205-expressing transfectants could be inhibited by the application of an anti-DEC-205 antibody. Moreover, PgtE-expressing <italic>Y. pestis</italic> partially re-gained the ability to promote host dissemination and infection. In conclusion, the DEC-205-PgtE interaction plays a role in promoting the dissemination and infection of <italic>Y. pestis</italic>, suggesting that Pla and the PgtE of <italic>S. enterica</italic> might share a common evolutionary origin.</p>