AUTHOR=Barrows Brittani M. , Krebs Shelly J. , Jian Ningbo , Zemil Michelle , Slike Bonnie M. , Dussupt Vincent , Tran Ursula , Mendez-Rivera Letzibeth , Chang David , O’Sullivan Anne Marie , Mann Brendan , Sanders-Buell Eric , Shubin Zhanna , Creegan Matt , Paquin-Proulx Dominic , Ehrenberg Philip , Laurence-Chenine Agnes , Srithanaviboonchai Kriengkrai , Thomas Rasmi , Eller Michael A. , Ferrari Guido , Robb Merlin , Rao Venigalla , Tovanabutra Sodsai , Polonis Victoria R. , Wieczorek Lindsay TITLE=Fc receptor engagement of HIV-1 Env-specific antibodies in mothers and infants predicts reduced vertical transmission JOURNAL=Frontiers in Immunology VOLUME=13 YEAR=2022 URL=https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2022.1051501 DOI=10.3389/fimmu.2022.1051501 ISSN=1664-3224 ABSTRACT=Introduction

Infants acquire maternal antibodies by Fc receptor transcytosis across the placenta during pregnancy. Fc receptors are expressed on immune cells and are important for activation of effector cell functions.

Methods

In this study, we evaluated Fc receptor engagement and ADCC activity of plasma binding antibodies from human immunodeficiency virus-1 (HIV) -infected mothers and to identify factors that may contribute to protection from HIV vertical transmission.

Results

HIV-specific binding and Fc receptor engagement of plasma antibodies varied between mothers by transmission status and infants by infection status. Non-transmitting (NT) mothers and HIV-uninfected infants had antibodies with higher neonatal Fc receptor (FcRn) and FcγR engagement, as compared to transmitting (T) mothers and HIV+ infants, respectively. A significant inverse correlation between plasma antibody FcRn and FcγR engagement was observed for T mothers, but not NT mothers. Conversely, a significant direct correlation was observed between plasma antibody FcRn and FcγR engagement for HIV- infants, but not for HIV+ infants. Consequently, we observed significantly higher plasma antibody ADCC potency and breadth in HIV- infants, as compared to HIV+ infants. However, no differences in overall ADCC potency and breadth were observed between mothers. FcRn-engagement of HIV-specific antibodies in both mothers and infants predicted a lack of vertical transmission of HIV.

Discussion

This study indicates that HIV-uninfected infants acquire HIV-specific antibodies with greater Fc receptor engagement and thus, greater ADCC capacity.