AUTHOR=Zoodsma Martijn , de Nooijer Aline H. , Grondman Inge , Gupta Manoj Kumar , Bonifacius Agnes , Koeken Valerie A. C. M. , Kooistra Emma , Kilic Gizem , Bulut Ozlem , Gödecke Nina , Janssen Nico , Kox Matthijs , Domínguez-Andrés Jorge , van Gammeren Adriaan J. , Ermens Anton A. M. , van der Ven Andre J. A. M. , Pickkers Peter , Blasczyk Rainer , Behrens Georg M. N. , van de Veerdonk Frank L. , Joosten Leo A. B. , Xu Cheng-Jian , Eiz-Vesper Britta , Netea Mihai G. , Li Yang TITLE=Targeted proteomics identifies circulating biomarkers associated with active COVID-19 and post-COVID-19 JOURNAL=Frontiers in Immunology VOLUME=13 YEAR=2022 URL=https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2022.1027122 DOI=10.3389/fimmu.2022.1027122 ISSN=1664-3224 ABSTRACT=

The ongoing Coronavirus Disease 2019 (COVID-19) pandemic is caused by the highly infectious Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2). There is an urgent need for biomarkers that will help in better stratification of patients and contribute to personalized treatments. We performed targeted proteomics using the Olink platform and systematically investigated protein concentrations in 350 hospitalized COVID-19 patients, 186 post-COVID-19 individuals, and 61 healthy individuals from 3 independent cohorts. Results revealed a signature of acute SARS-CoV-2 infection, which is represented by inflammatory biomarkers, chemokines and complement-related factors. Furthermore, the circulating proteome is still significantly affected in post-COVID-19 samples several weeks after infection. Post-COVID-19 individuals are characterized by upregulation of mediators of the tumor necrosis (TNF)-α signaling pathways and proteins related to transforming growth factor (TGF)-ß. In addition, the circulating proteome is able to differentiate between patients with different COVID-19 disease severities, and is associated with the time after infection. These results provide important insights into changes induced by SARS-CoV-2 infection at the proteomic level by integrating several cohorts to obtain a large disease spectrum, including variation in disease severity and time after infection. These findings could guide the development of host-directed therapy in COVID-19.