Skip to main content

ORIGINAL RESEARCH article

Front. Immunol., 08 December 2022
Sec. Parasite Immunology
This article is part of the Research Topic Sensing, directing, effecting: innate immune cell function in parasitic infections View all 4 articles

Trickle infection with Heligmosomoides polygyrus results in decreased worm burdens but increased intestinal inflammation and scarring

Updated
Anupama Ariyaratne,Anupama Ariyaratne1,2Sang Yong KimSang Yong Kim3Stephen M. J. Pollo,Stephen M. J. Pollo2,4Shashini Perera,Shashini Perera1,2Hongrui Liu,Hongrui Liu1,2William N. T. NguyenWilliam N. T. Nguyen5Aralia Leon Coria,Aralia Leon Coria1,2Mayara de Cassia Luzzi,Mayara de Cassia Luzzi1,2Joel Bowron,Joel Bowron1,2Edina K. Szabo,Edina K. Szabo1,2Kamala D. PatelKamala D. Patel5James D. Wasmuth,James D. Wasmuth2,4Meera G. NairMeera G. Nair3Constance A. M. Finney,*Constance A. M. Finney1,2*
  • 1Department of Biological Sciences, Faculty of Science, University of Calgary, Calgary, AB, Canada
  • 2Host Parasite Interactions Training Network, University of Calgary, Calgary, AB, Canada
  • 3Division of Biomedical Sciences, School of Medicine, University of California Riverside, Riverside, CA, United States
  • 4Faculty of Veterinary Medicine, University of Calgary, Calgary, AB, Canada
  • 5Departments of Physiology and Pharmacology, Faculty of Medicine, University of Calgary, Calgary, AB, Canada

Introduction: Intestinal roundworms cause chronic debilitating disease in animals, including humans. Traditional experimental models of these types of infection use a large single-dose infection. However, in natural settings, hosts are exposed to parasites on a regular basis and when mice are exposed to frequent, smaller doses of Heligmosomoides polygyrus, the parasites are cleared more quickly. Whether this more effective host response has any negative consequences for the host is not known.

Results: Using a trickle model of infection, we found that worm clearance was associated with known resistance-related host responses: increased granuloma and tuft cell numbers, increased levels of granuloma IgG and decreased intestinal transit time, as well as higher serum IgE levels. However, we found that the improved worm clearance was also associated with an inflammatory phenotype in and around the granuloma, increased smooth muscle hypertrophy/hyperplasia, and elevated levels of Adamts gene expression.

Discussion: To our knowledge, we are the first to identify the involvement of this protein family of matrix metalloproteinases (MMPs) in host responses to helminth infections. Our results highlight the delicate balance between parasite clearance and host tissue damage, which both contribute to host pathology. When continually exposed to parasitic worms, improved clearance comes at a cost.

Introduction

Gastrointestinal parasitic nematodes often cause chronic recurring infections. Hosts mount a strong immune response to nematode parasites, essential to control worm burden as well as host tissue damage. While many hosts are infected and unable to clear their infection, they can limit excessively damaging worm burdens, implying immune regulatory mechanisms are at play (1). The balance and efficacy of the response is dependent on parasite killing and wound healing mechanisms that act in concert to maximize host fitness.

Heligmosomoides polygyrus is an enteric nematode parasite of mice (2, 3). Ingested larvae encyst in the host intestinal wall and mature into adults that escape into the lumen. This process takes approximately one week, after which adults remain in the intestinal lumen for the duration of infection. H. polygyrus tissue-dwelling stages cause the release of alarmins from epithelial cells (including tuft cells) as they damage the intestinal wall (1, 4, 5). Alarmin-activated innate lymphoid cells and Th2 polarized CD4+ T cells produce Th2 cytokines (69) which promote innate immune cell influx to the intestine (3, 6, 10). The accumulation of immune cells is referred to as a granuloma (7, 11, 12) and an increase in granuloma size and number is associated with increased resistance to H. polygyrus (13).

Granulomas can be categorized as early-stage, containing developing worms, and late-stage, where worms have escaped into the intestinal lumen, and/or been killed. Within the early-stage granuloma, the immune response must balance the dual demands of incapacitating or killing the developing nematode, while also healing the damage caused by the growing worm. In late-stage granulomas, the immune response has shifted to focus on healing [reviewed in (14)]. Eliminating the tissue stage parasites relies on antibody-dependent cell-mediated cytotoxicity (ADCC) by macrophages and eosinophils (7, 15, 16), the main cellular players within the granuloma. AAMs and eosinophils produce immunoregulatory and wound healing molecules (17, 18) such as Ym1, RELM-α (3, 19, 20), and Arginase 1 (9, 21), which promote extracellular matrix (ECM) deposition during helminth infections (2225). This healing process has been associated with fibrosis and scarring during chronic helminth infection through the excessive deposition of collagen, a major component of the ECM (26). How the host balances parasite clearance and effective tissue remodeling within the granuloma is not fully understood.

Immune responses are not only generated to tissue dwelling parasitic stages but also to the adult worms found in the intestinal lumen. The cytokines IL-4 and IL-13 enhance smooth muscle contractility of the intestine via STAT6 dependent pathways (27) to help eliminate adult worms (2830). IL-4, IL-9, and IL-13 regulate goblet cell hyperplasia and increase mucus production during gastrointestinal (GI) nematode infections (31, 32) which presumably makes it more difficult for adult parasites to coil around intestinal villi. H. polygyrus infections also induce polyclonal and parasite-specific antibody responses, which function to damage worm larvae as they develop and limit adult female egg production (1, 3335).

Most of the murine studies on helminth infection use a bolus model of infection (one large dose), with some groups adopting a drug clearance model (bolus infection, drug clearance, bolus infection) to simulate mass drug administration programs in human populations (7, 33). However, under natural conditions, gastrointestinal nematodes are ubiquitous in the environment (36) and hosts are constantly encountering them. Hence, we and others have set up experimental infection models using trickle infections to study host-nematode infections in a more natural setting (3741). We use multiple low doses of larvae, given over a specific time period to achieve this.

H. polygyrus trickle infections in genetically resistant and susceptible strains of mice reveal that the frequency of infection is an important determinant of parasite expulsion, where frequently infected mice eliminate worms more rapidly than mice infected with the same total number of larvae but in less frequent doses (40). Others have implicated improved antibody and innate immune cell responses to tissue dwelling parasites as key elements for the reduced worm burdens observed during trickle infections (37, 40, 41). In our model, improved clearance was associated with known resistance-related host responses: increased granuloma and tuft cell numbers, increased levels of granuloma IgG, decreased intestinal transit time and higher serum IgE levels. However, we found that the improved worm clearance observed in trickle-infected animals was also associated with an inflammatory phenotype in and around the granuloma, increased smooth muscle hypertrophy/hyperplasia and elevated Adamts gene expression. Many studies have focused on understanding the mechanisms involved in damaging/killing worms, but fewer have focused on the wound healing processes involved in creating and resorbing granulomas. This is key, as there is a delicate balance between parasite clearance and host pathology which ultimately impact host fitness.

Materials and methods

Mice, parasites, and antigen

Female and male C57Bl/6 and BALB/c mice aged 6-8 weeks (bred and maintained at the animal care facility, Department of Biological Sciences, University of Calgary, Canada or University of California, Riverside, USA) were used. All animal experiments were approved by the University of Calgary’s Life and Environmental Sciences Animal Care Committee (protocol AC17-0083) and the University of California, Riverside’s Institutional Animal Care and Use Committee (https://or.ucr.edu/ori/committees/iacuc.aspx; protocol A-20180023). All protocols for animal use and euthanasia were in accordance with either the Canadian Council for Animal Care (Canada) or National Institutes of Health (USA) guidelines. Animal studies are in accordance with the provisions established by the Animal Welfare Act and the Public Health Services (PHS) Policy on the Humane Care and Use of Laboratory Animals.

Infected mice were orally gavaged with 200 third stage Heligmosomoides polygyrus larvae (maintained in house, stock was a gift from Dr. Allen Shostak, University of Alberta, Canada and Dr Lisa Reynolds,University of Victoria, Canada) and euthanized at either 7 (D7), 14 (D14) or 21 (D21) days post initial infection. Mice were infected according to the bolus or trickle infection regimes (Figure 1A and Supplementary Figure 1A). To avoid differences in counts during the trickle infections, on day 0, two identical solutions were made up (200 worms/100ul). One was used to infect the bolus infected mice on day 0 and one was used for the trickle-infected mice. The solution for the trickle-infected animals was divided into six equal parts. Each part was made up to 100ul using water. Animals were gavaged with a diluted solution on days 0, 2, 4, 6, 8, 10. Using the 6 doses, trickle-infected animals received 200 larvae in total over 10 days.

Figure 1
www.frontiersin.org

Figure 1 The reduced worm burden in trickle-infected C57Bl/6 mice is associated with elevated serum IgE. 6-8 week old C57Bl/6 and BALB/c mice were infected with 200 H polygyrus larvae according to the bolus and trickle infection regimes. (A) Trickle infection regime: mice are infected with 200 larvae in total, but in multiple doses over the course of infection (in grey). Doses of ~33 larvae are trickled on days 0, 2, 4, 6, 8 and 10 post-infection. There is a 10-day window after the final dose to allow parasites to fully develop into adults and migrate from the intestinal tissue to the intestinal lumen. (B) Adult worms were counted in the small intestine using a dissection microscope. (C) Single cell suspensions were isolated from the mesenteric lymph nodes and spleens. Viable cell numbers in the MLN (left) and SPL (right). Single cell suspensions were either used for flow cytometry or cultured for 48 hours in the presence of H polygyrus antigen. (D) Percentage of Foxp3+ cells within the CD4+ population of MLN cells were measured by flow cytometry. (E) IL-4 (MLN and SPL) and (F) IL-13 (MLN and SPL) cytokine levels were measured in the supernatant by ELISA. Serum antibody levels were measured by ELISA for (G) parasite specific IgG1 and (H) total IgE. Levels in naïve controls were undetectable for parasite specific IgG1. For all panels except D, graphs represent pooled data from 2 experiments, bars represent the median, with a minimum of 3 mice per group per experiment. For panel D, each circle represents one experiment using cells pooled from 5 mice. BALB/c mice (white mouse) and C57Bl/6 mice (black mouse) were infected according to the bolus (black circles) and trickle (white circles) regimes. A normality test was performed (Anderson-Darling) followed by a Kruskal Wallis test with Dunn’s multiple comparisons test to test for statistical significance between trickle and bolus groups; for panel (D), a paired T-test was performed; n.s., not significant; *p<0.05, ****p<0.0001.

H. polygyrus antigen was prepared by collecting live adult worms from 14-day infected mice using modified Baerman’s apparatus. Worms were washed multiple times and homogenized in PBS using a glass homogenizer. The resulting solution was centrifuged (13, 000 g, 10 minutes, 4°C) and the supernatant filtered (0.2μm filter, Nalgene). The protein concentration was calculated using the Bradford assay. The antigen was stored at 15 mg/ml at -80°C.

Adult worm burden and granuloma number

Small intestines of infected mice were harvested and opened longitudinally. The number of adult worms present in the intestinal lumen and of granulomas present along the length of the small intestine were counted using a dissection microscope.

Transit time

Gastrointestinal transit time was measured one day prior to euthanasia. Mice were fasted for 6 hours and 200 μl of 5% Evans blue (Sigma) in 5% gum arabic (ACROS organics) was orally gavaged using a ball tip 20 gauge 1.5’’, 2.25mm curved animal feeding needle. Each mouse was labelled, with the time of dye administration recorded. Mice were transferred to clean empty cages and the time to pass the first blue fecal pellet was recorded. Gastrointestinal transit time was calculated for each mouse.

Cell isolation and in vitro re-stimulation assay

Mesenteric lymph nodes (MLN) and spleens (SPL) were mechanically dissociated into single cell suspensions. Cells were counted using a Beckman-Coulter ViCell XR. MLN and SPL were cultured at 1 x 106 cells/ml for 48 hours in RPMI medium, 10% FCS, 1% L-glutamine, 1% penicillin/streptomycin (supplemented RPMI 1640) in the presence of 10 μg/ml H. polygyrus antigen or 2 μg/ml concanavalin A (Sigma) at 370C with 5% CO2. Supernatants were collected for cytokine measurements. Measurements for antigen-specific production were not included in the analysis unless cytokine production was observed in the wells with concanavalin A stimulation.

Flow cytometry

MLN single cell suspensions were stained and analyzed according to (42). Cells were stained for: viability (BV510), CD4 (BUV395), and Foxp3 (BV421), all purchased from BD Biosciences, Canada. Cells were blocked with rat α-mouse CD16/32 from Biolegend, USA, surface stained for CD4 and stained intracellularly for Foxp3. Cells were run on an LSRFortessa X-20 flow cytometer and data were analyzed using FlowJo software (v10 & 7.6.5, FlowJo LLC, USA). For analysis, doublets and dead cells were removed from the analysis.

Serum

Blood samples were collected using a terminal cardiac bleed. Blood was left to clot for 30 minutes and then centrifuged twice at 11, 000 g at 4°C for 10 minutes. Serum was collected and used either fresh or stored at -80°C.

Intestinal tissue homogenates

Small intestines were opened longitudinally and washed with PBS to remove luminal content. The mucosal surface was identified under a dissecting microscope. The mucosal surface (with its mucus) was gently scraped using a glass slide. Scrapings were weighed, added to 500 μl lysis buffer (10 μM tris HCl, 0.025% sodium azide, 1% tween 80, 0.02% phenylmethylsulfonyl fluoride) with one complete protease inhibitor tablet (Roche diagnostics GmbH, Germany) and homogenized using a bead beater (40 seconds at speed 6 using the Fast-prep-24 bead beater, MP biomedical). The homogenate was centrifuged at 11, 000 g at 4°C for one hour. Supernatants were collected and used fresh or stored at -80°C.

ELISAs

Cytokines in serum and intestinal tissue homogenates were measured by ELISA according to manufacturer’s guidelines (R & D systems, DY404 kit for IL-4, DY413 kit for IL-13, DY594 kit for IL-21). Total IgE (BD, 555248) and IgA (capture antibody, BD, 556969, detection antibody, BD, 556978) levels were measured by ELISA according to manufacturer’s instructions.

Antigen specific antibody responses were also measured by ELISA. ELISA microplates were coated with 10 μg/ml H. polygyrus antigen in carbonate buffer (0.1mM NaHCO3, pH 9.6), overnight at 4°C. Plates were blocked with 2% BSA in TBS/0.05% tween 20 for 2 hours at 37°C. Sera were diluted in TBS Tween and added to wells overnight at 4°C. Antigen- specific IgG1 was detected with HRP-conjugated corresponding detection antibodies (anti-IgG1 (BD, 553441) with TMB peroxidase substrate (T3405, Sigma). The reaction was stopped using 1M H2SO4 solution and the color change was read at 450nm.

Histology

Consecutive formalin fixed paraffin embedded mouse small intestinal sections were stained with H & E by the University of Calgary’s Veterinary Diagnostic Services Unit. For cell identification (eosinophils and macrophages), one photograph of each granuloma was taken at x400 magnification using an Olympus BX 34 microscope and the Olympus CellSens software. Cells were identified by their morphology and staining patterns.

For immunofluorescence, the sections were stained with either anti-mouse IgG1 (BD 553443), anti-mouse DCLK1 (AB31704), anti-mouse ADAMTS4 (BD, 559354) or their respective isotypes overnight at 4°C. Slides were mounted with FluoroshieldTM with DAPI (Sigma-Aldrich F6057). Images were acquired using the Zeiss Axio ZoomV.16 stereoscopic microscope (Bio Core Facility, Department of Biological Sciences, University of Calgary) or the Thorlabs Tide whole-slide scanning microscope (Live cell imaging facility, Snyder institute of chronic diseases). Brightfield and fluorescence images were taken either under the 2.3x objective with the AxioCam High Resolution color (HRc) and High-Resolution mono (HRm) cameras, or the 20x/0.75 NA objective of the slidescanner respectively. Zeiss Zen (blue edition) software or Thorlabs Tide LS image acquisition software was used. DAPI and FITC fluorophores were used with excitation wavelengths at 359 and 488nm, respectively. Brightness and contrast were adjusted in photoshop.

For Second Harmonic Generation (SHG) Imaging, all samples were imaged using a Thorlabs Bergamo Multiphoton Microscopy Platform (Thorlabs, New Jersey, USA) with a Olympus XLPLan 25X/1.05NA water immersion objective. A Chameleon Ti: Sapphire two-photon laser (Coherent, California, USA) tuned to 900nm was used to collect SHG signal via a PMT with a 447/60 bandpass filter. To ensure consistency and good imaging quality of collagen fibers, laser power and gain were optimized and remained the same throughout all samples imaged. Images from each granuloma were collected in z-stacks of 16 images taken at 1µm intervals. Z-stacks were processed in FIJI (ImageJ version 1.53s) (43) where maximum intensity projections were created analyzed for average pixel intensity.

Nanostring nCounter gene expression assay

Intestinal tissue from naïve mice or dissected pooled granulomas from infected mice were snap frozen in liquid nitrogen and RNA was isolated using phenol-chloroform extraction (TRIZOL, Sigma). RNA was quantified using a nanodrop and 50 ng was used for the Myeloid Innate Immunity V2 panel (NanoString) according to the manufacturer’s guidelines. Gene expression analysis was conducted in R (44). Gene counts obtained via the NanoString hybridization assay were normalized with NanostringNorm (45) using the negative control probes, positive control probes and housekeeping genes Eif2b4, Polr1b, and Edc3. Of the 20 housekeeping genes included in the assay, only Eif2b4, Polr1b, and Edc3 were found to have consistent expression among all samples in preliminary comparisons. Therefore Eif2b4, Polr1b, and Edc3 were the only housekeeping genes used for normalization in subsequent analyses. The normalized counts were then compared using DESeq2 (46) to find differentially expressed genes in pairwise comparisons between treatment groups. A false discovery rate adjusted p-value cut-off of 0.05 and a fold-change cutoff of two were used to identify genes that were differentially expressed in each pairwise comparison. The data discussed in this publication have been deposited in NCBI’s Gene Expression Omnibus (47) and are accessible through GEO (Gene Expression Omnibus) Series accession number GSE164319. Male mice were used for D21 and naive while female mice were used for D7 experiments. Low coverage sequencing of early granulomas indicates no difference in myeloid genes between male and female mice (data not shown).

Statistical analysis (except for nanostring results)

Linearity was assessed using an Anderson Darling test. For nonparametric data, Mann Whitney/Kruskal Wallis tests with Dunn’s multiple comparisons were used to assess differences between either two or more experimental groups using GraphPad Prism. For parametric data, we used T-tests/ANOVA with Sidak’s multiple comparisons.

Results

Increased worm clearance in trickle-infected animals is associated with increased levels of serum IgE, but no other changes in key systemic cytokine or antibody responses

Administering H. polygyrus larvae in low frequent doses to C57Bl/6 mice changed their susceptibility to infection. Two inbred strains of mice, C57Bl/6 (genetically susceptible) and BALB/c (genetically resilient) (13) were infected with H. polygyrus according to the bolus (single 200 larvae dose) or trickle infection regimes (200 larvae dose given over the course of 10 days, Figure 1A). When given a bolus infection of 200 worms, BALB/c mice, being partially resistant to H. polygyrus, eliminated most of their worms by 21 days post-infection (D21, mean of 31, SD +/- 27, Figure 1B). In contrast, C57Bl/6 mice, being susceptible to H. polygyrus, harboured high numbers of adult worms (mean of 88, SD +/- 35, Figure 1B). When infected according to the trickle protocol however, C57Bl/6 mice also eliminated most of the adult worms by 21 days post infection (mean of 44, SD +/- 34, Figure 1B). This difference in worm burden was already apparent at D14 (Supplementary Figure 1B), although many worms in the trickle-infected animals were still developing in the tissue at D14 (Supplementary Figure 1A), skewing the results at this time point.

H. polygyrus clearance has been associated with a strong Th2 response, specifically increases in IL-4 and IL-13 cytokines (32, 48, 49). As the Th2 immune response develops in response to H. polygyrus, MLN (mesenteric lymph nodes) and SPL (spleen) cell numbers increase (50). We found no differences in cell number between the trickle- and bolus-infected groups in either of the organs (Figure 1C). At D14, we observed increased MLN cell numbers in the bolus compared to trickle-infected group (Supplementary Figure 1C). Tregs have been shown to modulate H. polygyrus infection outcome (51), yet we saw no changes in the proportion of Tregs (defined as Foxp3+CD4+ cells) between the trickle and bolus-infected groups at either D14 or D21 (11-16% Foxp3+ cells of CD4+ cells, Supplementary Figure 1D and Figure 1D). We measured the levels of the antigen-specific Th2 cytokine production (IL-4 and IL-13) in both the MLN and SPL as well as in the serum of mice by ELISA (Figures 1E, F). Levels of IL-4 measured in BALB/c mice at D21 post infection were higher than in C57Bl/6 mice (Figure 1E), as has previously been reported (13). No differences were found in cytokine levels between trickle and bolus-infected C57Bl/6 mice at D21 post-infection. Levels of IL-4 and IL-13 were undetectable in the serum, confirming the findings of others (3). In C57Bl/6 animals, we further measured the levels of four cytokines implicated in immune responses against helminths [IFNγ, IL-10, IL-9 and IL-5 (3, 52, 53)] in the MLN and SPL. No difference in these were observed between the trickle and bolus-infected animals (data not shown).

Parasite specific IgG1 and total IgE both increase during primary and secondary H. polygyrus infection (33). IgG1 has been associated with parasite clearance (1) while IgE is thought to reduce parasite fecundity (33). We measured an increase in parasite specific IgG1 by D21 post-infection, as has previously been reported (33). No differences were observed between trickle and bolus-infected animals (Figure 1G). No levels of H. polygyrus antigen-specific IgE, IgG2c or IgA were detectable in the serum of infected mice at any post-infection time point; both larval and adult parasite antigen were tested. However, an increase in total serum IgE was measured in both mouse strains (Figure 1H), with increased levels in the trickle-infected C57Bl/6 animals.

In the small intestine, increased worm clearance in trickle-infected animals is associated with increased tuft cell number

Worm infections result in physiological changes in the small intestine that have been linked to promoting worm expulsion. These include increased tuft cell numbers (54), increased intestinal smooth muscle contractility (55) (and therefore, decreased intestinal transit time) as well as increased mucus production (3). We saw a significant increase in tuft cell numbers three weeks after infection as expected (54) (Figure 2A), from approximately 3 (SD +/- 2.7) cells in naïve animal to more than 22 cells per 5 villi in infected animals. In regions which were not in proximity to granulomas, we also found that trickle-infected animals have a 50% increase in tuft cell number (36, SD +/- 31 vs. 22, SD +/- 17). In areas around the granulomas, this difference was not observed, in accordance with recent data showing that H. polygyrus can inhibit tuft cell expansion (56). Transit time, measured by the time to pass dyed gavaged material, was reduced in D7 bolus-infected (by 18%, SD +/- 7.5) and D21 trickle-infected (by 12%, SD +/- 11) C57Bl/6 animals but not in D21 bolus-infected animals (0%, SD +/- 2.3, Figure 2B). This suggests transit time decreases transiently early in infection. Mucus production, measured indirectly through intestinal tissue weights and goblet cell number, increased with infection but did not differ between the infected groups (Figures 2C, D). The ratio of Muc2/Muc5a gene expression, which increased in trickle-infected Trichuris-infected animals (57), was no different between the H. polygyrus bolus and trickle-infected groups (Figure 2E).

Figure 2
www.frontiersin.org

Figure 2 The reduced worm burden in trickle-infected C57Bl/6 mice is associated with increased tuft cell numbers. 6-8 week old C57Bl/6 mice were infected with 200 H polygyrus larvae according to the bolus and trickle infection regimes. (A) Paraffin embedded swiss rolls were stained with anti-DCLK1. The number of tuft cells were counted in 5 intact villi within the small intestine. Photographs from ten different areas of the small intestine were counted per animal (a minimum of five from areas with no granulomas and a minimum of three from areas with granulomas - Gr). (B) Mice were fasted for 6 hours followed by Evans Blue administration. Time from dye administration to the passing of dyed fecal pellets was measured and normalised to control animals. (C) Small intestines were dissected and scraped using a glass slide leaving only the serosa. The intestinal scrapings were weighed for each mouse and normalised to control animals. (D) Paraffin embedded swiss rolls were stained with Alcian blue. The average number of goblet cells in 5 intact and continuous villi within the small intestine was collected and normalised to the epithelial cell number. (E) RNA was extracted from whole intestinal tissue and Muc2 and Muc5a expression were measured normalised to the β-actin housekeeping gene. (F-J) IL-4, IL-13, RELMα, total IgA and IL-21 were measured in the intestinal scrapings by ELISA. Naive mice (grey circles) and mice infected according to the bolus (black circles) and trickle (white circles) regimes. Graphs represent pooled data from 2 experiments, bars represent the median, with a minimum of 2 mice per group per experiment. A normality test was performed (Anderson-Darling) followed by Kruskal Wallis tests with Dunn’s multiple comparisons test to test for statistical significance between trickle and bolus groups, n.s., not significant, *p<0.05, **p<0.01, ***p<0.001.

We then measured the levels of the Th2 cytokines IL-4 and IL-13 by ELISA in intestinal tissue. They have been associated with stimulating increased mucus production in the small intestine (58). Despite no differences in goblet cell numbers and intestinal weight, IL-4 levels were significantly reduced in trickle compared to bolus-infected animals at D21 (Figure 2F). Levels between D7 bolus-infected and D21 trickle-infected animals were similar, indicating that IL-4 levels are time/dose dependent. IL-13 and RELMα levels, by contrast were increased compared to naïve animals but remained similar between trickle and bolus-infected groups (Figures 2G, H). Levels of IFNγ, IL-5, IL-9 and IL-10 were below the detection limit of the assay for intestinal tissue for all mice tested.

Mucosal IgA levels, regulated by the cytokine IL-21 (59), are also increased in the presence of intestinal dwelling parasites (60). Intestinal IgA levels did not differ between bolus or trickle-infected mice at D21 post-infection (Figure 2I). IL-21 levels were increased at this time point, from 98 pg/ml, SD +/- 84 in naïve animals to approximately 950 pg/ml in infected animals (Figure 2J). However, like IgA levels, they were not different between trickle- and bolus-infected animals.

Increased worm clearance in trickle-infected animals is associated with increased granuloma number, size and IgG levels as well as increased smooth muscle hypertrophy/hyperplasia

Granulomas are a characteristic response to intestinal roundworms and are identifiable by eye (Figure 3A) (13). Bolus-infected BALB/c mice had higher granuloma counts at D21 post-infection (53, SD +/- 17) compared to C57Bl/6 bolus-infected animals (17, SD +/- 12, Figure 3B, left). However, in trickle-infected C57Bl/6 mice, granuloma numbers, like worm burdens, were similar to BALB/c mice (75, SD +/- 18, Figure 3B, left). In C57Bl/6 mice, trickle-infected animals also had larger granulomas (Figure 3B, right) which contained a greater number of eosinophils (Figure 3C, left) but similar numbers of macrophages (Figure 3C, right) compared to bolus-infected animals. To study worm killing mechanisms in the granuloma, we measured levels of IgG by immunofluorescence. At D21 post-infection, no obvious differences in staining were obtained (Figure 3D, left). This is not surprising since at this time point, developing worms have exited the tissue. We also stained for IgG in parasite-containing granulomas from the bolus-infected animals (at D7 when these can be observed) as well as trickle-infected mice (at D14, when these can be observed, Figure 3D). Here, we found a lack of IgG in the granulomas from D7 animals (also undetectable in the serum) compared to strong IgG staining around the developing worms in the granulomas of trickle-infected animals (Figure 3D, right).

Figure 3
www.frontiersin.org

Figure 3 The reduced worm burden in trickle-infected C57Bl/6 mice is associated with increased granuloma size, number and level of IgG as well as intestinal muscle hypertrophy/hyperplasia. 6-8 week old C57Bl/6 and BALB/c mice were infected with 200 H polygyrus larvae according to the bolus and trickle infection regimes. (A) Representative images of granuloma pathology. Representative photographs showing the small intestine of naïve, D21 bolus and D21 trickle-infected C57Bl/6 mice. White arrows point to granulomas. Whole small intestinal swiss rolls were cut into 6 µm paraffin embedded sections and stained with H & E. Representative photographs highlight the granulomas (black arrows). (B) Granulomas were counted on the outside of the small intestine using a dissection microscope for identification (left). Granuloma area was measured on H & E sections (right). (C) Eosinophil (left) and macrophage (right) counts within the center of the granulomas. H & E slides were used to identify eosinophils and macrophages at x400 magnification. Cells were counted for one field of view (FOV) per granuloma. (D) Formalin fixed, paraffin-embedded 6 μm sections were obtained from small intestine swiss rolls. These sections were co-stained with anti-mouse IgG1 and DAPI. Whole sections were studied using the Thorlabs Tide whole-slide scanning microscope (20x objective). Representative granulomas (white dashed line) from D21 (left, no developing worms) and D7 bolus/D14 trickle (right, developing worms present) infected animals with both isotype control (top) and antibody stain (bottom). (E) Left: representative images of smooth muscle cell hypertrophy/hyperplasia on H & E-stained sections. Pink areas around granulomas represent smooth muscle. Black arrows point to areas where collagen density was measured, scale bar = 200um. Right: intestinal smooth muscle area and width were measured on H & E slides for each granuloma (F) Left: representative photographs of collagen within the granulomas using SHG imaging. Right: Collagen density per FOV measured using ImageJ. Data pooled from 2 experiments with a minimum of 2 mice per group per experiment. A normality test was performed (Anderson-Darling) followed by Kruskal Wallis tests with Dunn’s multiple comparisons test and/or an unpaired T-test/Mann-Whitney test to test for statistical significance between trickle and bolus groups, n.s., not significant, **p<0.01, ***p<0.001, ****p<0.0001.

Worm clearance is associated with elevated Th2 responses, which, in a chronic setting, can lead to fibrosis (61). Since intestinal nematodes cause considerable damage to the intestinal mucosa through the creation of granulomas, we wanted to know whether the healing of these structures was affected by the infection type. Using H & E slides (Figure 3E, left), we measured histological abnormalities associated with the granulomas. This included the area and maximum width of smooth muscle (Figure 3E) as well as collagen deposition (Figure 3F) (62). Smooth muscle hypertrophy/hyperplasia was increased in the trickle-infected animals. Although trickle-infected granulomas are also increased in size, we found no correlation between the measured area of the granuloma vs. that of the smooth muscle (R2 = 0.15, data not shown). Fibrosis/Scarring is associated with collagen deposition (14). Using second harmonic generation (SHG) imaging, we assessed the density of collagen in the granulomas (Figure 3F, left). We found no difference between the groups in collagen deposition per field of view (FOV, Figure 3F, right).

Increased worm clearance in trickle-infected animals is associated with a unique gene expression pattern in the granulomas linked to inflammation and tissue scarring

We isolated all granulomas along the small intestine of bolus and trickle-infected C57Bl/6 mice at 21 days post-infection. These are late-stage granulomas, collected 10 days after the last trickle dose, at a point when all worms have developed into adults and had either left the granuloma or been killed within it. To account for the granulomas formed later in the trickle-infected group, we also isolated granulomas from bolus-infected C57Bl/6 mice at 7 days post-infection (early-stage granulomas). To identify the granuloma transcriptional profiles, we extracted the mRNA and quantified transcript levels using the nanostring ‘myeloid innate immunity V2’ panel. For a control, we used naïve mice; as these do not have any granulomas along their small intestine, we harvested intestinal tissue from similar areas to those harvested in infected mice.

Using principal component (PC) analysis, we found that the D21 post-trickle infection group clusters separately from any of the other groups (naïve, D7 and/or D21 post bolus-infection, Figure 4A). Thirty-eight genes were differentially expressed (DE, adjusted p<0.05 and fold change>2) between naïve intestinal tissue (naive group) and granuloma tissue (infected groups trickle and/or bolus) at day 7 and day 21 post-infection. Ten genes were highly expressed (hDE, adjusted p<0.05 and fold change>16) at all infection time points tested (D7B: D7 bolus, D21B: D21 bolus and D21T: D21 trickle) compared to naïve tissue (Table 1, grey rows). All identified hDE genes have already been implicated in the immune response to helminths. Their functions revolve around wound healing (Chil3, Chil4, Mmp12, Serpine1, Retnla, Arg1) and the chemoattraction of eosinophils and alternatively activated macrophages (Rnase2a, Ccl7, Ccl8, Cxcl3). Of the remaining 23 upregulated genes and five downregulated DE genes, only four (Cyp1b1, Plaur, Lamb3 and Hpgd) could not be linked to a previous study in helminths. These genes are involved in the alternatively activated macrophage (AAM) phenotype, extracellular matrix (ECM) remodeling and eicosanoid degradation.

Figure 4
www.frontiersin.org

Figure 4 The reduced worm burden in trickle-infected C57Bl/6 mice is associated with an inflammatory and fibrotic transcriptional profile. 6-8 week old C57Bl/6 mice were infected with 200 H polygyrus larvae according to the bolus and trickle infection regimes. We used the nanostring nCounter mouse myeloid innate immunity V2 panel to measure the transcription profiles of 754 genes within the granulomas. N=2 for naïve mice, n=3 for D7 and D21 bolus-infected animals and n=4 for trickle-infected mice. (A) PCA biplot highlighting gene expression differences between all groups (top) and D21 infected groups (bottom). (B) Heatmap showing the relative expression of differentially expressed genes (FC>2) associated with the regulation of myeloid immune responses in D21 trickle vs. bolus infected animals. In bold, genes that are also differentially expressed from the ones in granulomas from D7 bolus-infected animals. (C) Formalin fixed, paraffin-embedded 6 μm sections were obtained from small intestine swiss rolls. These sections were co-stained with anti-mouse ADAMTS4 or isotype and DAPI. Representative granulomas from bolus- (left) and trickle- (right) infected mice at 21 days post-infection. Antibody stain (bottom), isotype control stain (top). Experiments were performed at least twice (minimum of 2 mice per group) and are representative of a total of 23 (bolus) and 58 (trickle) granulomas. Scale bar = 100μm. (D) String analysis (http://string-db.org) of the differentially expressed genes (FC>2) in bold from the list in (B) was conducted to obtain the network. (E) Gene profiler analysis of molecular function, biological processes and molecular pathways based on gene list from (D).

Table 1
www.frontiersin.org

Table 1 Upregulated genes in all three types of intestinal granulomas (D7 B, D21 B and D21 T, Log2fold>1 in all groups).

At D21 post-infection, 82 upregulated DE genes were identified, of which 26 were highly upregulated hDE genes (grey rows, Table 2) compared to genes expressed at D7 post-infection. In the highly upregulated genes in both bolus and trickle-infected animals: seven were associated with mast cells and basophils (Cpa3, Fcer1a, Ms4a2, Cma1, Tpsb2, Kit, Il3ra), five with transcriptional regulation (Msc, Hdac6, Krba1, Smarcd3, Hoxd4) and two with ECM remodeling (Trem2, Gata2). Of the remaining 56 upregulated genes, 17 were involved in wound healing. Eight genes were downregulated in the D21 granulomas compared to the D7 granulomas (Nox1, Ccl7, Ccl2, Ccl28, Ccl20, Pglyrp1, Ido1, Ptgdr). These are associated with neutrophil and eosinophil chemoattraction and activation.

Table 2
www.frontiersin.org

Table 2 Upregulated genes in intestinal granulomas at 21 days post infection (D21B and D21T, Log2fold>1 in both D21 groups but not in the D7 group).

When looking at differential expression between trickle- and bolus-infected granuloma tissue, we also found striking differences (Figure 4B; Table 3). At D21 post-infection, 17 genes were upregulated in trickle- vs. bolus-infected groups, three of which were hDE. The 2 most upregulated genes were Adamts4 (~x124) and Osm (~x49). They were not upregulated in bolus-infected animals on either D7 or D21 compared to naïve intestinal tissue (fold change<2 and/or p>0.05). ADAMTS proteins, a type of matrix metalloproteinase (MMPs), have been implicated in tissue scarring (63). Interestingly, despite a dramatic increase in Adamts4 expression in the trickle-infected animals (~x124, Table 3), protein levels were increased in bolus but not trickle-infected animals (Figure 4C).

Table 3
www.frontiersin.org

Table 3 Differentially expressed genes between D21B and D21T groups (Log2fold>1 in both groups).

Unsurprisingly, the gene expression profile of infected animals was skewed towards fibrosis and scarring with increased Col1a (seen in all granulomas studied, gene 19 Table 1), increased Tnc (tenacin, increased in D21 vs. D7 granulomas, gene 77 Table 2) and late deposition of Fn1 (fibronectin, increased in D21 vs. D7 granulomas, gene 50 Table 2) (64). A key difference in ECM content in tissue scarring vs. tissue regenerative healing includes low MMP : TIMP ratio (64). This ratio was low in all granulomas but more so in the trickle D21 granulomas: Mmp12 and Mmp13 are increased in both types of D21 granulomas (genes 33 and 70 Table 2), while Timp3 is increased 1.75-fold in trickle vs. bolus D21 granulomas (Supplementary Table 1).

In bolus-infected animals, the granulomas collected at D21 were all of similar ‘age’, generated at the same time in response to one large dose of larvae. In contrast, in the trickle-infected animals, granulomas collected at D21 will have a range of ‘ages’ ranging from 10 to 21 days. To assess whether the differences observed could be attributed to time, we tested whether the differences between the D21 trickle and bolus granulomas were also observed when comparing D7 vs. D21 granulomas. Seven genes were identified (Cxcl5, S100a9, S100a8, H2-q10, Ifit1bl1, Cxcr4 and Kit) where timing likely had an impact. The fold changes for the D21T granulomas fall either between the D7 and D21 bolus granulomas or close to the D7 granuloma values. These genes are involved in events linked to early granuloma functions like cell migration/accumulation and inflammation, or mast cells which are present in late-stage rather than early-stage granulomas. However, 9 genes (Adamts4, Osm, Ndc80, Igf1, Nod2, Adamts12, Smad2, Socs3 and Cx3cl1) are significantly different between D21T vs. D7 and D21T vs. D21B. As such, the difference observed is likely not due to the difference in ‘age’ of the granulomas but can be attributed to the infection regimen (in bold, Figure 4B). String analysis using these 9 genes generated a pathway linking 8 of the genes (Figure 4D). A functional analysis of this pathway using g:Profiler, identified inflammation, tissue morphogenesis and myoblast proliferation as key functions (Figure 4E; Supplementary Table 2).

By contrast, at day 21 post-infection, only seven genes were downregulated in D21T vs. D21B. Of the seven, one was also downregulated between D21T vs. D7B (grey row in Table 3, Kit) and four were downregulated between D21B vs. D7B (in italics in Table 3, Cxcr4, Ptgdr, Lamb3 and Kit). Two genes were not associated with a helminth study (in bold in Table 3, Lamb3 and Insig1).

Discussion

Resistance to H. polygyrus bolus infections is strain dependent with the C57Bl/6 strain being susceptible to H. polygyrus infections and carrying high burden chronic infections. This contrasts with the BALB/c mice which have lower worm burdens and are considered resilient to infection (13). We and others (13, 65) have shown that H. polygyrus-infected BALB/c mice have more granulomas, as well as higher levels of Th2 cytokines and parasite specific IgG1 antibodies compared to susceptible C57Bl/6 mice. These differences contribute to the BALB/c resilient phenotype (13). Despite the BALB/c strain’s resilience to H. polygyrus, we found no differences in BALB/c mice between the two infection models (trickle/bolus), which agrees with findings from Trichuris muris trickle vs. bolus nematode infection studies (57). Therefore timings/dose may have less of an impact in naturally resilient animals compared to more susceptible individuals.

Trickle infections, which mimic the grazing behavior of livestock and continual exposure of humans in endemic areas, result in decreased worm burdens in laboratory animals (40, 41, 62). Using our trickle model, C57Bl/6 mice had fewer worms than those infected with a single bolus infection. Resilience to a bolus H. polygyrus infection has been attributed to a strong Th2 response, requiring tuft cells activation, Th2 cytokine production (IL-4 and 13 in particular), the formation of granulomas, IgG1 production, and the activation of alternatively activated macrophages and mast cells (3, 66). Other changes in intestinal physiology and immune markers have also been observed but not directly linked to resilience (3, 66). In our model, despite the reduced parasite numbers in trickle-infected animals, we were surprised that many of the resilience-associated parameters were unchanged, especially since previous studies using H. polygyrus trickle infection models have linked resilience to increased Il4 expression levels in the MLN and increased serum IgG1 (41, 67).

Our data show that resilience in the trickle-infected C57Bl/6 mice was not associated with increased Th2 cytokine levels (IL-4, 5, 9, 10 and 13) measured in the intestinal content, MLN and/or SPL. We saw no difference in many of the genes associated with typical Th2 anti-H. polygyrus responses. Expression of ECM remodeling genes (Chil4, Chil3, Mmp12, Serpine 1, Retlna, and Arg1), myeloid cell recruitment genes (Ccl7, Ccl8 Cxcl3, Cxcr4, Ccl24, Ccl12 and Ccl2) and genes with direct antiparasitic activity (Retlnb) was similar between all infected groups tested (D7, D21B and D21T).

Goblet cells are responsible for the production and release of mucins that protect the intestinal epithelium against pathogens. During helminth infections, IL-4 and IL-13 stimulate goblet cell hyperplasia, increasing mucin secretion which helps expel intestinal worms (6871). We found an increase in goblet cells and intestinal scrapings weight (composed mainly of mucus) in both bolus- and trickle-infected mice, with no difference in the Muc2/Muc5a gene expression ratio. Our results differ from work using Trichuris trickle-infected mice, where resistance was associated with increased IL-13 and its effector mechanisms including goblet cell hyperplasia and Muc5ac production (57). This difference could be accounted for by the different parasite lifecycles. While they are both enteric, T. muris lives in the caecum and does not fully reside within the intestinal tissue while H. polygyrus can be found in the small intestine either within the tissue or coiled around villi. IL-4 and IL-13 (27), and as such H. polygyrus infection (55), also promote intestinal smooth muscle contraction, thought to promote worm expulsion. Trickle-infected animals had faster intestinal transit times despite lower levels of intestinal IL-4. The decrease in transit time was also observed in bolus-infected animals at D7, where levels of IL-4 were also low, indicating that the effect may be time dependent rather than due to the mode of infection.

Mast cell deficient mice have reduced alarmin (IL-25, IL-33 and TSLP) and Th2 cytokine expression, as well as increased worm burdens (72). Mast cells have also been linked to reduced H. polygyrus fecundity (73). Four of the top five upregulated genes in the D21 (B and T) vs. D7 granuloma areas were genes linked to mast cells (cpa3, Fcer1a, Ms4a2 and Cma1). Since the granulomas used for our studies at D21 did not contain worm larvae, the mast cells present in these late-stage granuloma areas may be participating in the wound healing process. Mast cells have previously been implicated in both cellular and molecular mechanisms of wound healing (reviewed in (74)). Surprisingly, differences in serum IgE levels between bolus and trickle-infected animals were not correlated to differences in Fcer1a gene expression (receptor for the IgE antibody). Interestingly, kit, an essential mast cell developmental and survival factor, is downregulated in the D21T compared to D21B granuloma areas. Since its expression levels are between the levels measured at D7 and D21B, this may be a function of time from granuloma formation rather than an inherent difference between the modes of infection.

Resistant strains of mice develop faster and more intense parasite specific antibody responses following H. polygyrus infections, as compared to susceptible strains, where isotypes IgG1, IgA and IgE have been linked to worm clearance (3, 37, 75). Passive transfer of serum, and specifically IgG1, from infected mice results in decreased adult worm burden and fecundity (67, 7678). H. polygyrus adult numbers are increased in infected mice lacking IgA (79). IgG1 and IgE have also been negatively correlated with worm survival across different strains of mice (8082). We found no differences in IgG1 serum or intestinal IgA levels, and the small increases in serum IgE observed are unlikely to solely account for the worm burden differences between the groups. This again is surprising since others have related increased levels of serum IgG1 to resistance in H. polygyrus trickle infected mice albeit at later time points (41, 67). Eliminating tissue stage parasites is thought to rely on antibody dependent cell mediated cytotoxicity (ADCC) by macrophages and eosinophils (7, 15, 16), the main cellular players within the granuloma. Antibodies are key to this process and we observed the presence of IgG1 in the parasite-containing granulomas of the trickle compared to bolus-infected mice which could account for the improved clearance.

Despite many aspects of the host responses being similar between our two modes of infection, we did observe two significant differences: an increase in tuft cell number and granuloma number in the trickle-infected groups. The tuft cells could be generating a faster response to the incoming larvae, also indicated by the presence of IgG1 around developing worms in the trickle-infected granulomas, leading to more granuloma formation and effective killing of the parasites. Time rather than magnitude of the response may be at play here, which would also explain why all worms in the trickle-infected animals were not cleared. The earlier doses could have avoided the effective anti-tissue dwelling parasite responses that develop over time in the trickle infected animals.

Granulomas eventually disappear once the worms have been killed and/or escaped into the lumen (53). Many studies have focused on understanding the mechanisms involved in damaging/killing worms, but fewer have focused on the wound healing processes involved in creating and resorbing a granuloma. This is key, as there is a delicate balance between parasite clearance and host pathology, which ultimately impacts host fitness. Since trickle-infected mice encounter worms over a period of time compared to the one-time dose in bolus-infected mice, we were particularly interested in the impact of trickle infection on the balance of parasite killing vs. tissue regeneration and scarring in the intestinal tissue. Hyperproliferation/Hyperplasia of intestinal smooth muscle cells leads to a lasting thickening of the intestinal wall. Increases in IGF1, accompanied by altered levels of SOCS3, have been associated with smooth muscle hyperplasia in the context of Crohn’s disease (83). While we saw no differences in the density of collagen deposition or the expression of collagen genes, we did observe an increase in the area of smooth muscle within the granulomas in the trickle-infected animals, associated with increased levels of Igf1, Socs3, Osm, Nod2 and Cx3cl1 in the granuloma areas and a generally more inflammatory profile. In Nippostrongylus brasiliensis infection, IGF1 promotes worm expulsion and acute wound healing. Animals lacking Igf1 had higher worm counts than their wildtype counterparts (84) and IGF1 was associated with acute wound healing and reduced lung damage/hemorrhage during early infection (52). The impact of IGF1 in a more chronic setting or in the context of the small intestine during a helminth infection has yet to be assessed. OSM has been associated with the accumulation of profibrotic macrophages (85). NOD2 KO animals have impaired healing in the skin (86) and in the gut (87). Elevated Nod2 levels in our trickle-infected animals may be due to the ongoing tissue damage that the intestinal mucosa is undergoing with the entry/exit of larval worms spread over time. Increased Cx3cl1 in granulomas has recently been associated with increased levels of Hyaluronic acid (HA) during schistosomiasis (88). HA is a marker of tissue scarring, strengthening the trickle-infected animals’ strong ‘scarring’ phenotype.

To the best of our knowledge, we are the first to implicate the ADAMTS family of proteins (extra cellular matrix degrading enzymes) during helminth infections. Increased levels of the expression of ADAM proteins (Adam8 and Adam19), related to the ADAMTS family, have been measured in Fasciola-infected sheep livers (89). We identified increases in Adamts2, 4, 12 and 17 within D21 granulomas with levels of Adamts4 and 12 being increased in trickle-infected animals. We attempted to correlate scarring to ADAMTS levels, the most highly differentially expressed ADAMTS protein family member in our granuloma areas. However, protein levels did not match the transcriptional profile observed: bolus-infected animals had elevated levels of ADAMTS while trickle-infected animals did not. ADAMTS protein regulation is complex and not yet fully understood (90). We are therefore unable to tell whether the lack of ADAMTS4 in the trickle-infected animals is due to an initial lack of protein or to high protein turn-over. Levels of AGGRECAN protein expression and Versican gene expression, both ADAMTS4 targets, were not detectable in the intestine (data not shown). ADAMTS4 has also been shown to increase tumour growth (91), which correlates with the increased size of the granulomas from trickle-infected animals. More recently, ADAMTS4 was also found to promote chronic airway inflammation by supporting immune cell infiltration at the expense of lung function (92), which correlates with the more inflammatory profile of the granulomas from trickle-infected animals. Further study on the ADAMTS family and its role during granuloma formation/resorption is needed. To fully understand the function of the ADAMTS family, single cell RNA sequencing profiles of early/late-stage granulomas with/without encysted worms and late-stage scar tissue from trickle/bolus-infected mice would provide a more detailed view of the cellular processes underlying the host response to parasitic worms in more natural contexts. This would allow the identification of mechanisms which regulate granuloma formation/resorption and their impact on parasite and host fitness.

Our study highlights the complexities of host-parasite interactions. To study the delicate balance between tissue damage and parasite clearance, we need to use a range of outputs (not just worm burden and systemic Th2 responses) in models which better mimic natural conditions such as trickle infections, re-infections, and co-infections.

Data availability statement

The datasets presented in this study can be found in online repositories. The names of the repository/repositories and accession number(s) can be found in the article/Supplementary Material.

Ethics statement

The animal study was reviewed and approved by University of Calgary’s Life and Environmental Sciences Animal Care Committee (protocol AC17-0083) and the University of California, Riverside’s Institutional Animal Care and Use Committee (https://or.ucr.edu/ori/committees/iacuc.aspx; protocol A-20180023).

Author contributions

Study conception and design: AA, SYK, MGN, and CAMF; data collection: AA, SYK, HL, WNTN, SP, ALC, MCL, JB, ES, and CAMF; analysis and interpretation of results: AA, SYK, HL, SP, SMJP, KDP and CAMF; draft manuscript preparation: AA, SYK, SMJP, JDW, MGN, and CAMF. All authors contributed to the article and approved the submitted version.

Funding

This work was funded through CF’s grants from the Canadian Foundation for Innovation and the Natural Sciences and Engineering Research Council of Canada (NSERC), MGN’s grant from the National Institutes of Health/NIAID (NIH R01AI153195), as well as scholarships for AA (NSERC Create in Host Parasite Interactions, UCalgary FGS International Research Excellence Award, and the Burroughs Wellcome Fund Travel Award), SP (AGES), SMJP (NSERC & Killam Trust), JB (NSERC), MCL (Mitacs Globalinks) and EKS (UCalgary Eyes High).

Acknowledgments

We wish to thank Drs. Vuk Cerovic and Simon Hirota for insightful comments, Sruthi Rajeev and Dr. Derek McKay for their help with DCLK-1 staining, and Drs. Cameron Knight and Carrie Shemanko for the use of their microscopes. We also thank the Live Cell Imaging Laboratory at the Snyder Institute for Chronic Diseases for their technical assistance.

Conflict of interest

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Publisher’s note

All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.

Supplementary material

The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fimmu.2022.1020056/full#supplementary-material

References

1. Hewitson JP, Filbey KJ, Esser-von Bieren J, Camberis M, Schwartz C, Murray J, et al. Concerted activity of IgG1 antibodies and IL-4/IL-25-dependent effector cells trap helminth larvae in the tissues following vaccination with defined secreted antigens, providing sterile immunity to challenge infection. PloS Pathog (2015) 11:1–22. doi: 10.1371/journal.ppat.1004676

CrossRef Full Text | Google Scholar

2. Johnston CJC, Robertson E, Harcus Y, Grainger JR, Coakley G, Smyth DJ, et al. Cultivation of heligmosomoides polygyrus: an immunomodulatory nematode parasite and its secreted products. J Vis Exp (2015) 98:e52412. doi: 10.3791/52412

CrossRef Full Text | Google Scholar

3. Reynolds L, Filbey KJ, Maizels RM. Immunity to the model intestinal helminth parasite heligmosomoides polygyrus. Semin Immunopathol (2012) 34:829–46. doi: 10.1007/s00281-012-0347-3

PubMed Abstract | CrossRef Full Text | Google Scholar

4. Humphreys NE, Xu D, Hepworth MR, Liew FY, Grencis RK. IL-33, a potent inducer of adaptive immunity to intestinal nematodes. J Immunol (2008) 180:2443–9. doi: 10.4049/jimmunol.180.4.2443

PubMed Abstract | CrossRef Full Text | Google Scholar

5. Muzio M, Bosisio D, Polentarutti N, D’amico G, Stoppacciaro A, Mancinelli R, et al. Differential expression and regulation of toll-like receptors (TLR) in human leukocytes: selective expression of TLR3 in dendritic cells. J Immunol (2000) 164:5998–6004. doi: 10.4049/jimmunol.164.11.5998

PubMed Abstract | CrossRef Full Text | Google Scholar

6. Vella AT, Hulsebosch MD, Pearce EJ. Schistosoma mansoni eggs induce antigen-responsive CD44-hi T helper 2 cells and IL-4-secreting CD44-lo cells. potential for T helper 2 subset differentiation is evident at the precursor level. J Immunol (1992) 14:1714–22.

Google Scholar

7. Anthony RM, Urban JF, Alem F, Hamed Rozo H CT, Boucher JL, et al. Memory T(H)2 cells induce alternatively activated macrophages to mediate protection against nematode parasites. Nat Med (2006) 12:955–60. doi: 10.1038/nm1451

PubMed Abstract | CrossRef Full Text | Google Scholar

8. Hewitson J, Murray J, Filbey K, Smith KA, Maizels RM, Hewitson JP, et al. Immune modulation and modulators in heligmosomoides polygyrus infection. Exp Parasitol (2011) 132:76–89. doi: 10.1016/j.exppara.2011.08.011.

PubMed Abstract | CrossRef Full Text | Google Scholar

9. King IL, Mohrs K, Mohrs M. A nonredundant role for IL-21 receptor signaling in plasma cell differentiation and protective type 2 immunity against gastrointestinal helminth infection. J Immunol (2010) 185:6138–45. doi: 10.4049/jimmunol.1001703

PubMed Abstract | CrossRef Full Text | Google Scholar

10. Patnode ML, Bando JK, Krummel MF, Locksley RM, Rosen SD. Leukotriene b 4 amplifies eosinophil accumulation in response to nematodes. J Exp Med (2014) 211:1281–8. doi: 10.1084/jem.20132336

PubMed Abstract | CrossRef Full Text | Google Scholar

11. Pearce EJ, Macdonald AS. The immunobiology of schistosomiasis. Nat Rev Immunol (2002) 2:499–511. doi: 10.1038/nri843

PubMed Abstract | CrossRef Full Text | Google Scholar

12. Morimoto M, Morimoto M, Xiao S, Anthony RM, Star RA, Urban JF, et al. Peripheral CD4 T cells rapidly accumulate at the host:parasite interface during an inflammatory Th2 memory response. J Immunol (2004) 172:2424–30. doi: 10.4049/jimmunol.172.4.2424

PubMed Abstract | CrossRef Full Text | Google Scholar

13. Filbey KJ, Grainger JR, Smith KA, Boon L, van Rooijen N, Harcus Y, et al. Innate and adaptive type 2 immune cell responses in genetically controlled resistance to intestinal helminth infection. Immunol Cell Biol (2014) 92:436–48. doi: 10.1038/icb.2013.109

PubMed Abstract | CrossRef Full Text | Google Scholar

14. Ariyaratne A, Finney C. A. M. C. A. M. Eosinophils and macrophages within the Th2-induced granuloma: Balancing killing and healing in a tight space. Infect Immun (2019) 87:e00127–19. doi: 10.1128/IAI.00127-19

PubMed Abstract | CrossRef Full Text | Google Scholar

15. Wilson MS, Mentink-Kane MM, Pesce JT, Ramalingam TR, Thompson R, Wynn TA. Immunopathology of schistosomiasis. Immunol Cell Biol (2007) 85:148–54. doi: 10.1038/sj.icb.7100014

PubMed Abstract | CrossRef Full Text | Google Scholar

16. Hesse M, Modolell M, la Flamme AC, Schito M, Fuentes JM, Cheever AW, et al. Differential regulation of nitric oxide synthase-2 and arginase-1 by type 1/type 2 cytokines in vivo: granulomatous pathology is shaped by the pattern of l-arginine metabolism. J Immunol (2001) 167:6533–44. doi: 10.4049/jimmunol.167.11.6533

PubMed Abstract | CrossRef Full Text | Google Scholar

17. Song E, Ouyang N, Hörbelt M, Antus B, Wang M, Exton MS. Influence of alternatively and classically activated macrophages on fibrogenic activities of human fibroblasts. Cell Immunol (2000) 204:19–28. doi: 10.1006/cimm.2000.1687

PubMed Abstract | CrossRef Full Text | Google Scholar

18. Barron L, Wynn TA. Macrophage activation governs schistosomiasis-induced inflammation and fibrosis. Eur J Immunol (2011) 41:2509–14. doi: 10.1002/eji.201141869

PubMed Abstract | CrossRef Full Text | Google Scholar

19. Faz-López B, Morales-Montor J, Terrazas LI. Role of macrophages in the repair process during the tissue migrating and resident helminth infections. BioMed Res Int (2016) 2016:8634603. doi: 10.1155/2016/8634603

PubMed Abstract | CrossRef Full Text | Google Scholar

20. Chen G, Wang SH, Jang JC, Odegaard JI, Nair MG. Comparison of RELM-alpha and RELM-beta single- and double-gene- deficient mice reveals that RELM-alpha expression dictates inflammation and worm expulsion in hookworm infection. Infect Immun (2016) 84:1100–11. doi: 10.1128/IAI.01479-15

PubMed Abstract | CrossRef Full Text | Google Scholar

21. Herbert DBR, Hölscher C, Mohrs M, Arendse B, Schwegmann A, Radwanska M, et al. No title. Immunity (2004) 20:623–35. doi: 10.1016/S1074-7613(04)00107-4

PubMed Abstract | CrossRef Full Text | Google Scholar

22. Anthony B, Allen JT, Li YS, McManus DP. Hepatic stellate cells and parasite-induced liver fibrosis. Parasit Vectors (2010) 3:60. doi: 10.1186/1756-3305-3-60

PubMed Abstract | CrossRef Full Text | Google Scholar

23. Friedman SL. Mechanisms of disease: mechanisms of hepatic fibrosis and therapeutic implications. Nat Clin Pract Gastroenterol Hepatol (2004) 1:98. doi: 10.1038/ncpgasthep0055

PubMed Abstract | CrossRef Full Text | Google Scholar

24. Tomasek JJ, Gabbiani G, Hinz B, Chaponnier C, Brown RA. Myofibroblasts and mechano-regulation of connective tissue remodelling. Nat Rev Mol Cell Biol (2002) 3:349. doi: 10.1038/nrm809

PubMed Abstract | CrossRef Full Text | Google Scholar

25. Wynn TA. Common and unique mechanisms regulate fibrosis in various fibroproliferative diseases. J Clin Invest (2007) 117:524–9. doi: 10.1172/JCI31487

PubMed Abstract | CrossRef Full Text | Google Scholar

26. Wynn TA. Cellular and molecular mechanisms of fibrosis. J Pathol (2008) 214:199–210. doi: 10.1002/path.2277

PubMed Abstract | CrossRef Full Text | Google Scholar

27. Zhao A, McDermott J, Urban JF, Gause W, Madden KB, Yeung KA, et al. Dependence of IL-4, IL-13, and nematode-induced alterations in murine small intestinal smooth muscle contractility on Stat6 and enteric nerves. J Immunol (2003) 171:948–54. doi: 10.4049/jimmunol.171.2.948

PubMed Abstract | CrossRef Full Text | Google Scholar

28. Vallance BA, Blennerhassett PA, Collins SM. Increased intestinal muscle contractility and worm expulsion in nematode-infected mice. Am J Physiology-Gastrointest Liver Physiol (1997) 272:G321–7. doi: 10.1152/ajpgi.1997.272.2.G321

CrossRef Full Text | Google Scholar

29. Marillier RG, Brombacher TM, Dewals B, Leeto M, Barkhuizen M, Govender D, et al. IL-4Rα-responsive smooth muscle cells increase intestinal hypercontractility and contribute to resistance during acute schistosomiasis. Am J Physiology-Gastrointest Liver Physiol (2010) 298:G943–51. doi: 10.1152/ajpgi.00321.2009

CrossRef Full Text | Google Scholar

30. Vallance BA, Blennerhassett PA, Deng Y, Matthaei KI, Young IG, Collins SM. IL-5 contributes to worm expulsion and muscle hypercontractility in a primary t. spiralis infection. Am J Physiology-Gastrointest Liver Physiol (1999) 277:G400–8. doi: 10.1152/ajpgi.1999.277.2.G400

CrossRef Full Text | Google Scholar

31. Townsend JM, Fallon GP, Matthews JD, Smith P, Jolin EH, McKenzie NA. IL-9-deficient mice establish fundamental roles for IL-9 in pulmonary mastocytosis and goblet cell hyperplasia but not T cell development. Immunity (2000) 13:573–83. doi: 10.1016/S1074-7613(00)00056-X

PubMed Abstract | CrossRef Full Text | Google Scholar

32. Urban JFJ, Noben-Trauth N, Donaldson DD, Madden KB, Morris SC, Collins M, et al. IL-13, IL-4Ralpha, and Stat6 are required for the expulsion of the gastrointestinal nematode parasite nippostrongylus brasiliensis. Immunity (1998) 8:255–64. doi: 10.1016/S1074-7613(00)80477-X

PubMed Abstract | CrossRef Full Text | Google Scholar

33. McCoy KD, Stoel M, Stettler R, Merky P, Fink K, Senn BM, et al. No title. Cell Host Microbe (2008) 4:362–73. doi: 10.1016/j.chom.2008.08.014

PubMed Abstract | CrossRef Full Text | Google Scholar

34. Esser-von Bieren J, Volpe B, Kulagin M, Sutherland DB, Guiet R, Seitz A, et al. Antibody-mediated trapping of helminth larvae requires CD11b and fcγ receptor I. J Immunol (2015) 194:1154–63. doi: 10.4049/jimmunol.1401645

PubMed Abstract | CrossRef Full Text | Google Scholar

35. Esser-von Bieren J, Mosconi I, Guiet R, Piersgilli A, Volpe B, Chen F, et al. Antibodies trap tissue migrating helminth larvae and prevent tissue damage by driving IL-4R-independent alternative differentiation of macrophages. PloS Pathog (2013) 9:e1003771. doi: 10.1371/journal.ppat.1003771

PubMed Abstract | CrossRef Full Text | Google Scholar

36. Basyoni MMA, Rizk EMA. Nematodes ultrastructure: complex systems and processes. J Parasit Dis (2016) 40:1130–40. doi: 10.1007/s12639-015-0707-8

PubMed Abstract | CrossRef Full Text | Google Scholar

37. Behnke JM, Lowe A, Clifford S, Wakelin D. Cellular and serological responses in resistant and susceptible mice exposed to repeated infection with heligmosomoides polygyrus bakeri. Parasit Immunol (2003) 25:333–40. doi: 10.1046/j.1365-3024.2003.00639.x

CrossRef Full Text | Google Scholar

38. Bancroft AJ, Else KJ, Humphreys NE, Grencis RK. The effect of challenge and trickle trichuris muris infections on the polarisation of the immune response. Int J Parasitol (2001) 31:1627–37. doi: 10.1016/S0020-7519(01)00281-8

PubMed Abstract | CrossRef Full Text | Google Scholar

39. Mihi B, Meulder F, Vancoppernolle S, Rinaldi M, Chiers K, Broeck W. Analysis of the mucosal immune responses induced by single and trickle infections with the bovine abomasal nematode ostertagia ostertagi. Parasite Immunol. (2014) 13:150–6. doi: 10.1111/pim.12094

CrossRef Full Text | Google Scholar

40. Brailsford TJ, Behnke JM. The dynamics of trickle infections with heligmosomoides polygyrus in syngeneic strains of mice. Int J Parasitol (1992) 22:351–9. doi: 10.1016/S0020-7519(05)80013-X

PubMed Abstract | CrossRef Full Text | Google Scholar

41. Colombo S. Polyparasitism of gastrointestinal helminths. University of Manchester (2019).

Google Scholar

42. Cossarizza A, Chang HD, Radbruch A, Acs A, Adam D, Adam-Klages S, et al. Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition). Eur J Immunol (2019) 49:1457–973. doi: 10.1002/eji.201970107

PubMed Abstract | CrossRef Full Text | Google Scholar

43. Schindelin J, Arganda-Carreras I, Frise E, Kaynig V, Longair M, Pietzsch T, et al. Fiji: an open-source platform for biological-image analysis. Nat Methods (2012) 9:676–82. doi: 10.1038/nmeth.2019

PubMed Abstract | CrossRef Full Text | Google Scholar

44. R Core Team. R: A language and environment for statistical computing. r foundation for statistical computing. Vienna, Austria (2020).

Google Scholar

45. Waggott DM. NanoStringNorm: Normalize NanoString miRNA and mRNA data. , R package version 1.2.1.

Google Scholar

46. Love MI, Huber W, Anders S, Love MI, Huber W, Anders S. Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2. Genome Biol (2014) 15:550. doi: 10.1186/s13059-014-0550-8

PubMed Abstract | CrossRef Full Text | Google Scholar

47. Edgar R, Domrachev M, Lash AE. Gene expression omnibus: NCBI gene expression and hybridization array data repository. Nucleic Acids Res (2002) 30:207–10. doi: 10.1093/nar/30.1.207

PubMed Abstract | CrossRef Full Text | Google Scholar

48. Oeser K, Schwartz C, Voehringer D. Conditional IL-4/IL-13-deficient mice reveal a critical role of innate immune cells for protective immunity against gastrointestinal helminths. Mucosal Immunol (2015) 8:672–82. doi: 10.1038/mi.2014.101

PubMed Abstract | CrossRef Full Text | Google Scholar

49. McKenzie GJ, Fallon PG, Emson CL, Grencis RK, McKenzie ANJ. Simultaneous disruption of interleukin (IL)-4 and IL-13 defines individual roles in T helper cell type 2-mediated responses. J Exp Med (1999) 189:1565–72. doi: 10.1084/jem.189.10.1565

PubMed Abstract | CrossRef Full Text | Google Scholar

50. King IL, Mohrs K, Meli AP, Downey J, Lanthier P, Tzelepis F, et al. Intestinal helminth infection impacts the systemic distribution and function of the naïve lymphocyte pool. Mucosal Immunol (2017) 10:1160–8. doi: 10.1038/mi.2016.127

PubMed Abstract | CrossRef Full Text | Google Scholar

51. Smith KA, Filbey KJ, Reynolds LA, Hewitson JP, Harcus Y, Boon L, et al. Low-level regulatory T-cell activity is essential for functional type-2 effector immunity to expel gastrointestinal helminths. Mucosal Immunol (2016) 9:428–43. doi: 10.1038/mi.2015.73

PubMed Abstract | CrossRef Full Text | Google Scholar

52. Chen F, Liu Z, Wu W, Rozo C, Bowdridge S, Millman A, et al. An essential role for the Th2-type response in limiting tissue damage during helminth infection. Nat Med (2012) 18:260. doi: 10.1038/nm.2628

PubMed Abstract | CrossRef Full Text | Google Scholar

53. Progatzky F, Shapiro M, Chng SH, Garcia-Cassani B, Classon CH, Sevgi S, et al. Regulation of intestinal immunity and tissue repair by enteric glia. Nature (2021) 599:125–30. doi: 10.1038/s41586-021-04006-z

PubMed Abstract | CrossRef Full Text | Google Scholar

54. Howitt MR, Lavoie S, Michaud M, Blum AM, Tran SV, Weinstock JV, et al. Tuft cells, taste-chemosensory cells, orchestrate parasite type 2 immunity in the gut. Sci (1979) (2016) 351:1329–33. doi: 10.1126/science.aaf1648

CrossRef Full Text | Google Scholar

55. Shea-donohue T, Notari L, Stiltz J, Sun R, Madden KB, Urban JF Jr, et al. Role of enteric nerves in immune-mediated changes in protease-activated receptor 2 effects on gut function. Neurogastroenterol Motil (2010) 22:1138–e291. doi: 10.1111/j.1365-2982.2010.01557.x

PubMed Abstract | CrossRef Full Text | Google Scholar

56. Drurey C, Lindholm HT, Coakley G, Poveda MC, Löser S, Doolan R, et al. Intestinal epithelial tuft cell induction is negated by a murine helminth and its secreted products. J Exp Med (2022) 219:e20211140. doi: 10.1084/jem.20211140

PubMed Abstract | CrossRef Full Text | Google Scholar

57. Glover M, Colombo SAP, Thornton DJ, Grencis RK. Trickle infection and immunity to trichuris muris. PloS Pathog (2019) 15:e1007926. doi: 10.1371/journal.ppat.1007926

PubMed Abstract | CrossRef Full Text | Google Scholar

58. Fallon PG, Jolin HE, Smith P, Emson CL, Townsend MJ, Fallon R, et al. IL-4 induces characteristic Th2 responses even in the combined absence of IL-5, IL-9, and IL-13. Immunity (2002) 17:7–17. doi: 10.1016/S1074-7613(02)00332-1

PubMed Abstract | CrossRef Full Text | Google Scholar

59. Cao AT, Yao S, Gong B, Nurieva RI, Elson CO, Cong Y. Interleukin (IL)-21 promotes intestinal IgA response to microbiota. Mucosal Immunol (2015) 8:1072–82. doi: 10.1038/mi.2014.134

PubMed Abstract | CrossRef Full Text | Google Scholar

60. Strandmark J, Steinfelder S, Berek C, Kühl AA, Rausch S, Hartmann S. Eosinophils are required to suppress Th2 responses in peyer’s patches during intestinal infection by nematodes. Mucosal Immunol (2016) 2017 10(3):661–72. doi: 10.1038/mi.2016.93

PubMed Abstract | CrossRef Full Text | Google Scholar

61. Gause WC, Wynn TA, Allen JE. Type 2 immunity and wound healing: evolutionary refinement of adaptive immunity by helminths. Nat Rev Immunol (2013) 13:607. doi: 10.1038/nri3476

PubMed Abstract | CrossRef Full Text | Google Scholar

62. Chen W, Lu C, Hirota C, Iacucci M, Ghosh S, Gui X. Smooth muscle Hyperplasia/Hypertrophy is the most prominent histological change in crohn’s fibrostenosing bowel strictures: A semiquantitative analysis by using a novel histological grading scheme. J Crohns Colitis (2017) 11:92–104. doi: 10.1093/ecco-jcc/jjw126

PubMed Abstract | CrossRef Full Text | Google Scholar

63. Kelwick R, Desanlis I, Wheeler GN, Edwards DR. The ADAMTS (A disintegrin and metalloproteinase with thrombospondin motifs) family. Genome Biol (2015) 16(1). doi: 10.1186/s13059-015-0676-3

PubMed Abstract | CrossRef Full Text | Google Scholar

64. Keane TJ, Dziki J, Castelton A, Faulk DM, Messerschmidt V, Londono R, et al. Preparation and characterization of a biologic scaffold and hydrogel derived from colonic mucosa. J BioMed Mater Res B Appl Biomater (2017) 105:291–306. doi: 10.1002/jbm.b.33556

PubMed Abstract | CrossRef Full Text | Google Scholar

65. Finney CAM, Taylor MD, Wilson MS, Maizels RM. Expansion and activation of CD4(+)CD25(+) regulatory T cells in heligmosomoides polygyrus infection. Eur J Immunol (2007) 37:1874–86. doi: 10.1002/eji.200636751

PubMed Abstract | CrossRef Full Text | Google Scholar

66. Vacca F, le Gros G. Tissue-specific immunity in helminth infections. Mucosal Immunol (2022) 2022:1–12. doi: 10.1038/s41385-022-00531-w

CrossRef Full Text | Google Scholar

67. Williams DJ, Behnke JM. Host protective antibodies and serum immunoglobulin isotypes in mice chronically infected or repeatedly immunized with the nematode parasite nematospiroides dubius. Immunology (1983) 48:37–47.

PubMed Abstract | Google Scholar

68. Bogers, Moreels, de Man, Vrolix, Jacobs, Pelckmans, et al. Schistosoma mansoni infection causing diffuse enteric inflammation and damage of the enteric nervous system in the mouse small intestine. Neurogastroenterol Motil (2000) 12:431–40. doi: 10.1046/j.1365-2982.2000.00219.x

PubMed Abstract | CrossRef Full Text | Google Scholar

69. Balemba OB, Semuguruka WD, Hay-Schmidt A, Johansen MV, Dantzer V. Vasoactive intestinal peptide and substance p-like immunoreactivities in the enteric nervous system of the pig correlate with the severity of pathological changes induced by schistosoma japonicum. Int J Parasitol (2001) 31:1503–14. doi: 10.1016/S0020-7519(01)00273-9

PubMed Abstract | CrossRef Full Text | Google Scholar

70. Knight PA, Brown JK, Pemberton AD. Innate immune response mechanisms in the intestinal epithelium: potential roles for mast cells and goblet cells in the expulsion of adult Trichinella spiralis. Parasitology (2008) 135:655–70. doi: 10.1017/S0031182008004319

PubMed Abstract | CrossRef Full Text | Google Scholar

71. Marillier RG, Michels C, Smith EM, Fick LCE, Leeto M, Dewals B, et al. IL-4/IL-13 independent goblet cell hyperplasia in experimental helminth infections. BMC Immunol (2008) 9:11. doi: 10.1186/1471-2172-9-11

PubMed Abstract | CrossRef Full Text | Google Scholar

72. Hepworth MR, Daniłowicz-Luebert E, Rausch S, Metz M, Klotz C, Maurer M, et al. Mast cells orchestrate type 2 immunity to helminths through regulation of tissue-derived cytokines. Proc Natl Acad Sci U.S.A. (2012) 109:6644–9. doi: 10.1073/pnas.1112268109

PubMed Abstract | CrossRef Full Text | Google Scholar

73. Hashimoto K, Uchikawa R, Tegoshi T, Takeda K, Yamada M, Arizono N. Immunity-mediated regulation of fecundity in the nematode heligmosomoides polygyrus – the potential role of mast cells. Parasitology (2010) 137:881–7. doi: 10.1017/S0031182009991673

PubMed Abstract | CrossRef Full Text | Google Scholar

74. Komi DEA, Khomtchouk K, Santa Maria PL. A review of the contribution of mast cells in wound healing: Involved molecular and cellular mechanisms. Clin Rev Allergy Immunol (2020) 58:298–312. doi: 10.1007/s12016-019-08729-w

PubMed Abstract | CrossRef Full Text | Google Scholar

75. Ben-Smith A, Wahid FN, Lammas DA, Behnke JM. The relationship between circulating and intestinal heligmosomoides polygyrus-specific IgG1 and IgA and resistance to primary infection. Parasit Immunol (1999) 21:383–95. doi: 10.1046/j.1365-3024.1999.00236.x

CrossRef Full Text | Google Scholar

76. Dobson C. Passive transfer of immunity with serum in mice infected with nematospiroides dubius: influence of quality and quantity of immune serum. Int J Parasitol (1982) 12:207–13. doi: 10.1016/0020-7519(82)90018-2

PubMed Abstract | CrossRef Full Text | Google Scholar

77. Pritchard DI, Williams DJ, Behnke JM, Lee TD. The role of IgG1 hypergammaglobulinaemia in immunity to the gastrointestinal nematode nematospiroides dubius. the immunochemical purification, antigen-specificity and in vivo anti-parasite effect of IgG1 from immune serum. Immunology (1983) 49:353–65.

PubMed Abstract | Google Scholar

78. Pritchard DI, Behnke JM, Williams DJ. Primary infection sera and IgG1 do not block host-protective immunity to nematospiroides dubius. Immunology (1984) 51:73–81.

PubMed Abstract | Google Scholar

79. McCoy KD, Stoel M, Stettler R, Merky P, Fink K, Senn BM, et al. Polyclonal and specific antibodies mediate protective immunity against enteric helminth infection. Cell Host Microbe (2008) 4:362–73. doi: 10.1016/j.chom.2008.08.014

PubMed Abstract | CrossRef Full Text | Google Scholar

80. Ben-Smith A, Lammas DA, Behnke JM. The relative involvement of Th1 and Th2 associated immune responses in the expulsion of a primary infection of heligmosomoides polygyrus in mice of differing response phenotype. J Helminthol (2003) 77:133–46. doi: 10.1079/JOH2003173

PubMed Abstract | CrossRef Full Text | Google Scholar

81. Wahid FN, Behnke JM. Immunological relationships during primary infection with heligmosomoides polygyrus (Nematospiroides dubius): parasite specific IgG1 antibody responses and primary response phenotype. Parasit Immunol (1993) 15:401–13. doi: 10.1111/j.1365-3024.1993.tb00625.x

CrossRef Full Text | Google Scholar

82. Wahid FN, Behnke JM, Grencis RK, Else KJ, Ben-Smith AW. Immunological relationships during primary infection with heligmosomoides polygyrus: Th2 cytokines and primary response phenotype. Parasitology (1994) 108(Pt 4):461–71. doi: 10.1017/S0031182000076022

PubMed Abstract | CrossRef Full Text | Google Scholar

83. Flynn RS, Murthy KS, Grider JR, Kellum JM, Kuemmerle JF. Endogenous IGF-I and αVβ3 integrin ligands regulate the increased smooth muscle hyperplasia of stricturing crohn’s disease. Gastroenterology (2010) 138:285. doi: 10.1053/j.gastro.2009.09.003

PubMed Abstract | CrossRef Full Text | Google Scholar

84. Spadaro O, Camell CD, Bosurgi L, Nguyen KY, Youm YH, Rothlin CV, et al. IGF1 shapes macrophage activation in response to immunometabolic challenge. Cell Rep (2017) 19:225–34. doi: 10.1016/j.celrep.2017.03.046

PubMed Abstract | CrossRef Full Text | Google Scholar

85. Ayaub EA, Dubey A, Imani J, Botelho F, Kolb MRJ, Richards CD, et al. Overexpression of OSM and IL-6 impacts the polarization of pro-fibrotic macrophages and the development of bleomycin-induced lung fibrosis. Sci Rep (2017) 7:13281. doi: 10.1038/s41598-017-13511-z

PubMed Abstract | CrossRef Full Text | Google Scholar

86. Williams H, Crompton RA, Thomason HA, Campbell L, Singh G, McBain AJ, et al. Cutaneous Nod2 expression regulates the skin microbiome and wound healing in a murine model. J Invest Dermatol (2017) 137:2427–36. doi: 10.1016/j.jid.2017.05.029

PubMed Abstract | CrossRef Full Text | Google Scholar

87. Campbell L, Williams H, Crompton RA, Cruickshank SM, Hardman MJ. Nod2 deficiency impairs inflammatory and epithelial aspects of the cutaneous wound-healing response. J Pathol (2013) 229:121–31. doi: 10.1002/path.4095

PubMed Abstract | CrossRef Full Text | Google Scholar

88. Zhang P, Wang BJ, Wang JZ, Xie XM, Tong QX. Association of CX3CL1 and CX3CR1 expression with liver fibrosis in a mouse model of schistosomiasis. Curr Med Sci (2020) 40:1121–7. doi: 10.1007/s11596-020-2294-x

PubMed Abstract | CrossRef Full Text | Google Scholar

89. Alvarez Rojas CA, Ansell BR, Hall RS, Gasser RB, Young ND, Jex AR, et al. Transcriptional analysis identifies key genes involved in metabolism, fibrosis/tissue repair and the immune response against fasciola hepatica in sheep liver. Parasites Vectors (2015) 8:1–14. doi: 10.1186/s13071-015-0715-7

PubMed Abstract | CrossRef Full Text | Google Scholar

90. Rose KWJ, Taye N, Karoulias SZ, Hubmacher D. Regulation of ADAMTS proteases. Front Mol Biosci (2021) 8:701959. doi: 10.3389/fmolb.2021.701959

PubMed Abstract | CrossRef Full Text | Google Scholar

91. Chen J, Luo Y, Zhou Y, Qin S, Qiu Y, Cui R, et al. Promotion of tumor growth by ADAMTS4 in colorectal cancer: Focused on macrophages. Cell Physiol Biochem (2018) 46:1693–703. doi: 10.1159/000489245

PubMed Abstract | CrossRef Full Text | Google Scholar

92. Ghonim MA, Boyd DF, van de Velde LA, Souquette A, Vogel P, Thomas PG. ADAMTS4 promotes chronic airway inflammation and hyperresponsiveness in HDM-based mouse model of asthma: A potential therapeutic target for asthma traits. J Immunol (2021) 206(1 Supplement):94.20.

Google Scholar

93. Rolot M, Dewals BG. Macrophage activation and functions during helminth infection: Recent advances from the laboratory mouse. J Immunol Res (2018).

PubMed Abstract | Google Scholar

94. Panova V, Gogoi M, Rodriguez-Rodriguez N, Sivasubramaniam M, Jolin HE, Heycock MWD, et al. Group-2 innate lymphoid cell-dependent regulation of tissue neutrophil migration by alternatively activated macrophage-secreted Ear11. Mucosal Immunol (2020) 14(1):26–37.

PubMed Abstract | Google Scholar

95. Jang JC, Chen G, Wang SH, Barnes MA, Chung JI, le GG, et al. Macrophage-derived human resistin is induced in multiple helminth infections and promotes inflammatory monocytes and increased parasite burden. PloS Pathog (2015) 11(1):e1004579.

PubMed Abstract | Google Scholar

96. Dawson HD, Chen C, Li RW, Bell LN, Shea-Donohue T, Kringel H, et al. Molecular and metabolomic changes in the proximal colon of pigs infected with trichuris suis. Sci Rep2020 10(1).

PubMed Abstract | Google Scholar

97. Hu X, Liu X, Li C, Zhang Y, Li C, Li Y, et al. Time-resolved transcriptional profiling of trichinella-infected murine myocytes helps to elucidate host–pathogen interactions in the muscle stage. Parasit Vectors (2021) 14(1):1–17.

PubMed Abstract | Google Scholar

98. Bušelić I, Trumbić Ž, Hrabar J, Vrbatović A, Bočina I, Mladineo I. Molecular and cellular response to experimental anisakis pegreffii (nematoda, anisakidae) third-stage larval infection in rats. Front Immunol (2018) 9:2055.

PubMed Abstract | Google Scholar

99. Nair MG, Du Y, Perrigoue JG, Zaph C, Taylor JJ, Goldschmidt M, et al. Alternatively activated macrophage-derived RELM-alpha is a negative regulator of type 2 inflammation in the lung. J Exp Med (2009) 206(4):937–52.

PubMed Abstract | Google Scholar

100. Pesce JT, Ramalingam TR, Wilson MS, Mentink-Kane MM, Thompson RW, Cheever AW, et al. Retnla (Relmα/Fizz1) suppresses helminth-induced Th2-type immunity. PloS Pathog (2009) 5(4):e1000393.

PubMed Abstract | Google Scholar

101. Wynn TA, Ph D, Barron L. Macrophages: master regulators of inflammation and fibrosis. Semin Liver Dis (2010) 30(3):245–57.

PubMed Abstract | Google Scholar

102. Esser-von Bieren J, Volpe B, Sutherland DB, Bürgi J, Verbeek JS, Marsland BJ, et al. Immune antibodies and helminth products drive CXCR2-dependent macrophage-myofibroblast crosstalk to promote intestinal repair. PloS Pathog (2015) 11(3):e1004778.

PubMed Abstract | Google Scholar

103. Thomas GD, Rückerl D, Maskrey BH, Whitfield PD, Blaxter ML, Allen JE. The biology of nematode- and IL4Rα-dependent murine macrophage polarization in vivo as defined by RNA-seq and targeted lipidomics. Blood 120(25):e93.

PubMed Abstract | Google Scholar

104. Alvarez Rojas CA, Ansell BRE, Hall RS, Gasser RB, Young ND, Jex AR, et al. Transcriptional analysis identifies key genes involved in metabolism, fibrosis/tissue repair and the immune response against fasciola hepatica in sheep liver. Parasit Vectors (2015) 8(1).

PubMed Abstract | Google Scholar

105. Ottow MK, Klaver EJ, van der Pouw Kraan TCTM, Heijnen PD, Laan LC, Kringel H, et al. The helminth trichuris suis suppresses TLR4-induced inflammatory responses in human macrophages. Genes Immun (2014) 15(7):477–86.

PubMed Abstract | Google Scholar

106. Panda SK, Kumar S, Tupperwar NC, Vaidya T, George A, Rath S, et al. Chitohexaose activates macrophages by alternate pathway through TLR4 and blocks endotoxemia. PloS Pathog 8(5):e1002717.

PubMed Abstract | Google Scholar

107. Herbert DR, Yang JQQ, Hogan SP, Groschwitz K, Khodoun M, Munitz A, et al. (2009) 206(13).

Google Scholar

108. Lechner CJ, Komander K, Hegewald J, Huang X, Gantin RG, Soboslay PT, et al. Cytokine and chemokine responses to helminth and protozoan parasites and to fungus and mite allergens in neonates, children, adults, and the elderly. Immun Ageing (2013) 10:1. doi: 10.1186/1742-4933-10-29

PubMed Abstract | CrossRef Full Text | Google Scholar

109. Cortes-Selva D, Elvington AF, Ready A, Rajwa B, Pearce EJ, Randolph GJ, et al. Schistosoma mansoni infection-induced transcriptional changes in hepatic macrophage metabolism correlate with an athero-protective phenotype. Front Immunol (2018), 2580.

PubMed Abstract | Google Scholar

110. Estrada-Reyes ZM, Tsukahara Y, Amadeu RR, Goetsch AL, Gipson TA, Sahlu T, et al. Signatures of selection for resistance to haemonchus contortus in sheep and goats. BMC Genomics (2019) 20(1):1–14. doi: 10.1186/s12864-019-6150-y

PubMed Abstract | CrossRef Full Text | Google Scholar

111. Yan C, Wu J, Xu N, Li J, Zhou QY, Yang HM, et al. TLR4 deficiency exacerbates biliary injuries and peribiliary fibrosis caused by clonorchis sinensis in a resistant mouse strain. Front Cell Infect Microbiol (2021) 0:781.

Google Scholar

112. Chen F, Wu W, Millman A, Craft JF, Chen E, Patel N, et al. Neutrophils prime a long-lived effector macrophage phenotype that mediates accelerated helminth expulsion. Nat Immunol (2014) 15(10):938–46.

PubMed Abstract | Google Scholar

113. Liang YJ, Luo J, Lu Q, Zhou Y, Wu HW, Zheng D, et al. Gene profile of chemokines on hepatic stellate cells of schistosome-infected mice and antifibrotic roles of CXCL9/10 on liver non-parenchymal cells. PloS One 7(8):e42490.

PubMed Abstract | Google Scholar

114. deSchoolmeester ML, Little MC, Rollins BJ, Else KJ. Absence of CC chemokine ligand 2 results in an altered Th1/Th2 cytokine balance and failure to expel trichuris muris infection. J Immunol (2003) 170(9):4693–700.

PubMed Abstract | Google Scholar

115. Periasamy K, Pichler R, Poli M, Cristel S, Cetrá B, Medus D, et al. Candidate gene approach for parasite resistance in sheep – variation in immune pathway genes and association with fecal egg count. PloS One 9(2):88337.

Google Scholar

116. Giacomin PR, Gordon DL, Botto M, Daha MR, Sanderson SD, Taylor SM, et al. The role of complement in innate, adaptive and eosinophil-dependent immunity to the nematode nippostrongylus brasiliensis. Mol Immunol (2008) 45(2):446–55.

PubMed Abstract | Google Scholar

117. Garcia-Campos A, Correia CN, Naranjo-Lucena A, Garza-Cuartero L, Farries G, Browne JA, et al. Fasciola hepatica infection in cattle: Analyzing responses of peripheral blood mononuclear cells (PBMC) using a transcriptomics approach. Front Immunol (2019), 2081.

PubMed Abstract | Google Scholar

118. Siracusa MC, Reece JJ, Joseph F, Urban, Scott AL. Dynamics of lung macrophage activation in response to helminth infection. J Leukoc Biol (2008) 84(6):1422.

PubMed Abstract | Google Scholar

119. Zhou G, Stevenson MM, Geary TG, Xia J. Comprehensive transcriptome meta-analysis to characterize host immune responses in helminth infections. PloS Negl Trop Dis (2016) 10(4).

Google Scholar

120. Geiger S, Jardim-Botelho A, Williams W, Alexander N, Diemert D, Bethony J. Serum CCL11 (eotaxin-1) and CCL17 (TARC) are serological indicators of multiple helminth infections and are driven by schistosoma mansoni infection in humans. Trop Med Int Health (2013) 186.

Google Scholar

121. Rojas-Caraballo J, López-Abán J, Moreno-Pérez DA, Vicente B, Fernández-Soto P, del Olmo E, et al. Transcriptome profiling of gene expression during immunisation trial against fasciola hepatica: identification of genes and pathways involved in conferring immunoprotection in a murine model. BMC Infect Dis 17(1).

PubMed Abstract | Google Scholar

122. Cao S, Gong W, Zhang X, Xu M, Wang Y, Xu Y, et al. Arginase promotes immune evasion of echinococcus granulosus in mice. Parasit Vectors 13(1).

Google Scholar

123. Zhong X, Lundberg M, Råberg L. Comparison of spleen transcriptomes of two wild rodent species reveals differences in the immune response against borrelia afzelii. Ecol Evol 10(13):6421–34. doi: 10.1002/ece3.6377

PubMed Abstract | CrossRef Full Text | Google Scholar

124. Cabantous S, Hou X, Louis L, He H, Mariani O, Sastre X, et al. Evidence for an important role of host microRNAs in regulating hepatic fibrosis in humans infected with schistosoma japonicum. Int J Parasitol (2017) 47(13):823–30.

PubMed Abstract | Google Scholar

125. Xing W, Austen KF, Gurish MF, Jones TG. Protease phenotype of constitutive connective tissue and of induced mucosal mast cells in mice is regulated by the tissue. Proc Natl Acad Sci U.S.A (2011) 108(34):14210–5.

Google Scholar

126. Dessaint JP, Capron A. Fc epsilon receptor II-positive macrophages and platelets: potent effector cells in allergy and defence against helminth parasites. Springer Semin Immunopathol (1990) 12(4):349–63.

Google Scholar

127. Lertanekawattana S, Wichatrong T, Chaisari K, Uchikawa R, Arizono N. Immunological characteristics of patients infected with common intestinal helminths: results of a study based on reverse-transcriptase PCR. (2013) 99(1):71–80. doi: 10.1179/136485905X19892

CrossRef Full Text | Google Scholar

128. Ingham A, Reverter A, Windon R, Hunt P, Menzies M. Gastrointestinal nematode challenge induces some conserved gene expression changes in the gut mucosa of genetically resistant sheep. Int J Parasitol (2008) 38(3–4):431–42.

Google Scholar

129. Li RW, Choudhary RK, Capuco A v, Urban JF. Exploring the host transcriptome for mechanisms underlying protective immunity and resistance to nematode infections in ruminants. Vet Parasitol 190(1–2):1–11. doi: 10.1016/j.vetpar.2012.06.021

CrossRef Full Text | Google Scholar

130. van Meulder F, van Coppernolle S, Borloo J, Rinaldi M, Li RW, Chiers K, et al. Granule exocytosis of granulysin and granzyme b as a potential key mechanism in vaccine-induced immunity in cattle against the nematode ostertagia ostertagi. Infect Immun (2013) 81(5):1798–809.

Google Scholar

131. Kroupova H, Trubiroha A, Wuertz S, Frank SN, Sures B, Kloas W. Nutritional status and gene expression along the somatotropic axis in roach (Rutilus rutilus) infected with the tapeworm ligula intestinalis. Gen Comp Endocrinol (2012) 177(2):270–7.

Google Scholar

132. Sommers CL, Rouquette-Jazdanian AK, Robles AI, Kortum RL, Merrill RK, Li W, et al. miRNA signature of mouse helper T cell hyper-proliferation. PloS One (2013) 8(6).

Google Scholar

133. Donskow-Łysoniewska K, Brodaczewska K, Doligalska M. Heligmosomoides polygyrus antigens inhibit the intrinsic pathway of apoptosis by overexpression of survivin and bcl-2 protein in CD4 T cells. Prion (2013) 7(4):319–27.

Google Scholar

134. Tomita M, Itoh H, Kobayashi T, Onitsuka T, Nawa Y. Expression of mast cell proteases in rat lung during helminth infection: mast cells express both rat mast cell protease II and tryptase in helminth infected lung. Int Arch Allergy Immunol (1999) 120(4):303–9.

Google Scholar

135. Kaifi J, Hall L, Diaz C, Sypek J, Diaconu E, Lass J, et al. Impaired eosinophil recruitment to the cornea in P-Selectin–deficient mice in onchocerca volvulus keratitis (River blindness) | IOVS | ARVO journals. Invest Ophthalmol Vis Sci 41:3856–61.

Google Scholar

136. Shariati F, Pérez-Arellano JL, López-Abán J, Behairy AM, Muro A. Role of angiogenic factors in acute experimental strongyloides venezuelensis infection. Parasite Immunol (2010) 32(6):430–9.

Google Scholar

137. Hise AG, Daehnel K, Gillette-Ferguson I, Cho E, McGarry HF, Taylor MJ, et al. Innate immune responses to endosymbiotic wolbachia bacteria in brugia malayi and onchocerca volvulus are dependent on TLR2, TLR6, MyD88, and mal, but not TLR4, TRIF, or TRAM. J Immunol (2007) 178(2):1068–76.

Google Scholar

138. Inclan-Rico JM, Ponessa JJ, Valero-Pacheco N, Hernandez CM, Sy CB, Lemenze AD, et al. Basophils prime group 2 innate lymphoid cells for neuropeptide-mediated inhibition. Nat Immunol (2020) 21(10):1181–93.

Google Scholar

139. Herbst T, Esser J, Prati M, Kulagin M, Stettler R, Zaiss MM, et al. Antibodies and IL-3 support helminth-induced basophil expansion. Proc Natl Acad Sci U.S.A (2012) 109(37):14954–9.

Google Scholar

140. Ohnmacht C, Voehringer D. Basophils protect against reinfection with hookworms independently of mast cells and memory Th2 cells. J Immunol (2010) 184(1):344–50.

Google Scholar

141. Vaca HR, Celentano AM, Macchiaroli N, Kamenetzky L, Camicia F, Rosenzvit MC. Histone deacetylase enzymes as potential drug targets of neglected tropical diseases caused by cestodes. Int J Parasitol Drugs Drug Resist (2019) 9:120–32.

Google Scholar

142. Turner JD, Pionnier N, Furlong-Silva J, Sjoberg H, Cross S, Halliday A, et al. Interleukin-4 activated macrophages mediate immunity to filarial helminth infection by sustaining CCR3-dependent eosinophilia. PloS Pathog (2018) 14(3).

Google Scholar

143. Mishra BB, Gundra UM, Teale JM. Expression and distribution of toll-like receptors 11–13 in the brain during murine neurocysticercosis. J Neuroinflamm (2008) 5:53.

Google Scholar

144. Batugedara HM, Li J, Chen G, Lu D, Patel JJ, Jang JC, et al. Hematopoietic cell-derived RELMα regulates hookworm immunity through effects on macrophages. J Leukoc Biol (2018) 104(4):855.

Google Scholar

145. Bąska P, Zawistowska-Deniziak A, Norbury LJ, Wiśniewski M, Januszkiewicz K. Fasciola hepatica isolates induce different immune responses in unmaturated bovine macrophages. J Vet Res (2019) 63(1):63.

Google Scholar

146. Andronicos N, Hunt P, Windon R. Expression of genes in gastrointestinal and lymphatic tissues during parasite infection in sheep genetically resistant or susceptible to trichostrongylus colubriformis and haemonchus contortus. Int J Parasitol (2010) 40(4):417–29.

Google Scholar

147. Li Y, Guan X, Liu W, Chen HL, Truscott J, Beyatli S, et al. Helminth-induced production of TGF-β and suppression of graft-versus-Host disease is dependent on IL-4 production by host cells. J Immunol (2018) 201(10):2910–22.

Google Scholar

148. Fumagalli M, Pozzoli U, Cagliani R, Comi GP, Bresolin N, Clerici M, et al. The landscape of human genes involved in the immune response to parasitic worms. BMC Evol Biol (2010) 10(1):264.

Google Scholar

149. McGinty JW, Ting HA, Billipp TE, Nadjsombati MS, Khan DM, Barrett NA, et al. Tuft-Cell-Derived leukotrienes drive rapid anti-helminth immunity in the small intestine but are dispensable for anti-protist immunity. Immunity (2020) 52(3):528–41.

Google Scholar

150. Yang Q, Saenz SA, Zlotoff DA, Artis D, Bhandoola A. Natural helper cells derive from lymphoid progenitors. J Immunol (2011) 187(11):5505.

Google Scholar

151. Lorentsen KJ, Cho JJ, Luo X, Zuniga AN, Urban JF, Zhou L, et al. Bcl11b is essential for licensing Th2 differentiation during helminth infection and allergic asthma. Nat Commun (2018) 9(1):1–14.

Google Scholar

152. Wilkie H, Xu S, Gossner A, Hopkins J. Variable exon usage of differentially-expressed genes associated with resistance of sheep to teladorsagia circumcincta. Vet Parasitol (2015) 212(3–4):206.

Google Scholar

153. Atlija M, Arranz JJ, Martinez-Valladares M, Gutiérrez-Gil B. Detection and replication of QTL underlying resistance to gastrointestinal nematodes in adult sheep using the ovine 50K SNP array. Genet Sel Evol (2016) 48(1):4.

Google Scholar

154. Montero L, Cervantes-Torres J, Sciutto E, Fragoso G. Helminth-derived peptide GK-1 induces Myd88-dependent pro-inflammatory signaling events in bone marrow-derived antigen-presenting cells. Mol Immunol (2020) 128:22–32.

Google Scholar

155. Mohammed AA, Allen JT, Rogan MT. Echinococcus granulosus cyst fluid enhances epithelial-mesenchymal transition. Parasite Immunol (2018) 40(6):e12533.

Google Scholar

156. Lin R, Lü G, Wang J, Zhang C, Xie W, Lu X, et al. Time course of gene expression profiling in the liver of experimental mice infected with echinococcus multilocularis. PloS One (2011) 6(1).

Google Scholar

157. Brown IK, Dyjack N, Miller MM, Krovi H, Rios C, Woolaver R, et al. Single cell analysis of host response to helminth infection reveals the clonal breadth, heterogeneity, and tissue-specific programming of the responding CD4+ T cell repertoire. PloS Pathog (2021) 17(6).

Google Scholar

158. DiNardo AR, Nishiguchi T, Mace EM, Rajapakshe K, Mtetwa G, Kay A, et al. Schistosomiasis induces persistent DNA methylation and tuberculosis-specific immune changes. J Immunol (2018) 201(1):124.

Google Scholar

159. Mukhopadhyay S, Mohanty M, Mangla A, George A, Bal V, Rath S, et al. Macrophage effector functions controlled by bruton’s tyrosine kinase are more crucial than the cytokine balance of T cell responses for microfilarial clearance. J Immunol (2002) 168(6):2914–21.

Google Scholar

160. Prakobwong S, Pinlaor S, Yongvanit P, Sithithaworn P, Pairojkul C, Hiraku Y. Time profiles of the expression of metalloproteinases, tissue inhibitors of metalloproteases, cytokines and collagens in hamsters infected with opisthorchis viverrini with special reference to peribiliary fibrosis and liver injury. Int J Parasitol (2009) 39(7):825–35.

Google Scholar

161. Gurish MF, Tao H, Abonia JP, Arya A, Friend DS, Parker CM, et al. Intestinal mast cell progenitors require CD49dβ7 (α4β7 integrin) for tissue-specific homing. J Exp Med (2001) 194(9):1243.

Google Scholar

162. Erb KJ, Twardzik T, Palmetshofer A, Wohlleben G, Tatsch U, Serfling E. Mice deficient in nuclear factor of activated T-cell transcription factor c2 mount increased Th2 responses after infection with nippostrongylus brasiliensis and decreased Th1 responses after mycobacterial infection. Infect Immun (2003), 71(11)6641.

Google Scholar

163. He X, Wang Y, Fan X, Lei N, Tian Y, Zhang D, et al. A schistosome miRNA promotes host hepatic fibrosis by targeting transforming growth factor beta receptor III. J Hepatol (2020) 72(3):519–27.

Google Scholar

164. Hasby Saad MA, El-Anwar N. Bevacizumab as a potential anti-angiogenic therapy in schistosomiasis: A double-edged, but adjustable weapon. Parasite Immunol (2022) 42(10).

Google Scholar

165. Sekikawa S, Kawai Y, Fujiwara A, Takeda K, Tegoshi T, Uchikawa R, et al. Alterations in hexose, amino acid and peptide transporter expression in intestinal epithelial cells during nippostrongylus brasiliensis infection in the rat. Int J Parasitol (2003) 33(12):1419–26.

Google Scholar

166. Reece JJ, Siracusa MC, Scott AL. Innate immune responses to lung-stage helminth infection induce alternatively activated alveolar macrophages †. Infect Immun (2006) 74(9):4970–81.

Google Scholar

167. Wu Z, Nagano I, Takahashi Y. Candidate genes responsible for common and different pathology of infected muscle tissues between trichinella spiralis and t. pseudospiralis infection. Parasitol Int (2008) 57(3):368–78.

Google Scholar

168. Wang M, Abais JM, Meng N, Zhang Y, Ritter JK, Li PL, et al. Upregulation of cannabinoid receptor-1 and fibrotic activation of mouse hepatic stellate cells during schistosoma j. infection: role of NADPH oxidase. Free Radic Biol Med (2014) 71:109–20.

Google Scholar

169. Gentilini MV, Nuñez GG, Roux ME, Venturiello SM. Trichinella spiralis infection rapidly induces lung inflammatory response: the lung as the site of helminthocytotoxic activity. Immunobiology (2011) 216(9):1054–63.

Google Scholar

170. Myhill LJ, Stolzenbach S, Mejer H, Jakobsen SR, Hansen TVA, Andersen D, et al. Fermentable dietary fiber promotes helminth infection and exacerbates host inflammatory responses. J Immunol (2020) 204(11):3042–55.

Google Scholar

171. Hervé M, Angeli V, Pinzar E, Wintjens R, Faveeuw C, Narumiya S, et al. Pivotal roles of the parasite PGD2 synthase and of the host d prostanoid receptor 1 in schistosome immune evasion. Eur J Immunol (2003) 33(10):2764–72.

Google Scholar

172. Guo Z, Francisco González J, Hernandez JN, Mcneilly TN, Corripio-Miyar Y, Frew D, et al. Possible mechanisms of host resistance to haemonchus contortus infection in sheep breeds native to the canary islands OPEN. Nat Publishing Group (2016).

Google Scholar

173. Asemota OO, Nmorsi OPG, Isaac C, Odoya EM, Akinseye J, Isaac O. Chemokines responses to ascaris lumbricoides sole infection and Co-infection with hookworm among nigerians. N Am J Med Sci (2014) 6(2):84.

Google Scholar

174. Frohberger SJ, Fercoq F, Neumann AL, Surendar J, Stamminger W, Ehrens A, et al. S100A8/S100A9 deficiency increases neutrophil activation and protective immune responses against invading infective L3 larvae of the filarial nematode litomosoides sigmodontis. PloS Negl Trop Dis (2020) 14(2):e0008119. doi: 10.1371/journal.pntd.0008119

CrossRef Full Text | Google Scholar

175. Bowcutt R, Forman R, Glymenaki M, Carding SR, Else KJ, Cruickshank SM. Heterogeneity across the murine small and large intestine. World J Gastroenterol (2014) 20(41):15216–32.

Google Scholar

176. Zhang P, Wang BJ, Wang JZ, Xie XM, Tong QX. Association of CX3CL1 and CX3CR1 expression with liver fibrosis in a mouse model of schistosomiasis. Curr Med Sci (2020) 40(6):1121–7. doi: 10.1007/s11596-020-2294-x

CrossRef Full Text | Google Scholar

177. Herbert DR, Yang JQ, Hogan SP, Groschwitz K, Khodoun M, Munitz A, et al. Intestinal epithelial cell secretion of RELM-β protects against gastrointestinal worm infection. J Exp Med (2009) 206(13):2947–57. doi: 10.1084/jem.20091268

CrossRef Full Text | Google Scholar

178. Grainger JR, Smith KA, Hewitson JP, McSorley HJ, Harcus Y, Filbey KJ, et al. Helminth secretions induce de novo T cell Foxp3 expression and regulatory function through the TGF-β pathway. J Exp Med (2010) 207(11):2331–41.

Google Scholar

179. Webb LM, Lundie RJ, Borger JG, Brown SL, Connor LM, Cartwright AN, et al. Type I interferon is required for T helper (Th) 2 induction by dendritic cells. EMBO J (2017) 36(16):2404.

Google Scholar

180. Shaw EJ, Smith EE, Whittingham-Dowd J, Hodges MD, Else KJ, Rigby RJ. Intestinal epithelial suppressor of cytokine signaling 3 (SOCS3) impacts on mucosal homeostasis in a model of chronic inflammation. Immun Inflammation Dis (2017) 5(3):336.

Google Scholar

181. Hu JS, Freeman CM, Stolberg VR, Chiu BC, Bridger GJ, Fricker SP, et al. AMD3465, a novel CXCR4 receptor antagonist, abrogates schistosomal antigen-elicited (type-2) pulmonary granuloma formation. Am J Pathol (2006) 169(2):424–32.

Google Scholar

182. Layland LE, Mages J, Loddenkemper C, Hoerauf A, Wagner H, Lang R, et al. Pronounced phenotype in activated regulatory T cells during a chronic helminth infection. J Immunol (2010) 184(2):713–24.

Google Scholar

183. Magalhães KG, Luna-Gomes T, Mesquita-Santos F, Corrêa R, Assunção LS, Atella GC, et al. Schistosomal lipids activate human eosinophils via toll-like receptor 2 and PGD2 receptors: 15-LO role in cytokine secretion. Front Immunol (2019) 9.

Google Scholar

184. Shay AE, Diwakar BT, Guan BJ, Narayan V, Urban JF, Prabhu KS. IL-4 up-regulates cyclooxygenase-1 expression in macrophages. J Biol Chem (2017) 292(35):14544–55.

Google Scholar

185. Wu Z, Nagano I, Boonmars T, Takahashi Y. Involvement of the c-ski oncoprotein in cell cycle arrest and transformation during nurse cell formation after trichinella spiralis infection. Int J Parasitol (2006) 36(10–11):1159–66.

Google Scholar

Keywords: helminth, granuloma, trickle infection, ADAMTS, intestinal parasite, tissue scarring

Citation: Ariyaratne A, Kim SY, Pollo SMJ, Perera S, Liu H, Nguyen WNT, Coria AL, Luzzi MC, Bowron J, Szabo EK, Patel KD, Wasmuth JD, Nair MG and Finney CAM (2022) Trickle infection with Heligmosomoides polygyrus results in decreased worm burdens but increased intestinal inflammation and scarring. Front. Immunol. 13:1020056. doi: 10.3389/fimmu.2022.1020056

Received: 15 August 2022; Accepted: 03 November 2022;
Published: 08 December 2022.

Edited by:

Lauren Webb, University of Washington, United States

Reviewed by:

Irah L. King, McGill University, Canada
Juan Inclan Rico, University of Pennsylvania, United States

Copyright © 2022 Ariyaratne, Kim, Pollo, Perera, Liu, Nguyen, Coria, Luzzi, Bowron, Szabo, Patel, Wasmuth, Nair and Finney. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

*Correspondence: Constance A. M. Finney, constance.finney@ucalgary.ca

Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.