AUTHOR=Huang Qian , Wang Chao-Cai , Liu Yun-Guang , Zhao Chang-Ming , Zhang Tian-Ping , Liu Yan , Wang Hua 

TITLE=Clinical relevance of genetic polymorphisms in WNT signaling pathway (SFRP1, WNT3A, CTNNB1, WIF-1, DKK-1, LRP5, LRP6) on pulmonary tuberculosis in a Chinese population

JOURNAL=Frontiers in Immunology

VOLUME=13

YEAR=2022

URL=https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2022.1011700

DOI=10.3389/fimmu.2022.1011700

ISSN=1664-3224

ABSTRACT=<p>The present study was performed to evaluate the association of WNT signaling pathway genes variants with pulmonary tuberculosis (PTB) risk in Chinese Han population. Our study subjects were composed of 452 PTB patients and 465 normal controls, and seventeen SNPs of seven genes in WNT signaling pathway (<italic>SFRP1</italic>, <italic>WNT3A</italic>, <italic>CTNNB1, WIF-1</italic>, <italic>DKK-1</italic>, <italic>LRP5</italic>, <italic>LRP6</italic>) were genotyped by SNPscan technique. We found no significant relationship of <italic>SFRP1</italic> rs10088390, rs4736958, rs3242, <italic>WNT3A</italic> rs752107, rs3121310, <italic>CTNNB1</italic> rs2293303, rs1798802, rs4135385, <italic>WIF-1</italic> rs1026024, rs3782499, <italic>DKK-1</italic> rs2241529, rs1569198, <italic>LRP5</italic> rs3736228, rs556442, <italic>LRP6</italic> rs2302685, rs11054697, rs10743980 polymorphisms with PTB susceptibility. While, <italic>WIF-1</italic> rs3782499 variant was associated with susceptibility to PTB under recessive model, and haplotype analysis showed that <italic>DKK-1</italic> GA haplotype frequency was significantly increased in PTB patients. The <italic>WNT3A</italic> rs3121310, <italic>CTNNB1</italic> rs2293303 polymorphisms were respectively associated with drug-induced liver injury (DILI), sputum smear-positive in PTB patients. The rs3782499 in <italic>WIF-1</italic> gene was related to fever, leukopenia, and the rs1569198 in <italic>DKK-1</italic> was linked to sputum smear-positive in PTB patients. In <italic>LRP5</italic> gene, rs3736228, rs556442 variants respectively affected the occurrence of DILI, fever, and <italic>LRP6</italic> gene rs2302685, rs10743980 variants respectively influenced the development of hypoproteinemia, sputum smear-positive in PTB patients. Our results revealed that WNT signaling pathway genes variation were not associated with the susceptibility to PTB, while <italic>WNT3A</italic>, <italic>CTNNB1</italic>, <italic>WIF-1</italic>, <italic>DKK-1</italic>, <italic>LRP5</italic>, <italic>LRP6</italic> genetic variations might be closely related to the occurrence of several clinical characteristics of PTB patients.</p>