AUTHOR=Jiang Jie , Zhan Xinli , Liang Tuo , Chen Liyi , Huang Shengsheng , Sun Xuhua , Jiang Wenyong , Chen Jiarui , Chen Tianyou , Li Hao , Yao Yuanlin , Wu Shaofeng , Zhu Jichong , Liu Chong 

TITLE=Dysregulation of SAA1, TUBA8 and Monocytes Are Key Factors in Ankylosing Spondylitis With Femoral Head Necrosis

JOURNAL=Frontiers in Immunology

VOLUME=12

YEAR=2022

URL=https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2021.814278

DOI=10.3389/fimmu.2021.814278

ISSN=1664-3224

ABSTRACT=<sec><title>Introduction</title><p>The mechanism of ankylosing spondylitis with femoral head necrosis is unknown, and our study aimed investigate the effects of genetic and immune cell dysregulation on ankylosing spondylitis.</p></sec><sec><title>Materials and Methods</title><p>The protein expression of all ligaments in ankylosing spondylitis with femoral head necrosis was obtained using label-free quantification protein park analysis of six pairs of specimens. The possible pathogenesis was explored using differential protein analysis, weighted gene co-expression network analysis, recording intersections with hypoxia-related genes, immune cell correlation analysis, and drug sensitivity analysis. Finally, routine blood test data from 502 AS and 162 healthy controls were collected to examine immune cell differential analysis.</p></sec><sec><title>Results</title><p>SAA1 and TUBA8 were significantly expressed differentially in these two groups and correlated quite strongly with macrophage M0 and resting mast cells (P &lt; 0.05). Routine blood data showed that monocytes were significantly more expressed in AS than in healthy controls (P &lt; 0.05). SAA1 and TUBA8 were closely related to the sensitivity of various drugs, which might lead to altered drug sensitivity.</p></sec><sec><title>Conclusion</title><p>Dysregulation of SAA1, TUBA8 and monocytes are key factors in ankylosing spondylitis with femoral head necrosis.</p></sec>