AUTHOR=Wang Qilong , Zeng Huikun , Zhu Yan , Wang Minhui , Zhang Yanfang , Yang Xiujia , Tang Haipei , Li Hongliang , Chen Yuan , Ma Cuiyu , Lan Chunhong , Liu Bin , Yang Wei , Yu Xueqing , Zhang Zhenhai TITLE=Dual UMIs and Dual Barcodes With Minimal PCR Amplification Removes Artifacts and Acquires Accurate Antibody Repertoire JOURNAL=Frontiers in Immunology VOLUME=12 YEAR=2021 URL=https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2021.778298 DOI=10.3389/fimmu.2021.778298 ISSN=1664-3224 ABSTRACT=
Antibody repertoire sequencing (Rep-seq) has been widely used to reveal repertoire dynamics and to interrogate antibodies of interest at single nucleotide-level resolution. However, polymerase chain reaction (PCR) amplification introduces extensive artifacts including chimeras and nucleotide errors, leading to false discovery of antibodies and incorrect assessment of somatic hypermutations (SHMs) which subsequently mislead downstream investigations. Here, a novel approach named DUMPArts, which improves the accuracy of antibody repertoires by labeling each sample with dual barcodes and each molecule with dual unique molecular identifiers (UMIs)