AUTHOR=O’Neill Mary B. , Quach Hélène , Pothlichet Julien , Aquino Yann , Bisiaux Aurélie , Zidane Nora , Deschamps Matthieu , Libri Valentina , Hasan Milena , Zhang Shen-Ying , Zhang Qian , Matuozzo Daniela , Cobat Aurélie , Abel Laurent , Casanova Jean-Laurent , Naffakh Nadia , Rotival Maxime , Quintana-Murci Lluis TITLE=Single-Cell and Bulk RNA-Sequencing Reveal Differences in Monocyte Susceptibility to Influenza A Virus Infection Between Africans and Europeans JOURNAL=Frontiers in Immunology VOLUME=12 YEAR=2021 URL=https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2021.768189 DOI=10.3389/fimmu.2021.768189 ISSN=1664-3224 ABSTRACT=
There is considerable inter-individual and inter-population variability in response to viruses. The potential of monocytes to elicit type-I interferon responses has attracted attention to their role in viral infections. Here, we use single-cell RNA-sequencing to characterize the role of cellular heterogeneity in human variation of monocyte responses to influenza A virus (IAV) exposure. We show widespread inter-individual variability in the percentage of IAV-infected monocytes. Notably, individuals with high cellular susceptibility to IAV are characterized by a lower activation at basal state of an IRF/STAT-induced transcriptional network, which includes antiviral genes such as