AUTHOR=Honarmand Tamizkar Kasra , Gorji Pooneh , Gholipour Mahdi , Hussen Bashdar Mahmud , Mazdeh Mehrdokht , Eslami Solat , Taheri Mohammad , Ghafouri-Fard Soudeh 

TITLE=Parkinson’s Disease Is Associated With Dysregulation of Circulatory Levels of lncRNAs

JOURNAL=Frontiers in Immunology

VOLUME=12

YEAR=2021

URL=https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2021.763323

DOI=10.3389/fimmu.2021.763323

ISSN=1664-3224

ABSTRACT=<p>Long non-coding RNAs (lncRNAs) have been recently reported to be involved in the pathoetiology of Parkinson’s disease (PD). Circulatory levels of lncRNAs might be used as markers for PD. In the present work, we measured expression levels of <italic>HULC</italic>, <italic>PVT1</italic>, <italic>MEG3</italic>, <italic>SPRY4-IT1</italic>, <italic>LINC-ROR</italic> and <italic>DSCAM-AS1</italic> lncRNAs in the circulation of patients with PD <italic>versus</italic> healthy controls. Expression of <italic>HULC</italic> was lower in total patients compared with total controls (Expression ratio (ER)=0.19, adjusted P value&lt;0.0001) as well as in female patients compared with female controls (ER=0.071, adjusted P value=0.0004). Expression of <italic>PVT1</italic> was lower in total patients compared with total controls (ER=0.55, adjusted P value=0.0124). Expression of <italic>DSCAM-AS1</italic> was higher in total patients compared with total controls (ER=5.67, P value=0.0029) and in male patients compared with male controls (ER=9.526, adjusted P value=0.0024). Expression of <italic>SPRY4-IT</italic> was higher in total patients compared with total controls (ER=2.64, adjusted P value&lt;0.02) and in male patients compared with male controls (ER=3.43, P value&lt;0.03). Expression of <italic>LINC-ROR</italic> was higher in total patients compared with total controls (ER=10.36, adjusted P value&lt;0.0001) and in both male and female patients compared with sex-matched controls (ER=4.57, adjusted P value=0.03 and ER=23.47, adjusted P value=0.0019, respectively). Finally, expression of <italic>MEG3</italic> was higher in total patients compared with total controls (ER=13.94, adjusted P value&lt;0.0001) and in both male and female patients compared with sex-matched controls (ER=8.60, adjusted P value&lt;0.004 and ER=22.58, adjusted P value&lt;0.0085, respectively). ROC curve analysis revealed that MEG3 and LINC-ROR have diagnostic power of 0.77 and 0.73, respectively. Other lncRNAs had AUC values less than 0.7. Expression of none of lncRNAs was correlated with age of patients, disease duration, disease stage, MMSE or UPDRS. The current study provides further evidence for dysregulation of lncRNAs in the circulation of PD patients.</p>