AUTHOR=Malmberg Henna-Riikka , Hanel Andrea , Taipale Mari , Heikkinen Sami , Carlberg Carsten TITLE=Vitamin D Treatment Sequence Is Critical for Transcriptome Modulation of Immune Challenged Primary Human Cells JOURNAL=Frontiers in Immunology VOLUME=12 YEAR=2021 URL=https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2021.754056 DOI=10.3389/fimmu.2021.754056 ISSN=1664-3224 ABSTRACT=

Microbe-associated molecular patterns, such as lipopolysaccharide (LPS) and β-glucan (BG), are surrogates of immune challenges like bacterial and fungal infections, respectively. The biologically active form of vitamin D, 1α,25-dihydroxyvitamin D₃ (1,25(OH)₂D₃), supports the immune system in its fight against infections. This study investigated significant and prominent changes of the transcriptome of human peripheral blood mononuclear cells that immediately after isolation are exposed to 1,25(OH)₂D₃-modulated immune challenges over a time frame of 24-48 h. In this in vitro study design, most LPS and BG responsive genes are downregulated and their counts are drastically reduced when cells are treated 24 h after, 24 h before or in parallel with 1,25(OH)₂D₃. Interestingly, only a 1,25(OH)₂D₃ pre-treatment of the LPS challenge results in a majority of upregulated genes. Based on transcriptome-wide data both immune challenges display characteristic differences in responsive genes and their associated pathways, to which the actions of 1,25(OH)₂D₃ often oppose. The joined BG/1,25(OH)₂D₃ response is less sensitive to treatment sequence than that of LPS/1,25(OH)₂D₃. In conclusion, the functional consequences of immune challenges are significantly modulated by 1,25(OH)₂D₃ but largely depend on treatment sequence. This may suggest that a sufficient vitamin D status before an infection is more important than vitamin D supplementation afterwards.