AUTHOR=Trailin Andriy , Mrazova Petra , Hruba Petra , Voska Ludek , Sticova Eva , Slavcev Antonij , Novotny Marek , Kocik Matej , Viklicky Ondrej 

TITLE=Chronic Active Antibody-Mediated Rejection Is Associated With the Upregulation of Interstitial But Not Glomerular Transcripts

JOURNAL=Frontiers in Immunology

VOLUME=12

YEAR=2021

URL=https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2021.729558

DOI=10.3389/fimmu.2021.729558

ISSN=1664-3224

ABSTRACT=<p>Molecular assessment of renal allografts has already been suggested in antibody-mediated rejection (ABMR), but little is known about the gene transcript patterns in particular renal compartments. We used laser capture microdissection coupled with quantitative RT-PCR to distinguish the transcript patterns in the glomeruli and tubulointerstitium of kidney allografts in sensitized retransplant recipients at high risk of ABMR. The expressions of 13 genes were quantified in biopsies with acute active ABMR, chronic active ABMR, acute tubular necrosis (ATN), and normal findings. The transcripts were either compartment specific (<italic>TGFB1</italic> in the glomeruli and <italic>HAVCR1</italic> and <italic>IGHG1</italic> in the tubulointerstitium), ABMR specific (<italic>GNLY</italic>), or follow-up specific (<italic>CXCL10</italic> and <italic>CX3CR1</italic>). The transcriptional profiles of early acute ABMR shared similarities with ATN. The transcripts of <italic>CXCL10</italic> and <italic>TGFB1</italic> increased in the glomeruli in both acute ABMR and chronic active ABMR. Chronic active ABMR was associated with the upregulation of most genes (<italic>SH2D1B</italic>, <italic>CX3CR1</italic>, <italic>IGHG1</italic>, <italic>MS4A1</italic>, <italic>C5</italic>, <italic>CD46</italic>, and <italic>TGFB1</italic>) in the tubulointerstitium. In this study, we show distinct gene expression patterns in specific renal compartments reflecting cellular infiltration observed by conventional histology. In comparison with active ABMR, chronic active ABMR is associated with increased transcripts of tubulointerstitial origin.</p>