AUTHOR=An Weiteng , Defaus Sira , Andreu David , Rivera-Gil Pilar 

TITLE=In Vivo Sustained Release of Peptide Vaccine Mediated by Dendritic Mesoporous Silica Nanocarriers

JOURNAL=Frontiers in Immunology

VOLUME=12

YEAR=2021

URL=https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2021.684612

DOI=10.3389/fimmu.2021.684612

ISSN=1664-3224

ABSTRACT=<p>Mesoporous silica nanoparticles have drawn increasing attention as promising candidates in vaccine delivery. Previous studies evaluating silica-based vaccine delivery systems concentrated largely on macromolecular antigens, such as inactivated whole viruses. In this study, we synthesized dendritic mesoporous silica nanoparticles (DMSNs), and we evaluated their effectiveness as delivery platforms for peptide-based subunit vaccines. We encapsulated and tested <italic>in vivo</italic> an earlier reported foot-and-mouth disease virus (FMDV) peptide vaccine (B<sub>2</sub>T). The B<sub>2</sub>T@DMSNs formulation contained the peptide vaccine and the DMSNs without further need of other compounds neither adjuvants nor emulsions. We measured <italic>in vitro</italic> a sustained release up to 930 h. B<sub>2</sub>T@DMSNs-57 and B<sub>2</sub>T@DMSNs-156 released 23.7% (135 µg) and 22.8% (132 µg) of the total B<sub>2</sub>T. The formation of a corona of serum proteins around the DMSNs increased the B<sub>2</sub>T release up to 61% (348 µg/mg) and 80% (464 µg/mg) for B<sub>2</sub>T@DMSNs-57 and B<sub>2</sub>T@DMSNs-156. <italic>In vitro</italic> results point out to a longer sustained release, assisted by the formation of a protein corona around DMSNs, compared to the reference formulation (i.e., B<sub>2</sub>T emulsified in Montanide). We further confirmed <italic>in vivo</italic> immunogenicity of B<sub>2</sub>T@DMSNs in a particle size-dependent manner. Since B<sub>2</sub>T@DMSNs elicited specific immune responses in mice with high IgG production like the reference B<sub>2</sub>T@Montanide™, self-adjuvant properties of the DMSNs could be ascribed. Our results display DMSNs as efficacious nanocarriers for peptide-based vaccine administration.</p>