AUTHOR=Nyanhete Tinashe E. , Edwards Robert J. , LaBranche Celia C. , Mansouri Katayoun , Eaton Amanda , Dennison S. Moses , Saunders Kevin O. , Goodman Derrick , Janowska Katarzyna , Spreng Rachel L. , Zhang Lu , Mudrak Sarah V. , Hope Thomas J. , Hora Bhavna , Bradley Todd , Georgiev Ivelin S. , Montefiori David C. , Acharya Priyamvada , Tomaras Georgia D. TITLE=Polyclonal Broadly Neutralizing Antibody Activity Characterized by CD4 Binding Site and V3-Glycan Antibodies in a Subset of HIV-1 Virus Controllers JOURNAL=Frontiers in Immunology VOLUME=12 YEAR=2021 URL=https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2021.670561 DOI=10.3389/fimmu.2021.670561 ISSN=1664-3224 ABSTRACT=
Broadly neutralizing antibodies (bNAbs), known to mediate immune control of HIV-1 infection, only develop in a small subset of HIV-1 infected individuals. Despite being traditionally associated with patients with high viral loads, bNAbs have also been observed in therapy naïve HIV-1+ patients naturally controlling virus replication [Virus Controllers (VCs)]. Thus, dissecting the bNAb response in VCs will provide key information about what constitutes an effective humoral response to natural HIV-1 infection. In this study, we identified a polyclonal bNAb response to natural HIV-1 infection targeting CD4 binding site (CD4bs), V3-glycan, gp120-gp41 interface and membrane-proximal external region (MPER) epitopes on the HIV-1 envelope (Env). The polyclonal antiviral antibody (Ab) response also included antibody-dependent cellular phagocytosis of clade AE, B and C viruses, consistent with both the Fv and Fc domain contributing to function. Sequence analysis of