AUTHOR=Wen Simin , Wei Hejiang , Liao Qijun , Li Mao , Zhong Shuyi , Cheng Yanhui , Huang Weijuan , Wang Dayan , Shu Yuelong 

TITLE=Identification of Two Novel Candidate Genetic Variants Associated With the Responsiveness to Influenza Vaccination

JOURNAL=Frontiers in Immunology

VOLUME=12

YEAR=2021

URL=https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2021.664024

DOI=10.3389/fimmu.2021.664024

ISSN=1664-3224

ABSTRACT=<sec><title>Background</title><p>Annual vaccination is the most effective prevention of influenza infection. Up to now, a series of studies have demonstrated the role of genetic variants in regulating the antibody response to influenza vaccine. However, among the Chinese population, the relationship between genetic factors and the responsiveness to influenza vaccination has not been clarified through genome-wide association study (GWAS).</p></sec><sec><title>Method</title><p>A total of 1,968 healthy volunteers of Chinese descent were recruited and 1,582 of them were available for the subsequent two-stage analysis. In the discovery stage, according to our inclusion criteria, 123 of 1,582 subjects were selected as group 1 and received whole-genome sequencing to identify potential variants and genes. In the verification stage, 29 candidate variants identified by GWAS were selected for further validation in 481 subjects in group 2. Besides, we also analyzed nine variants from previously published reports in our study.</p></sec><sec><title>Results</title><p>Multivariate logistic regression analysis showed that compared with the TT genotype of <italic>ZBTB46</italic> rs2281929, the TC + CC genotype was associated with a lower risk of low responsiveness to influenza vaccination adjusted for gender and age (Group 2: <italic>P</italic> = 7.75E-05, OR = 0.466, 95%CI = 0.319–0.680; Combined group: <italic>P</italic> = 1.18E-06, OR = 0.423, 95%CI = 0.299–0.599). In the combined group, <italic>IQGAP2</italic> rs2455230 GC + CC genotype was correlated with a lower risk of low responsiveness to influenza vaccination compared with the GG genotype (<italic>P</italic> = 8.90E-04, OR = 0.535, 95%CI = 0.370–0.774), but the difference was not statistically significant in group 2 (<italic>P</italic> = 0.008). The antibody fold rises of subjects with <italic>ZBTB46</italic> rs2281929 TT genotype against H1N1, H3N2,and B were all significantly lower than that of subjects with TC + CC genotype (<italic>P</italic> &lt; 0.001). Compared with <italic>IQGAP2</italic> rs2455230 GC + CC carriers, GG carriers had lower antibody fold rises to H1N1 (<italic>P</italic> = 0.001) and B (<italic>P</italic> = 0.032). The GG genotype of rs2455230 tended to be correlated with lower antibody fold rises (<italic>P</italic> = 0.096) against H3N2, but the difference was not statistically significant. No correlation was found between nine SNPs from previously published reports and the serological response to influenza vaccine in our study.</p></sec><sec><title>Conclusion</title><p>Our study identified two novel candidate missense variants, <italic>ZBTB46</italic> rs2281929 and <italic>IQGAP2</italic> rs2455230, were associated with the immune response to influenza vaccination among the Chinese population. Identifying these variants will provide more evidence for future research and improve the individualized influenza vaccination program.</p></sec>