AUTHOR=Van Zeebroeck Lauren , Arroyo Hornero Rebeca , Côrte-Real Beatriz F. , Hamad Ibrahim , Meissner Torsten B. , Kleinewietfeld Markus TITLE=Fast and Efficient Genome Editing of Human FOXP3+ Regulatory T Cells JOURNAL=Frontiers in Immunology VOLUME=12 YEAR=2021 URL=https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2021.655122 DOI=10.3389/fimmu.2021.655122 ISSN=1664-3224 ABSTRACT=
FOXP3+ regulatory T cells (Tregs) are central for maintaining peripheral tolerance and immune homeostasis. Because of their immunosuppressive characteristics, Tregs are a potential therapeutic target in various diseases such as autoimmunity, transplantation and infectious diseases like COVID-19. Numerous studies are currently exploring the potential of adoptive Treg therapy in different disease settings and novel genome editing techniques like CRISPR/Cas will likely widen possibilities to strengthen its efficacy. However, robust and expeditious protocols for genome editing of human Tregs are limited. Here, we describe a rapid and effective protocol for reaching high genome editing efficiencies in human Tregs without compromising cell integrity, suitable for potential therapeutic applications. By deletion of