AUTHOR=Pievani Alice , Savoldelli Roberto , Poelchen Juliane , Mattioli Elisa , Anselmi Giorgio , Girardot Alice , Utikal Jochen , Bourdely Pierre , Serafini Marta , Guermonprez Pierre 

TITLE=Harnessing Mesenchymal Stromal Cells for the Engineering of Human Hematopoietic Niches

JOURNAL=Frontiers in Immunology

VOLUME=12

YEAR=2021

URL=https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2021.631279

DOI=10.3389/fimmu.2021.631279

ISSN=1664-3224

ABSTRACT=<p>Tissue engineering opens multiple opportunities in regenerative medicine, drug testing, and modeling of the hematopoiesis in health and disease. Recapitulating the organization of physiological microenvironments supporting leukocyte development is essential to model faithfully the development of immune cells. Hematopoietic organs are shaped by spatially organized niches defined by multiple cellular contributions. A shared feature of immune niches is the presence of mesenchymal stromal cells endowed with unique roles in organizing niche development, maintenance, and function. Here, we review challenges and opportunities in harnessing stromal cells for the engineering of artificial immune niches and hematopoietic organoids recapitulating leukocyte ontogeny both <italic>in vitro</italic> and <italic>in vivo</italic>.</p>