AUTHOR=Dernstedt Andy , Leidig Jana , Holm Anna , Kerkman Priscilla F. , Mjösberg Jenny , Ahlm Clas , Henriksson Johan , Hultdin Magnus , Forsell Mattias N. E. 

TITLE=Regulation of Decay Accelerating Factor Primes Human Germinal Center B Cells for Phagocytosis

JOURNAL=Frontiers in Immunology

VOLUME=11

YEAR=2021

URL=https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2020.599647

DOI=10.3389/fimmu.2020.599647

ISSN=1664-3224

ABSTRACT=<p>Germinal centers (GC) are sites for extensive B cell proliferation and homeostasis is maintained by programmed cell death. The complement regulatory protein Decay Accelerating Factor (DAF) blocks complement deposition on host cells and therefore also phagocytosis of cells. Here, we show that B cells downregulate DAF upon BCR engagement and that T cell-dependent stimuli preferentially led to activation of DAF<sup>lo</sup> B cells. Consistent with this, a majority of light and dark zone GC B cells were DAF<sup>lo</sup> and susceptible to complement-dependent phagocytosis, as compared with DAF<sup>hi</sup> GC B cells. We could also show that the DAF<sup>hi</sup> GC B cell subset had increased expression of the plasma cell marker Blimp-1. DAF expression was also modulated during B cell hematopoiesis in the human bone marrow. Collectively, our results reveal a novel role of DAF to pre-prime activated human B cells for phagocytosis prior to apoptosis.</p>