AUTHOR=Lu Wei-Jia , Zhou Li , Gao Fan-Xiang , Zhou Yu-Lin , Li Zhi , Zhang Xiao-Juan , Wang Yang , Gui Jian-Fang 

TITLE=Dynamic and Differential Expression of Duplicated Cxcr4/Cxcl12 Genes Facilitates Antiviral Response in Hexaploid Gibel Carp

JOURNAL=Frontiers in Immunology

VOLUME=11

YEAR=2020

URL=https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2020.02176

DOI=10.3389/fimmu.2020.02176

ISSN=1664-3224

ABSTRACT=<p>Chemokine receptor <italic>cxcr4</italic> and its ligand <italic>cxcl12</italic> have evolved two paralogs in the teleost lineage. In this study, we have identified four duplicated <italic>cxcr4</italic> and <italic>cxcl12</italic> genes from hexaploid gibel carp, <italic>Carassius gibelio</italic>, respectively. <italic>Cgcxcr4b</italic>s and <italic>Cgcxcl12a</italic>s were dynamically and differentially expressed in immune-related tissues, and significantly up-regulated in head kidney and spleen after crucian carp herpesvirus (<italic>Ca</italic>HV) infection. Blocking Cxcr4/Cxcl12 axis by injecting AMD3100 brought more severe bleeding symptom and lower survival rate in <italic>Ca</italic>HV-infected fish. AMD3100 treatment also suppressed the up-regulation of key antiviral genes in head kidney and spleen, and resulted in more acute replication of <italic>Ca</italic>HV in <italic>vivo</italic>. Consistently, the similar suppression of up-regulated expression of key antiviral genes were also observed in CAB cells treated by AMD3100 after poly(I:C) stimulation. Finally, MAPK3 and JAK/STAT were identified as the possible pathways that <italic>Cg</italic>Cxcr4s and <italic>Cg</italic>Cxcl12s participate in to promote the antiviral response in <italic>vitro</italic>.</p>