AUTHOR=Cha Hefei , Xie Hongyan , Jin Chenxi , Feng Yuanfa , Xie Shihao , Xie Anqi , Yang Quan , Qi Yanwei , Qiu Huaina , Wu Qiongli , Yin Zhinan , Mu Jianbing , Huang Jun 

TITLE=Adjustments of γδ T Cells in the Lung of Schistosoma japonicum-Infected C56BL/6 Mice

JOURNAL=Frontiers in Immunology

VOLUME=11

YEAR=2020

URL=https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2020.01045

DOI=10.3389/fimmu.2020.01045

ISSN=1664-3224

ABSTRACT=<p>Many kinds of lymphocytes are involved in <italic>Schistosoma japonicum</italic> (<italic>S. japonicum</italic>) infection-induced disease. γδ T cells comprise a small number of innate lymphocytes that quickly respond to foreign materials. In this study, the role of γδ T cells in the lung of <italic>S. japonicum</italic>-infected C56BL/6 mice was investigated. The results demonstrated that <italic>S. japonicum</italic> infection induces γδ T cell accumulation in the lung, expressing higher levels of CD25, MHCII, CD80, and PDL1, and lower levels of CD127 and CD62L (<italic>P</italic> &lt; 0.05). The intracellular cytokines staining results illustrated higher percentages of IL-4-, IL-10-, IL-21-, and IL-6-producing γδ T cells and lower percentages of IFN-γ-expressing γδ T cells in the lung of infected mice (<italic>P</italic> &lt; 0.05). Moreover, the granuloma size in lung tissue was significantly increased in Vδ<sup>−/−</sup> mice (<italic>P</italic> &lt; 0.05). In the lung of <italic>S. japonicum</italic>-infected Vδ<sup>−/−</sup> mice, both type 1 and type 2 immune responses were decreased significantly (<italic>P</italic> &lt; 0.05). In addition, the expression of CD80 and CD69 on B cells was decreased significantly (<italic>P</italic> &lt; 0.05), and the SEA-specific antibody was markedly decreased (<italic>P</italic> &lt; 0.05) in the blood of infected Vδ<sup>−/−</sup> mice. In conclusion, this study indicates that γδ T cells could adjust the Th2 dominant immune response in the lung of <italic>S. japonicum</italic>-infected mice.</p>