AUTHOR=Harly Christelle , Kenney Devin , Wang Yueqiang , Ding Yi , Zhao Yongge , Awasthi Parirokh , Bhandoola Avinash 

TITLE=A Shared Regulatory Element Controls the Initiation of Tcf7 Expression During Early T Cell and Innate Lymphoid Cell Developments

JOURNAL=Frontiers in Immunology

VOLUME=11

YEAR=2020

URL=https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2020.00470

DOI=10.3389/fimmu.2020.00470

ISSN=1664-3224

ABSTRACT=<p>The transcription factor TCF-1 (encoded by <italic>Tcf7</italic>) plays critical roles in several lineages of hematopoietic cells. In this study, we examined the molecular basis for <italic>Tcf7</italic> regulation in T cells, innate lymphoid cells, and migratory conventional dendritic cells that we find express <italic>Tcf7</italic>. We identified a 1 kb regulatory element crucial for the initiation of <italic>Tcf7</italic> expression in T cells and innate lymphoid cells, but dispensable for <italic>Tcf7</italic> expression in <italic>Tcf7</italic>-expressing dendritic cells. Within this region, we identified a Notch binding site important for the initiation of <italic>Tcf7</italic> expression in T cells but not in innate lymphoid cells. Our work establishes that the same regulatory element is used by distinct transcriptional controllers to initiate <italic>Tcf7</italic> expression in T cells and ILCs.</p>