AUTHOR=Cohen Lianne B. , Lindsay Scott A. , Xu Yangyang , Lin Samuel J. H. , Wasserman Steven A. 

TITLE=The Daisho Peptides Mediate Drosophila Defense Against a Subset of Filamentous Fungi

JOURNAL=Frontiers in Immunology

VOLUME=11

YEAR=2020

URL=https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2020.00009

DOI=10.3389/fimmu.2020.00009

ISSN=1664-3224

ABSTRACT=<p>Fungal infections, widespread throughout the world, affect a broad range of life forms, including agriculturally relevant plants, humans, and insects. In defending against fungal infections, the fruit fly <italic>Drosophila melanogaster</italic> employs the Toll pathway to induce a large number of immune peptides. Some have been investigated, such as the antimicrobial peptides (AMPs) and Bomanins (Boms); many, however, remain uncharacterized. Here, we examine the role in innate immunity of two related peptides, Daisho1 and Daisho2 (formerly IM4 and IM14, respectively), found in hemolymph following Toll pathway activation. By generating a CRISPR/Cas9 knockout of both genes, Δ<italic>daisho</italic>, we find that the Daisho peptides are required for defense against a subset of filamentous fungi, including <italic>Fusarium oxysporum</italic>, but not other Toll-inducible pathogens, such as <italic>Enterococcus faecalis</italic> and <italic>Candida glabrata</italic>. Analysis of null alleles and transgenes revealed that the two <italic>daisho</italic> genes are each required for defense, although their functions partially overlap. Generating and assaying a genomic epitope-tagged Daisho2 construct, we detected interaction <italic>in vitro</italic> of Daisho2 peptide in hemolymph with the hyphae of <italic>F. oxysporum</italic>. Together, these results identify the Daisho peptides as a new class of innate immune effectors with humoral activity against a select set of filamentous fungi.</p>