AUTHOR=Schaffert Anja , Hanić Maja , Novokmet Mislav , Zaytseva Olga , Krištić Jasminka , Lux Anja , Nitschke Lars , Peipp Matthias , Pezer Marija , Hennig René , Rapp Erdmann , Lauc Gordan , Nimmerjahn Falk TITLE=Minimal B Cell Extrinsic IgG Glycan Modifications of Pro- and Anti-Inflammatory IgG Preparations in vivo JOURNAL=Frontiers in Immunology VOLUME=10 YEAR=2020 URL=https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2019.03024 DOI=10.3389/fimmu.2019.03024 ISSN=1664-3224 ABSTRACT=
Select residues in the biantennary sugar moiety attached to the fragment crystallizable of immunoglobulin G (IgG) antibodies can modulate IgG effector functions. Thus, afucosylated IgG glycovariants have enhanced cytotoxic activity, whereas IgG glycovariants rich in terminal sialic acid residues can trigger anti-inflammatory effects. More recent evidence suggests that terminal α2,6 linked sialic acids can be attached to antibodies post IgG secretion. These findings raise concerns for the use of therapeutic antibodies as they may change their glycosylation status in the patient and hence affect their activity. To investigate to what extent B cell extrinsic sialylation processes modify therapeutic IgG preparations