AUTHOR=Pilon Caroline , Stehlé Thomas , Beldi-Ferchiou Asma , Matignon Marie , Thiolat Allan , Burlion Aude , Grondin Cynthia , Birebent Brigitte , Pirenne France , Rouard Hélène , Lang Philippe , Marodon Gilles , Grimbert Philippe , Cohen José L. 

TITLE=Human Apoptotic Cells, Generated by Extracorporeal Photopheresis, Modulate Allogeneic Immune Response

JOURNAL=Frontiers in Immunology

VOLUME=10

YEAR=2019

URL=https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2019.02908

DOI=10.3389/fimmu.2019.02908

ISSN=1664-3224

ABSTRACT=<p>The induction of specific and sustainable tolerance is a challenging issue in organ transplantation. The discovery of the immunosuppressive properties of apoptotic cells in animal models has paved the way for their use in human transplantation. In this work, we aimed to define a stable, reproducible, and clinically compatible production procedure of human apoptotic cells (Apo-cells). Using a clinically approved extracorporeal photopheresis technique, we have produced and characterized phenotypically and functionally human apoptotic cells. These Apo-cells have immunosuppressive properties proved <italic>in vitro</italic> and <italic>in vivo</italic> in NOD/SCID/γC mice by their capacity to modulate an allogeneic response following both a direct and an indirect antigen presentation. These results brought the rationale for the use of Apo-cells in tolerance induction protocol for organ transplantation.</p>