AUTHOR=Stachyra Anna , Zawistowska-Deniziak Anna , Basałaj Katarzyna , Grzelak Sylwia , Gondek Michał , Bień-Kalinowska Justyna 

TITLE=The Immunological Properties of Recombinant Multi-Cystatin-Like Domain Protein From Trichinella Britovi Produced in Yeast

JOURNAL=Frontiers in Immunology

VOLUME=10

YEAR=2019

URL=https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2019.02420

DOI=10.3389/fimmu.2019.02420

ISSN=1664-3224

ABSTRACT=<p>Trichinellosis is a globally-distributed zoonotic parasitic disease caused by nematode worms of the genus <italic>Trichinella</italic>. One of the most common species of <italic>Trichinella</italic> known to affect human health is <italic>T. britovi</italic>; however, it is relatively poorly investigated. A thorough knowledge of the proteins expressed by <italic>Trichinella</italic> is important when developing immunological detection methods and vaccines and studying its interactions with the host. The present study uses the <italic>Pichia pastoris</italic> expression system to produce a soluble TbCLP antigen which induces strong antibody responses in the host during natural infection. Our results demonstrate the feasibility of TbCLP antigen production in yeasts, which are able to carry out post-translational modifications such as glycosylation and disulfide bond formation; they also indicate that the glycosylated TbCLP antigen had immunogenic effects in the tested mice and induced a mixed Th1/Th2 response, and was associated with a reduced larval burden after challenge with <italic>T. britovi</italic>. Subsequent <italic>in vitro</italic> stimulation of mice splenocytes revealed that TbCLP most likely possesses immunomodulatory properties and may play a significant role in the early phase of infection, affecting host immunological responses.</p>