AUTHOR=Wang Shenghua , Geng Na , Zhou Dong , Qu Yi , Shi Mengke , Xu Yuliang , Liu Kangping , Liu Yongxia , Liu Jianzhu 

TITLE=Oral Immunization of Chickens With Recombinant Lactobacillus plantarum Vaccine Against Early ALV-J Infection

JOURNAL=Frontiers in Immunology

VOLUME=10

YEAR=2019

URL=https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2019.02299

DOI=10.3389/fimmu.2019.02299

ISSN=1664-3224

ABSTRACT=<p>In this study, a novel oral vaccine of recombinant <italic>Lactobacillus plantarum</italic> (<italic>L. plantarum</italic>) containing the gp85 protein was explored, and the effects of this vaccine on the prevention of subgroup J Avian Leukosis Virus (ALV-J) infection were assessed. In the current study, the gp85 protein of ALV-J was expressed on the surface of <italic>L. plantarum</italic> with the surface-display motif, <italic>pgsA</italic>, by constructing a shuttle vector <italic>pMG36e:pgsA:gp85</italic>. Surface localization of the fusion protein was verified by western blotting and flow cytometry. Subsequently, Specific Pathogen Free Hy-Line Brown layer chickens were orally vaccinated with the recombinant <italic>L. plantarum</italic> and presented with high levels of serum immunoglobulin G (IgG) and secretory immunoglobulin A (sIgA) titers in bile and duodenal-mucosal fluid. After challenged with ALV-J of a 3 × 10<sup>3</sup> 50% tissue culture infective dose (TCID50), serum samples of the chickens were collected and viremia was analyzed. Results showed that, compared to the <italic>L. plantarum</italic> and PBS control group, the recombinant <italic>L. plantarum</italic> group showed a significant rise in antibody levels after inoculation, and provide improved protection against ALV-J according to viremia detection. These results indicate that oral immunization with the recombinant <italic>L. plantarum</italic> provided an effective means for eliciting protective immune response against early ALV-J infection.</p>