AUTHOR=Wosen Jonathan E. , Ilstad-Minnihan Alexandra , Co Julia Y. , Jiang Wei , Mukhopadhyay Dhriti , Fernandez-Becker Nielsen Q. , Kuo Calvin J. , Amieva Manuel R. , Mellins Elizabeth D. 

TITLE=Human Intestinal Enteroids Model MHC-II in the Gut Epithelium

JOURNAL=Frontiers in Immunology

VOLUME=10

YEAR=2019

URL=https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2019.01970

DOI=10.3389/fimmu.2019.01970

ISSN=1664-3224

ABSTRACT=<p>The role of intestinal epithelial cells (IECs) in mucosal tolerance and immunity remains poorly understood. We present a method for inducing MHC class II (MHC-II) in human enteroids, “mini-guts” derived from small intestinal crypt stem cells, and show that the intracellular MHC-II peptide-pathway is intact and functional in IECs. Our approach enables human enteroids to be used for novel <italic>in vitro</italic> studies into IEC MHC-II regulation and function during health and disease.</p>