AUTHOR=Lanz Tobias V. , Pröbstel Anne-Katrin , Mildenberger Iris , Platten Michael , Schirmer Lucas 

TITLE=Single-Cell High-Throughput Technologies in Cerebrospinal Fluid Research and Diagnostics

JOURNAL=Frontiers in Immunology

VOLUME=10

YEAR=2019

URL=https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2019.01302

DOI=10.3389/fimmu.2019.01302

ISSN=1664-3224

ABSTRACT=<p>High-throughput single-cell technologies have recently emerged as essential tools in biomedical research with great potential for clinical pathology when studying liquid and solid biopsies. We provide an update on current single-cell methods in cerebrospinal fluid research and diagnostics, focusing on high-throughput cell-type specific proteomic and genomic technologies. Proteomic methods comprising flow cytometry and mass cytometry as well as genomic approaches including immune cell repertoire and single-cell transcriptomic studies are critically reviewed and future directions discussed.</p>